The Hiebert Lab

Our latest work is out now in @CellGenomics. @HillaryLayden and team use the dTAG system and deep genomic analysis to determine how wild-type and mutant FOXO1 control transcription in DLBCL. cell.com/cell-genomics/…

Congrats to Anna and Scott for bringing home the Frank Chytil Travel Award and the Richard Armstrong Mentoring Award from yesterday's @VandyBiochem Departmental Award reception!

Mutant FOXO1 controls an oncogenic network via enhancer accessibility dlvr.it/T5QPKD

@FengXiao1988 @CellGenomics @HillaryLayden We haven’t tested dTAG-13 in culture so we can’t comment on that. For us dTAG-v1 (targets VHL) is more effective than dTAG-47 (targets CRBN) at degrading most of the proteins we have tagged. So we would recommend v1 out of the two but results will vary based on target.

@HiebertLab @CellGenomics @HillaryLayden I am wondering if dTAG-13 works better than dTAG-47. Which one is better in your hands?

Our latest work is out now in @CellGenomics. @HillaryLayden and team use the dTAG system and deep genomic analysis to determine how wild-type and mutant FOXO1 control transcription in DLBCL. cell.com/cell-genomics/…

I'm excited to announce that I'm defending on February 14th at 1 pm. See what I've uncovered about how mutations in FOXO1 contribute to B-cell lymphomas! Zoom link available; message me.

We spent more time than we'd like to admit staring at adapter sequences, but we've put together a protocol for dual-indexed PRO-seq library prep. Based off of the TRUseq Small RNA kit, the protocol can be easily adapted for other small RNA applications. researchsquare.com/article/rs-311…

@Susia_ss It helps our core keep costs down and improve turnaround time I believe.

Has anyone updated their PRO-seq/GRO-seq adapters/sequencing primers to include dual indices for better results on the NovaSeq? Our current protocol is based on the TRUseq Small RNA kit but our core would like us to include a second index for better multiplexing. #Genomics

We're hiring! If you're interested in dissecting the mechanism of action of transcriptional regulators in cancer apply here: jobs.sciencecareers.org/job/637660/pos…

Check out our latest story in @MolecularCell. Our team pairs the dTAG system with deep genomic analysis to determine the spatiotemporal role of SMARCA5 in the maintenance of chromatin structure and function. bit.ly/3GNCalx

Mark your calendar for our next dissertation defense! Monica Bomber @monica_bomber from the @HiebertLab will be defending her thesis on Thursday, January 19th at 1 pm in LH 208!

Very happy to welcome Susu Zhang @SusuZhang95 to our lab. She completed her PhD studies with Scott Hiebert @hiebertlab at Vanderbilt. Welcome to the lab, Susu!

Excited to share our newest story! Our team details how the fusion transcription factor PAX3-FOXO1 regulates RNAPII pause release at a small number of genes via the recruitment of co-regulatory complexes to specific enhancer elements in aRMS cells. shorturl.at/ceFHL

Very excited to share our work on PAX3-FOXO1 transcription regulation in aRMS now out in @MolecularCell. A huge thank you to everyone who supported and contributed to this project!!! @HiebertLab

@TaylorJ_MD Low 70s and sunny for the most part! Might rain on Wednesday though.

How’s the weather in #Nashville? Looking forward to joining colleagues tomorrow through Friday for #EvansMDS22 #MDSsm #leusm

The Department of Biochemistry @VandyBiochem in Vanderbilt Basic Sciences @VUBasicSciences is recruiting new faculty! Apply here: apply.interfolio.com/112443

Another dissertation defense to announce! Susu Zhang (@SusuZhang95) from the @HiebertLab will be defending tomorrow, June 1, at 10:30 am in LH 512. She will be presenting about the transcriptional control of oncogenic transcription factor fusions in tumor development.

@benoitbruneau @generegulation This one? Out of the Tansey lab. WDR5 and HDM2 perturbation in rhabo lines. ncbi.nlm.nih.gov/pmc/articles/P…

Hivemind: I'm looking for a very recent paper where a chromatin remodeler or TF was disrupted and there was a broad reduction in ribosomal protein production (at least 64 down) and a resulting effect on protein production. I remember the figures but can't locate the paper. Thx

Thinking about a postdoc @VandyBiochem? Check out our great researchers and the Destination Biochemistry postdoc scholar award. Full stipend + $10K bonus for two years plus a $5K signing bonus! More info at medschool.vanderbilt.edu/biochemistry/d…