E3 Engaging Epigenetics Experts

A novel N4,N4-dimethylcytidine in the archaeal ribosome enhances hyperthermophily | PNAS pnas.org/doi/abs/10.107…

This fascinating study in Cell Reports expands our knowledge of hyper-modifications on nucleic acids and the origins of corresponding sugar-installing enzymes. @NEBiolabs is committed to advancing knowledge with basic and applied research. cell.com/cell-reports/f…

Hot off the press—the m5C epitranscriptome appears to support life at extreme temperatures. In this @NatureComms report, CSU and NEB researchers mapped 5-methylcytidine in the model hyperthermophile, T. kodakarensis. nature.com/articles/s4146…

Yuki Tanaka et al. effectively constructed an EM-seq library using crude DNA - the quickest minimal sample-loss approach for obtaining useable DNA without requiring additional dissolution and purification. doi.org/10.1016/j.bbrc… #Methylomes #NEBNext #Genomics #Epigenetics

Learn about a new method to directly detect 5hmC at single-base resolution in this interview with NEB scientist, Dan Evanich, Ph.D.. nebiolabs.com/3PbMRDg

Discovery of cytosine deaminases enables base-resolution methylome map... sciencedirect.com/science/articl…

A molecular tool specifically targeting imprintomes for systematic evaluation.....with implications for basic research, agrigenomics, and clinical practice. nature.com/articles/s4159… #epigenetics

Single-cell bisulfite-free 5mC and 5hmC sequencing with high sensitivity and scalability. #MethylationProfiling #BisulfiteFree #5mC #5hmC #SingleCell #PNAS pnas.org/doi/10.1073/pn…

Epigenetic heritability of cell plasticity drives cancer drug resistance through one-to-many genotype to phenotype mapping biorxiv.org/content/10.110…

Methyl-SNP-seq reveals dual readouts of methylome and variome at molecule resolution while enabling target enrichment... ncbi.nlm.nih.gov/pmc/articles/P…

Chun-Wei Chen, et al. SRCAP mutations drive clonal hematopoiesis through epigenetic and DNA repair dysregulation(2023) Cell Stem Cell DOI: doi.org/10.1016/j.stem… #epigenetics

Diagnostic labs use IHC as the gold standard to ID primary sites of metastatic cancers. Here, an AI molecular classifier predicts the primary site with RRBS libraries based on only 10–50 ng of DNA from formalin-fixed paraffin-embedded tissues nature.com/articles/s4146…

‼️ ONLINE NOW @NatureGenet 📰 Crosstalk between RNA m6A and DNA methylation regulates transposable element chromatin activation and cell fate in human pluripotent stem cells 🧑🏿‍🤝‍🧑🏼 @YaruiDiao , Tongyu Sun, and team 👇🏿 go.nature.com/44SPNdx

Excited to share our work in @biorxivpreprint: discovery of 175 deaminases from 13 families uncover novel properties, enables a Single-Enzyme Methylation Sequencing method allowing high coverage base resolution methylome analysis of low input @NEBiolabs biorxiv.org/content/10.110…

EM-seq - 30X methylome whole genome sequencing, low-input (down to 10 ng) alternative to bisulphite WGS. See: pubmed.ncbi.nlm.nih.gov/34140313/ /4 of 7

Good news: You can still download our @CellCellPress paper about aging and its reversal - for free. While stocks last! authors.elsevier.com/a/1gPf5L7PXir3R

Vernaz, G., Hudson, A.G., Santos, M.E. et al. Epigenetic divergence during early stages of speciation in an African crater lake cichlid fish. Nat Ecol Evol 6, 1940–1951 (2022). doi.org/10.1038/s41559…

Flanking sequence preferences of TET enzymes, DNMT3 enzymes, and DNMT1 are completely different. The concept of how their crosstalk would determine epigenome patterns is fascinating. nature.com/articles/s4200… #5hmC #5mC

University of Wisconsin study implicates #epigenetic mechanisms in the regulation of HLA-I in prostate cancer and points to epigenetic therapy as a promising option for enhancing the immune response in prostate tumors. nature.com/articles/s4200…

Methyl-SNP-seq allows detecting methylation status of any DNA even without a reference genome. biorxiv.org/content/10.110… Best, -NK & @NEBiolabs #WGBS