DevermanLab

📣🎉New pooled library! This screening library from George Church's lab contains all possible single codon substitutions, deletions, and insertions in the AAV2 cap gene. hubs.la/Q022s6pR0

@Addgene Honored to have one of our plasmids posted as Addgene plasmid of the day! BI28 is a highly efficient capsid for CNS transduction in all strains of mice. It was engineered by reprogramming AAV9 to bind LY6C1 in vitro.

iCAP-BI-28-KanR Depositor: @DevermanLab Purpose: RepCap for AAV production addgene.org/203532/

@RossWilsonLab @StanleyCenter @broadinstitute @atchen_ @azari_ba @Fatma_zahra_Eid @Ayjchan Thanks Ross! I completely agree that this is a concern and a place where ML should be able to help.

@DevermanLab @StanleyCenter @broadinstitute @atchen_ @azari_ba @Fatma_zahra_Eid @Ayjchan Beautiful work - congrats to the entire team! Curious about the overlap between the "targeting" sequences and likely epitopes underlying human immune response. It seems like this is an aspect where machine learning could really help. (LMK if this is simply a non-concern!)

We have released a preprint “Targeting AAV vectors to the CNS via de novo engineered capsid-receptor interactions”. We developed gene delivery vectors with known mechanisms of action & predictable tropisms by targeting AAVs to specific proteins. tinyurl.com/2ndsuvp6

This work was made possible by our @StanleyCenter @broadinstitute team: Helen Huang @atchen_ Ken Chan Hikari Sorensen Andrew Barry @azari_ba Thomas Beddow Qingxia Zheng Binhui Zhao Isabelle Tobey @Fatma_zahra_Eid @ayjchan

We believe this strategy will accelerate the development of AAV gene delivery vectors that bind defined targets on different disease-relevant cell types with high specificity and across species to enable translation from preclinical models to investigational human gene therapies.

Early-career scientists: We want to fund your pilot studies in #genetherapy & #celltherapy! ASGCT Members can apply for one of six $100,000 #CareerDevelopment awards by Aug. 1. Learn more and apply today! bit.ly/3w8vp6m

We’re thrilled to have Andy join our team!

Come see how we are conceptually upending protein evolution approaches by building a machine learning-driven atlas to efficiently identify AAV capsids with multiple desired traits (improved tropisms + manufacturability). Talk abstract 1201 May 19, 11:45am @ASGCTherapy #ASGCT22

Ken Chan from the Deverman lab @broadinstitute @StanleyCenter is presenting data on several AAVs that can efficiently deliver genes to the brain and spinal cord. Our talk is on May 19, 10:45am @ASGCTherapy #ASGCT22 Abstract 1197