Sameer Aryal retweeté
Sameer Aryal
119 posts
Sameer Aryal
@_sameeraryal
Molecular mechanisms of neuropsychiatric disorders @broadinstitute.
Cambridge, MA Inscrit le Nisan 2013
823 Abonnements189 Abonnés
Sameer Aryal retweeté
Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and ... biorxiv.org/cgi/content/sh… #biorxiv_neursci
Română
Sameer Aryal retweeté
Akap11 mutations greatly increase risk for #bipolar disorder and #schizophrenia. Not much was known about this gene when we started. Here’s what we learned!
biorxiv.org/content/10.110…
Thanks to Morgan Sheng, @ajgrange, Yang Ge, Borislav Dejanovic & crew @broadinstitute
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Excited to share that our paper on cell-type specific changes in the striatal medium spiny neurons underlying the expression of RRBs in FXS mice is finally online @CellReports. Thanks to team members from @KlannLab, @CarterAG, @NicTritsch, and @BagniLab for their contribution!1/6
Klann Lab@KlannLab
Thrilled to share @fran_longo17's latest work describing cell type-specific disruption of striatal circuitry in FXS. Many thanks to our collaborators in the labs of @CarterAG, @BagniLab, & @NicTritsch - sometimes it takes a village! sciencedirect.com/science/articl…
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@fran_longo17 @CellReports @KlannLab @CarterAG @NicTritsch @BagniLab Congratulations @fran_longo17 ! Ad maiora semper!
Português
Sameer Aryal retweeté
Nat Biotech recently published a provocative study claiming single synapse RNA-seq. Unfortunately, there is no evidence demonstrating droplet-based sequencing of single synaptosomes. They are likely studying random pieces of cellular debris. Our reply:
nature.com/articles/s4158…
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Sameer Aryal retweeté
🧵4/4
@_sameeraryal shows shared molecular changes in schizophrenia and bipolar disorder, offering new possibilities for understanding and treating these disorders!#MentalHealth #Schizophrenia #BipolarDisorder 🧠💡
👉 rdcu.be/dhoxC
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The first bit of my postdoc is out now in preprint form! We were interested in the molecular mechanism behind sharp increases in protein synthesis resulting from neuronal activity. 🧵👇
Klann Lab@KlannLab
Really excited to share @Mauricio_Neuro's latest work, where he describes a novel mechanism for eIF2alpha dephosphorylation in response to plasticity-inducing stimulation, answering a long-standing question in the field of neuronal translation. Congrats! biorxiv.org/content/10.110…
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Sameer Aryal retweeté
A deep, unbiased analysis of synapse proteins in brain tissue from individuals with schizophrenia, those with bipolar disorder, and unaffected individuals uncovers molecular evidence that these disorders have more in common than previously thought. broad.io/SynapseProteom…
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Pleased to note that this study is now published in the open-access journal @CellReports!
Sameer Aryal@_sameeraryal
We carried out proteomics of synaptic fractions purified from the DLPFC of humans with schizophrenia and bipolar disorder. We found multiple interesting changes, some of which were also seen in mice lacking Akap11, a shared risk gene for both diseases. Happy to share the work!
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@PrabhatSKunwar @CellReports Thanks very much for the kind words!
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@_sameeraryal @CellReports Congratulations Sameer and the team! Foundational work!
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@prerana123 @CellReports Thank you so much! Hope all is well!
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@Mauricio_Neuro Anadolu, Sossin (2022) has sequencing data from biochemically purified mRNP granules (P5 brains). biorxiv.org/content/10.110…
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Which brings another question: any sequencing data out there from neuronal mRNP granules?
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If I wanna isolate mRNP granules from neurons, what would be the best approach? Transfect cells with GFP-Dcp1a and sort ~500 nm particles? Any other approaches I am not familiar with?
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@Mauricio_Neuro For biochemical enrichment from brain tissue, the approach described by El Fatimy (2016) and Aschrafi (2005) may be useful. See #pgen.1006192.ref018" target="_blank" rel="nofollow noopener">journals.plos.org/plosgenetics/a…
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Happy to share two recent studies I contributed to. The first led by @fran_longo17 found that cell-type-specific disruption of cortico-striatal circuitry drives repetitive patterns of behavior in fragile X syndrome model mice.
Francesco Longo@fran_longo17
Excited to share our last work from @KlannLab where we unveiled cell-type specific changes in the striatal medium spiny neurons underlying the expression of RRBs in FXS mice! A great collaboration with @CarterAG, @NicTritsch, and @BagniClaudia labs! 1/5
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@_sameeraryal Congratulations Sameer! You're on a roll. 👏
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