David Joly

Maybe a dumb question, but in a complete chromosome, is it normal to have base pairs after the telomeric repeats? Some of my T2T chromosomes sometimes have 300 bp after the repeats… Assembly artefacts?

@striiformis @Sck26C Can we still say urediniologists? Or are we pucciniologists now?

Arthur fungarium is the only specific rust collection located in West Lafayette, IN. It is the Mecca for all uredinologists around the world. The state of IN now has an updated rust checklist in celebration of the fungarium. mycokeys.pensoft.net/article/148853/

Hidden Harvest is Excited to Be Part of Groundbreaking Cannabis Research! We’re thrilled to be part of the ongoing work at Université de Moncton, where some of our Hidden Harvest genetics are playing a key role in developing PM-resistant cannabis cultivars.

@ChimeraGenetics You generated them yourself? Is it from a published protocol?

Dihaploid on the left, a haploid on the right from a different gamete. Microspore & macrosporogenesis pathways to haploids are well under way in Cannabis - she is moving out of the closet & into the lab ! The future seed biz is going to be very different

Interactions of sequence diverse effector proteins of wheat powdery mildew control recognition specificity by the corresponding ... biorxiv.org/cgi/content/sh… #biorxiv_plants

Join us for this interesting discussion on careers beyond academia. Register for the online CSPB ERM 2024 (Dec 5th and 6th). event.fourwaves.com/cspb-erm-2024/…

Never heard of a -80 that broke during regular working hours. Those things know when you’re not reachable. Still got there in time (-47) to make sure our precious pollen is kept safe. I would think it would lose viability quicker than bacterial cultures at room temperature.

@ATinyGreenCell @aourirabderahim @SaraTetraDec And if this email is what I think, this is exactly why we want you on that panel!

@aourirabderahim @SaraTetraDec Yeah im self taught. Dropped out after 3 years of undergrad for fam/financial reasons and could never find resources to go back. I do contract research now and it pays the bills, but also taking care of my elderly mom so it would be financially unwise to go back to undergrad.

Chill.

Looking for someone that still has the installation disc (or equivalent) for the Perkin Elmer 2030 Workstation… I have an old Victor X3 for which the computer stopped working and I lost the CD!

@ImperfectFunGuy @WestVirginiaU @DanielleMikola1 This will be my 12th fall semester at @umoncton, and the 10th I’m teaching Botany (with the lab). And even though my first love was also rust fungi, I’m now onto cannabis powdery mildew!

@zpagomez @richamelin @nfeau @JaniceC76821063 Same story for C. quercuum/C. harknessii !? You need to dig out the old genomic data!

Didn't make it to #IMC12 but want to learn about Cronartium pini, the fungus behind Scots pine blister rust? No worries! Here's my poster with preliminary results. If you're into rust fungi research, I'd love to discuss it further! 🌲🍄 #IMC12 #RustFungi #PlantPathology

@dromius Why not use the same colors for panel A and B !? A small detail that would make this figure much better!

Tobacco leaf single cell expression atlas with new marker genes for different cell types Feng et al. 2023 doi.org/10.1111/ppl.14…

@wheatpath @SebbyRust Cumminsiella? I just know it can infect Berberis, might look very different, I don’t know…

The severe attack on two #Berberis plants, with a distinct symptomatology compared to others, particularly dense aecia on deformed flower peduncles, casts doubt on the rust identification: is it a distinct f. sp. of #Puccinia graminis or another species? 🤔

@asc_csa @CRSNG_NSERC Au Lone Pine Cemetery… rien de mieux qu’un endroit un peu glauque pour faire ressortir l’aspect « eerie » de la noirceur qui s’installe avec le soleil en haut de nos têtes! Photos prises avec un iPhone…

La voici! La spectaculaire éclipse solaire totale du 8 avril 2024! 👏 Dites-nous où vous vous trouviez pour vivre ce moment extraordinaire. 📷: Sheetal Puri / ASC.

Ok, this was way more impressive than I was thinking (and way more than the best I could capture with my phone)… #Eclipse2024

@wheat_research @miiiike_sm Make sense! Wheat accounts for 2-3% of Canada’s GDP… But then, where is the $3.5M for a Canadian National Cannabis Cluster (cannabis = 0.5% of Canada’s total economy!).

NEWS RELEASE: @wheat_research to administer new, $20-million Canadian National Wheat Cluster Read more: ow.ly/nFFL50QN6CL #cdnag #westcdnag #wheatresearch

~20 years ago, I discovered a hybrid between two rust fungi. A paper just came out that investigated the frequency of these hybrids in WY and CO, and showed that some isolates could infect C. ribicola alternate host (Ribes spp.). @richamelin shorturl.at/hFM07

@dromius It’s a powdery mildew species ;)

@idjoly What pathogen are we talking about? You can try and 'infiltrate' a region with the R gene and another with a control so that they border on each other, then inoculate with the pathogen between the infiltrated regions and see whether the pathogen extents more into the control.

Question for the #MPMI crowd: if I have a ongoing infection on an N. benthamiana leaf, then agroinfiltrate an R gene recognizing the pathogen, will it kill the pathogen? Trigger a noticeable HR?

@dromius I think I remember that (Avrblb2 paper?). Yeah, I was thinking of having the pathogen first, then agroinfiltrate (even though it will likely affect the infection even with an empty vector). But could try both, before and after the pathogen…

@idjoly I have in the past transiently expressed a Phytophthora R gene in N. benth and then subsequently applied spores or mycelial plugs (24hpi). It was effective in limiting the spread of an infection beyond the initial inoculation site (as compared to a GFP control expression).

@dromius Thx Sebastian. It’s not much about the protection, but rather than I want to see if the R gene is working. And I would like to avoid stable transformation… (and I don’t know the Avr, yet).

@idjoly It likely depends on the pathogen and the speed of disease progression. If you have a slow and local pathogen, you might be able to protect the surrounding not-yet infected tissues by expressing an R gene which is effective against that pathogen.