Nader

1/7 First of all, big shoutout to co-authors on modeling (@MKarimzade, @neal_ravindra, @RexMa9, @HAOTIANCUI1, @LeeTaliq), huge appreciation to data generation (Lexi, @alerasool, Adam) and bioinformatics team (@_annhuang), and leadership for vision and direction (@BoWang87, @inCiChu)! Preprint is now live on bioRxiv: biorxiv.org/content/10.648… All models start from high-quality data.

Our latest paper is out! In collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/ cell.com/cell/fulltext/…

Our latest work on developing a potent and specific tool for activating or repressing endogenous splicing events is out! authors.elsevier.com/c/1jJci3vVUPRm…

Graduate student Jack Li's work out in Molecular Cell now: By conducting a screen of 341 splicing factors, Jack identified RBM25 coupled to dCasRx as a superior tool to boost inclusion of endogenous exons /1

Check out our CRISPR genome-scale exon perturbation study @MolecularCell. We identified exons influencing cell fitness and uncovered a novel mechanism by which #splicing affects #transcription. @Michael_Aregger @dlarun88 @meisheng_xiao @NCIResearchCtr @NIH doi.org/10.1016/j.molc…

Our paper on functional discovery of protein degradation and stabilization effectors with proteome-scale induced proximity screens is out in its final version! 1/ nature.com/articles/s4158… rdcu.be/dBUju

early winter @newlimit: - >10X more reprogramming factor sets tested than ever before - >2M cells profiled, 10TB+ of raw seq data - +3 team members - 2X speed up in reprogramming library build times - +1 cell types on our Engine - 1st rejuvenating hits

📜 Happy to share our recent preprint featuring ORFtag - a versatile, easy and cost-effective method for probing protein function at a proteome scale. This work is the result of a collaborative effort by the @stark_lab, @AmeresSL, @EllingUlrich and @juliusbrennecke labs. 1/2

2023 @newlimit: - 50X more reprogramming factor sets tested than the field before us - 20X factor demultiplexing performance (reading), 7X factor delivery (writing) - 2X team size - +3 functional assays for immunology program - +2 in silico model metrics > SOTA blog ⬇️

Induxion is looking for a Research Associate/Technician to support its platform activities - Located in Toronto. jobrxiv.org/job/induxion-t…

early autumn @newlimit: - 6X'd the # of reprogramming factors we've tested - >4X'd signal-to-noise for our core pooled screening assay - shortened feedback cycles by 40% - improved in silico reprog models >30% all covered in our latest blog

Help me find my stolen bike! bikeindex.org/bikes/1692267

Our latest preprint is out! In a fun collaboration with @DrAnneCarpenter, we conducted a systematic high-content screen to understand the role of protein mislocalization in diverse human disorders. 1/8 biorxiv.org/content/10.110…

@DonnellyCentre @UofT @mike_tilapia @MoGen_Grad Thank you so much @DonnellyCentre! & bug thanks to the glorious leader @mike_tilapia, who doesn’t just fight like a dairy farmer but is full of innovative ideas and is an amazing supervisor. Miss you all ❤️

Congratulations to Nader Alerasool, a #PhD graduate from the Taipale lab at @UofT! 🎉 Nader received the Donnelly Centre Research #Thesis Prize for his groundbreaking research on transcriptional activators and targeted gene repression. Learn more 👉 bit.ly/2023ThesisPrize

Ever wondered if you could read 100s of metabolites and drugs in parallel using a @illumina or a @10X platform? But metabolites aren’t nucleic acids — how can that work? Check out our new preprint and read on for a thread: biorxiv.org/content/10.110….

We're excited to announce that we've raised our Series A from @_DimensionCap, @foundersfund, & @kleinerperkins blog.newlimit.com/p/newlimit-ser…

New preprint! 🗞️ We used pooled protein screens to develop compact human transcriptional effectors that work across diverse biological contexts to activate or silence gene expression biorxiv.org/content/10.110…

last month @newlimit: - 1st reprogramming screens in primary cells - Immunology team 3X'd - 1st model-driven hypotheses this month: - improving screen efficiency: more exps/{$, time} - multi-modal in silico reprogramming models all this & more in our April update ⬇️

Our paper on 14-3-3 proteins and their role in protein aggregation and phase separation is out! This paper represents the main PhD thesis work by my student Dmitri Segal. 1/ cell.com/molecular-cell…

last month @newlimit: - largest partial reprogramming screen yet - 50% more TFs to test - first in silico reprogramming models this month: - screens in old donor cells - library rebuild -- 10X manufacturing efficiency - automating screen analysis to shorten DBTL loops