Tao Dong

Should Infectious Disease Clinicians Be Alarmed?--Functional activation reveals a repressor-gated fourth type VI secretion system in clinical Pseudomonas aeruginosa biorxiv.org/content/10.648…

@earthcurated @CrispeThe

Post a picture YOU took. Just a pic. No description.

A phospholipase effector of the type VI secretion system modulates plant reproduction | mBio journals.asm.org/doi/10.1128/mb… Excited to share how T6SS impacts plant reproduction. Please check out "why we did it" in the Importance section :)

@erhminlai Thank you!

@taogdong Congratulations! Great to see this exciting finding published in Cell Reports!

T6SS assembly starts from a droplet! cell.com/cell-reports/f… ；I am excited to share this story. It was first submitted in Oct 2023 and has been reviewed by multiple reviewers/editors/journals. It's a struggling but valuable learning experience. Many thanks to everyone involved.

Some campus view of SUSTech and a city view taken with my phone.

Great opportunity for a fully funded exchange PhD at the SUSTech for up to two years. Eligibility: Full-time PhD students from the top 150 universities who have completed all required courses at their home institutions. Visit fellow.sustech.edu.cn/#/fellowship/e…

I am very excited to share this work done by my PhD student Tongtong. With an eye for detail, she uncovered fascinating behaviors within a colony grown on a standard LB plate. "Filamentous prophage Pf4 promotes genetic exchange in Pseudomonas aeruginosa" academic.oup.com/ismej/article/…

Please apply or RT for these two PhD positions in our Institute !

@_JimHorne Jim, too many ideas but just don't know where to begin 😅. I hope we will have a sequel about this and just have to convince a student to pick up some trails that are left by a former postdoc who has made some interesting findings.

@taogdong So LB can be even more problematic than Nikaido says (schaechter.asmblog.org/schaechter/200…)! Any idea what could cause the large batch to batch variation?

What LB plates you use is a matter of life or death to bacteria. For details, see our newest ISME J paper: Abiotic factors modulate interspecies competition mediated by the type VI secretion system effectors in Vibrio cholerae doi.org/10.1038/s41396…

@surt_lab While doing this, we did try different suppliers for tryptone, yeast extract, and agar and mixed them in different combos when making plates. Indeed, the sources do matter. We followed on the obvious one but there are other things in the plates that still remain puzzling.

@DrShawnL Related, Shawn. We ran LB on a DNA gel and found some bands back in 2014 :)

@taogdong So is this consistent with the eDNA/chelator induction of T6 in Pseudomonas, where low cations = on, high cations = off?

@hongbing_yu Thanks, Hongbing.

@taogdong Congrats! I love the story so much.

@SineSvenningsen @taodong Thank you Sine. I will forward your nice words to my student Mingxuan. She will be happy hearing this :)

@ZhaoQingLuo Welcome to Twitter 😃

Legionella pneumophila temporally regulates the activity of ADP/ATP translocases by reversible ADP‐ribosylation onlinelibrary.wiley.com/doi/full/10.10… A new biochemical trick by Legionella on a new platform: mLife. My first ever tweet😀!

This is the behind-the-paper blog. "From liking watermelon to liking a watermelon pathogen " microbiologycommunity.nature.com/posts/from-lik… #behindthepaper

Very excited to share that we published our new story "T6SS penetration into Gram+ cells" in the first issue of a new microbiology journal mLife. doi.org/10.1002/mlf2.1…

This is an expanded version of the original discovery that we shared in bioRxiv in March 2021 (doi.org/10.1101/2021.0…)