Adam

What ties all the Polybio presentations together is a strong emphasis on the persistence of SARS2 - often in a replication-competent form. This persistence is primarily located in tissues, especially in the gastrointestinal tract (ileum, lamina propria, sigmoid colon). Nicolas Huot Found replication-competent SARS2 in the ileum of primates, localized near lymphoid structures. Marcus Buggert Detected persistent viral RNA in the gut along with increased interferon signaling. Tim Henrich Showed persistence in the lamina propria and associated changes in immune cells. Eduardo Reategui Presented the BARA platform (using extracellular vesicles) with very strong detection accuracy. Van Brocklin PET scanning to identify areas of T cell activation across patient’s whole bodies These studies strongly suggest functional parallels with HIV - Viral reservoirs in immune cells and GALT Chronic T-cell activation and exhaustion Long term immune dysregulation driven by persistent viral antigens. PolyBio is approaching Long COVID as a chronic infectious disease with a viral reservoir, rather than merely a post-viral syndrome. The transfer of knowledge and experience from HIV research is one of the strongest accelerators of progress in this field!

Denmark, good job! Link in comments. @KevinMcCairnPhD @CharlesRixey @HouseLyndseyRN

@_VFK_DAA Absolutely terrifying data

Meeting criteria vs biology: All Long Covid patients here met MECFS criteria. Yet 14.5% (10) are positive for brain-derived pTau-217, compared to just 1 single person in pre-2018 MECFS (3.8%) and 1 in healthy controls (2%). Similar symptoms, distinct biology. Nothing new?

A recent study in Nature Medicine shows that pTau-217 precedes Alzheimer's disease by 15 to 20 years. We need more research but based on this, it looks like we should expect an enormous wave of Alzheimer's disease beginning in roughly 10 years nature.com/articles/s4159…

Emoji summary of what was interesting to me 🩸 Across 6 papers, microclots emerged as real, measurable and potentially reversible, offering a biomarker for Long COVID treatment trials. 🦠 SARS-CoV-2 signals in intestinal cells suggest persistent viral material may help drive ongoing gut immune disruption in Long COVID. 🧬 Gut organoids reveal how genetic variants may disrupt intestinal barrier and immune function. 💊 Maraviroc plus pravastatin improved gut symptoms in early findings, while larazotide was linked to improved fatigue, gut and circulation symptoms. Both need further trials. 🐒 In monkeys, the ileum may harbor persistent SARS-CoV-2 alongside ongoing immune activation. 🐈 In feline infectious peritonitis, immune cells may help harbor and spread coronavirus. 🛡️ In Long COVID, at least 40% showed T-cell evidence of possible persistent viral material, while virus specific T-cells also showed stronger cell killing signals. 💦 Microfluidic testing detected SARS-CoV-2 RNA in some people with Long COVID and after recovery. 🔄 In 19 adults with Long COVID, MENSA biomarker status shifted over 180 days during an ongoing antiviral feasibility trial. 🧪 VIPER is testing 6 assay programs and plans to analyze more than 440 samples from blood, fluids and gut tissue to investigate viral persistence. 🫀 SARS-CoV-2 RNA in artery plaques may help sustain inflammation and worsen atherosclerosis. 📸 PET imaging may help track tissue immune activation and treatment response in Long COVID. 🦠 In chronic infection models, macrophages and other myeloid cells in the intestine and lung may harbor virus after acute infection. Depleting these cells cleared viral reservoirs. 🧠 In CCI patients, disrupted cerebrospinal fluid flow may impair clearance of metabolites and chronic antigen, potentially fueling brain inflammation and neurological symptoms.

@norhinosn16 How are you doing now Tamara? Thank you for leading the charge as patient number two!

He literally told me since I wasn’t 75 I could live longer not knowing, not having diagnoses of Amyloidosis even though MAYO says earlier is better which is correct. They poisoned us with virus and vaccine and when we try to figure out what happened. They don’t want us to know!

Trending yet again

Nicolas Huot, PhD 12:00 - 12:10 pm ET (4:00 - 4:10 pm UTC) SARS-CoV-2 Persistence in the Intestine: Innate Immune Control of Gut Viral Reservoirs Dr. Huot will present work using a nonhuman primate model to investigate SARS-CoV-2 persistence within the intestinal tract. The talk will focus on how innate immune interactions— particularly involving natural killer cells and macrophages-contribute to the containment or maintenance of viral reservoirs in gut tissues. By defining immune-viral dynamics in the intestinal microenvironment, this work provides insight into mechanisms of long-term viral persistence and their potential relevance to Long COVID pathogenesis.

