Application Specialist Team

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Application Specialist Team

Application Specialist Team

@4Specialists

MYcrocopists, Non-linear enthusiasts, multiphoton-mad, brunch of neuroscienctists

Katılım Mart 2021
213 Takip Edilen88 Takipçiler
Application Specialist Team
Application Specialist Team@4Specialists·
We are glad to see an outstanding work that we have been able to contributed to 🙂 Congratulation @MichaelLinLab !
Prof. Michael Lin@MichaelLinLab

Super-excited to reveal our latest fluorescent voltage indicator, ASAP5, now online @NeuroCellPress! ASAP5 features higher responsivity and kinetics, allowing detection of single synaptic transmission events. The link gives free access until 2024.11.09: authors.elsevier.com/a/1joYd3BtfH9B…

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Tony Kelly
Tony Kelly@tonykellyEU·
preprint Alert📢 Time to talk about the🐘 in the room of genetically encoded Calcium indicators (GECIs). These indicators are widely used to report activity in the brain and an invaluable tool, but the expression of these GECIs can change activity. 🧵1/7 biorxiv.org/cgi/content/sh…
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FEMTONICS
FEMTONICS@Femtonics·
🍂 Dive into #MultiphotonImaging this autumn! Embark on a special autumn microscopy journey with Nikita and Tamás for an insightful lecture and demo. 🗓️Date: Oct 26 🕒Lecture: 15:00 GMT+2 🔬Demo: 16:00 GMT+2 Save the date! @lazy_reasonings @tamastompa @4Specialists
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Application Specialist Team
Application Specialist Team@4Specialists·
One of good solutions was the 3D real-time motion correction (RTMC), that could eliminate motion artifacts caused by running :) @Femtonics @FemtonicsUSA *See the preliminary story below.
Application Specialist Team@4Specialists

Yesterday, I was so happy to find nice neurons in the visual cortex for Ca2+ imaging, and I could selected them using chessboards in 3D...but during the measurement, the mouse started to run... I needed to find a good solution to avoid movements! (coming soon) #neuroscience #AOD

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Application Specialist Team
Application Specialist Team@4Specialists·
Yesterday, I was so happy to find nice neurons in the visual cortex for Ca2+ imaging, and I could selected them using chessboards in 3D...but during the measurement, the mouse started to run... I needed to find a good solution to avoid movements! (coming soon) #neuroscience #AOD
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Application Specialist Team
Application Specialist Team@4Specialists·
⚠️Weather update⚠️: after our observation of the hippocampal storms🌩️, our neurometeorologists started analyzing the phenomena. First, we measured the speed of the waves, which were around 24.4±3µm/s. Do you have an idea on what could spread so slowly? #hippocampus #neuroscience
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Shanshan (Amber) Han
Shanshan (Amber) Han@amberHan99·
@4Specialists I saw this wave too, using UCLA miniscope and the same virus in the hippocampus, the mice were freely moving, on the linear track. At first, I thought this wave related to the behavior, but it was not. So I don’t know how to explain it.😭
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Application Specialist Team
Application Specialist Team@4Specialists·
Some details: Video was taken by 2p acousto-optical microscope. Scanning freq ~40 Hz, on 500x500 um. AAV GCamp6f was injetced in the HC of mouse. During experiment the headfixed mouse was running in a gramophone.
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Application Specialist Team
Application Specialist Team@4Specialists·
@Noni_sdh Thanks for the comment! It looks overexpressed indeed. However we have never seen similar slow waves before! It was recorded by 2p AO microscope from @Femtonics.
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Noa Sadeh
Noa Sadeh@Noni_sdh·
@4Specialists Overexpression of GCaMP. Try to dilute the virus and lower the LED power. If you are working with these mice for a long period, you need to find the minimal dilution that produces a decent fluorescent signal after ~4 weeks.
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Keşfet

@MichaelLinLab @tonykellyEU @UniBonn @Columbia_NTC @UZH_ch @researchsquare @lazy_reasonings @tamastompa