Dakota Becker-Greene

@denujak @AikawaElena It is definitely possible, as there have been a few large scale studies that have already taken this approach, measuring either S100A8/9 or S100A9 in human serum samples. The heterodimer has been implicated in autoimmune thyroid disorders and arthritis, just to name a few.

@becker_greene @AikawaElena Nice! How feasible is it to measure homo-/hetro-dimer in serum in large scale in humans?

It gives me great pleasure to host today's #AskTheATVBFirstAuthors with @quant_scientist, @koga_ichiro and @becker_greene& @AikawaElena. @atvbahajournals @hanrui_zhang @YogenKanthi @clintomics @KhendiWhite @GomezLabPitt1 @JoePlatelet @TessaJBarrett @KateyRayner @theclotthickens

@denujak @AikawaElena Taken together, these results suggested that the homodimer (S100A9) has a greater binding affinity and a more significant role in the RAGE pathway than the heterodimer (S100A8/9), which was also supported by previous literature, DOI: 10.1371/journal.pbio.1000097.

@becker_greene @AikawaElena Is it possible that Mac EVs-containing s100A8/9 are taken up by neighbouring Macs to propagate inflammation/osteogenic activity? #S100A9_RageAxis #CalcificEVsinDiabetesMellitus #VascularCalcification #AskTheATVBFirstAuthors ahajournals.org/doi/10.1161/AT…

@denujak @AikawaElena Additionally, there were limited osteogenic effects, as only Runx2 was increased at the mRNA level and no other osteogenic markers showed significant effects (Supplemental Fig. IV).

Last paper for #AskTheATVBFirstAuthors today is a cool study by @becker_greene & @AikawaElena, showing macrophage-S100A9-RAGE promotes extracellular vesicle microcalcification in diabetes @atvbahajournals @dkoprivamd @MarkCBlaser @CICSnews @sapassoslivia @toleafgirl

@denujak @AikawaElena Thank you for the question @denujak, as you bring up an important point. When we explored the possible effects on inflammation and osteogenic activity, we found that treatment with S100A8/9 protein in vitro only increased inflammatory markers (TNFa and IL1-B).

Very excited to be apart of this discussion and give more insight into our recent paper on the role of the S100A9-RAGE axis in EV-mediated microcalcification development

Check out our new publication. Looking forward to leading a Q&A session with #ATVB discussing our recent publication on July 2nd, from 9 AM - 9 PM.