Adrian G. R.

@ndchiappini @GracePanetti Phen + menthol is my go-to for most organolithium/grignard tbh, but what do you mean by "it only does total base"? As in, e.g. hydroxide impurities will interfere, or am I misunderstanding?

@madras_bertha @zenbrainest There are washing steps prior to ligand addition, so one can argue that the 5-HT will mostly be gone. I think the main worry would be receptor desensitization/internalization induced by high [5-HT].

@zenbrainest Yes, biogenic amines in serum is old story. uM 5-HT is huge. Ki of 5-HT for 5-HT2A ~ 20 nM (depending on radioligand) implying that receptor could be fully occupied by serum 5-HT.

Interesting although you should have used serum free media as even 2% serum has uM 5ht.

@zenbrainest Yeah, that's a weird "control" experiment. Should have compared it to dialyzed serum.

@Colemanomonas @ATinyGreenCell Ofc depends on desired info, but Isothermal Titration Calorimetry (ITC) is nice and label-free, tho data analysis can sometimes be less trivial. If you just want to know how much binds, then dialysis with MW cut-off between mRNA and protein might work similar to your proposal.

Can anyone suggest an easy way to measure mRNA binding to a protein? Would like to avoid radioactive labels and complicated protocols. thinking of just binding the (His-tag) proteins to nickel column, running RNA thru it and measuring A260 of eluate vs control. Too simplistic?

@ndchiappini Thanks! Maybe I'll buy some if EU shipping isn't super expensive.

@ndchiappini Are those aluminum granules I see? Where did you get them? I really wanted to buy some Lab Armor alu beads for heat baths, but they're ridiculously priced, and the bulk aluminum granules I bought were too fine and rough to be useful.

@KRHornberger Best solution I found for us who use Firefox (works on a lot of publisher sites): twitter.com/softnucleophil…

What the hell is this popping up in journals everywhere now, and why do you hate it?

@Robert_Palgrave There's a decent comment on the chemistry at PubPeer too (#5" target="_blank" rel="nofollow noopener">pubpeer.com/publications/8…). I'm no solid state chemist, but their experimentals and characterization seem pretty dodgy tbh. Obv just a preprint, but I'm very doubtful. Extraordinary claims, extraordinary evidence, etc.

There is a lot of excitement about a possible new room temperature superconductor. A lot of physicists have weighed in on the superconducting results but I haven't seen much comment on the material itself and how they made it. So here's my take 🧵 twitter.com/alexkaplan0/st…

@MoleculesGoHard @AmerChemSociety Tip: Use extension "Redirector" (@einaregilsson) in Firefox/Chrome, and add rule(s) to make it redirect epdf to pdf. Rule below/in pic might only work for certain sites, but works for ACS journals atm. RegEx pattern: ^(.*)/doi/epdf/(.*) Redir to: $1/doi/pdf/$2

Hey @AmerChemSociety I'm clicking pdf but I'm getting epdf. Please make it stop

@JImmatSci So the same team has observed phosphine again? Is that it? I remember people questioning quite a bit whether any kind of life is really even necessary for phosphine gas production. Anyone, please enlighten me.

Looks like it’s time to wheel out this classic paper again…

@BagPhos @AmerChemSociety I am appalled.

Some people are going to have thoughts on how the @AmerChemSociety draws benzene #BondsVsCircle

@chem_alexrob @Chemjobber I'm a bit surprised at people's response, I have to admit. I don't think I've ever cleaned an NMR tube for reuse.

So far the biggest shock I’ve experience post grad school working in industry is that NMR tubes and vials are thrown out after a single use. You’re telling me I don’t ever have to scrub vials again?!!

@zenbrainest @sequencemyneuro That's something the research community should aim to change, though. I know it's difficult to get funding for such, but if one has a little bit of extra resources, just pre-print publish it. Don't really have to prove a negative, just that it wasn't able to be replicated.

@sequencemyneuro Most people, including myself, do not expend effort publishing 'non replications' as it is very difficult (if not impossible) to prove a negative. Typically if results are insufficiently robust to be widely replicated they fade from view...eventually.

Well-put by one of the authors of the recent psychedelic TrkB (BDNF receptor) paper! They did not claim that psychedelics are BDNF agonists. They are allostatic modulators that increase the activity of TrkB when it binds to BDNF

@SamesLab @scisignal Cool paper, but doesn't really refute much? While I'm not entirely convinced by the Castrén article, it only claims positive allosteric modulation, not "direct activation". Am I missing something?

This paper claims psychedelics induce neuroplasticity via direct activation of the BDNF receptor TrkB nature.com/articles/s4159… Before you reach a conclusion, see our paper @scisignal; 40K+ compounds were tested, none activates TrkB! science.org/doi/10.1126/sc… #psychedelics

@Maxime_R_Vitale @atkim3 Would bet 15-40 μm or thereabouts – at least that's the typical particle size ime.

@atkim3 Normal silica or 30-40 angstroms ?

DCVC is an amazing technique - this is 4g of crude material and I got almost TLC-level separation in just 8 fractions

@radscientist_ Fair enough, all our hotplates look like hot garbage anyway lol

@softnucleophile just keeps the gross chemicals from spilling right onto the heat source!

black and yellow black and yellow black and yellow black and yellow #realtimechem

@Explosions_Fire Like, adding my NMR spectra as SVG made it more than triple in file size and now I have to wait for like 5 min each time it autosaves

@Explosions_Fire Currently writing my MSc thesis in Word, and god damn it makes me wish I had just started out with LaTeX smh

I actually love MicroSoft Word. I love right clicking and selecting Update Field on every figure caption. I get joy from Assigning Headings in Define New Multilevel List

@ndchiappini I bet it's because OsO4 is sometimes used to stain tissue for TEM (iirc), and bio ppl sometimes have powdered milk for stuff like making blocking solutions for ELISA or something