1:40 pm ET- Mark Painter presented new findings suggesting Long COVID patients’ T cells have more exhaustion markers & produce reduced interferon-gamma/TNF than recovered convalescents. More work is needed to understand the key steps to either a) connect exhausted T cells to Long COVID pathology or 2) re-invigorate the exhausted cells to help improve disease outcomes.

Cure ME/CFS and long COVID. Tens of millions of people are lying in dark rooms right now, waiting for someone to crack a biology that medicine still barely understands. With the right data, if AI can decode the mitochondrial dysfunction, the immune dysregulation, the broken energy metabolism, the autonomic collapse, it will have done something no institution has bothered to fund properly in years. That would be historic. Please save us.

@evanfgeorge Thank you for sharing such detailed updates on what is an incredibly personal journey. Your attitude and positivity despite what you’ve been going through for so long are amazing. Wishing you the best possible outcome from your time in Japan.

This past weekend, I received an SGF injection into my lumbar spine from Dr. Kato. After reviewing my history and the numerous surgeries that have provided no meaningful benefit, he suggested that I consider a spinal cord stimulator. We hoped that the SGF injection in this area might provide some indication as to whether this type of intervention could be useful. After this injection it was recommended that I test myself a bit more to see how symptoms respond. Since coming to Japan, I have been walking more than I was previously. I had more or less stopped walking except around the house because it would consistently lead to neuropathic pain in my legs, feet and lower back. I think leaving an environment that had become associated with illness and dysfunction has been immensely healing in and of itself. I was steadily increasing my steps- starting at about 3,000 daily up to 6,000 without flaring and I believe the treatment was helping with this. After this localized SGF injection I tested two important symptoms. I sat for maybe 20-30 minutes on a very cozy sofa. My pain gradually built in the same way, pressure in the pelvis, pain in the scrotum, lower back pain and for me it was very interesting to observe a symptom that I have experienced before but not as clearly: As I remained seated, I became progressively more drowsy and fatigued. The longer I sat, the more tired I felt, while at the same time the pain and pressure sensations in the lower back and pelvic area continued to build. As soon as I stood up my energy came back and I felt normal. The day after this I tested myself by walking over 10,000 steps for the first time in 3 years. I felt more strength in my legs which I believe is due to the treatment so I felt confident walking this distance. That night I was fine however the next morning I woke up feeling very sore, stiff and with considerable aching pain coming from my lower back. Then in the days since it has shifted to more neuropathic pain and even standing I am feeling constant pins and needles, also my toes are going numb. It was interesting to observe how the pain and soreness were markedly worse on the right side of the body. I also consistently experience symptoms in my upper back and arms that are triggered by use. For example, typing on a computer. Since arriving in Japan, I have been sending more emails and spending more time on the computer, and that has led to increased burning sensations in my forearms, tingling in my hands, aching in my hands and elbow joints, pain in my shoulders, and discomfort around the shoulder blades. What I find interesting is how interconnected these symptoms are. For example, while in a flare, simply using my right arm will not only trigger symptoms in the arm itself, but also pain further down on the right side of my body, such as in the right groin or the right lower back. This is all to say that I am in quite a lot of pain right now and have lost some of the progress that I felt I was making. At the same time, I feel confident that I am in the right place. I trust the people here and believe in this treatment. I am seeing with my own eyes the effect it is having on some patients and watching people improve, which gives me hope. My focus right now is trying to better understand the disease process that has led to the systemic nature of this illness and why it continues to progress. I am hesitant about a spinal cord stimulator because I do not feel confident placing hardware into my lower lumbar spine while there still appears to be an active disease process occurring in that region. This is an area where diseased tissue has already been identified, where arachnoid adhesions have been observed, where the surgical wound previously dehisced, and where there are multiple signs that something abnormal has been ongoing for quite some time. I would say that the most encouraging sign so far has actually been an improvement in my sunlight sensitivity. It has not disappeared completely, but I was recently able to spend around fifteen minutes walking in direct sunlight without developing a rash. For me, that was a very meaningful sign of improvement and one of the clearest positive changes I have noticed thus far. For anyone who is currently struggling with illness and looking to the treatment here, I am learning not to compare my healing with others so much. Every case is different, every body responds differently, and not everyone will see quick improvement. I think that is especially true in more complex or idiopathic cases where significant tissue injury or nerve damage has likely already occurred. I also want to send everyone here my warmest wishes and deepest gratitude for all of the prayers, encouragement, and support. Thank you <3

@BowTiedLoon i am in the successful Anktiva long covid clinical trial. guess what: it’s extremely effective. the doctors are beaming about what they’re going to be able to publish in the medical journals about what they have been able to heal, with long covid and immunotherapy. you are stupid

Patient #12 update, 1 month out

If folk are interested, I am giving a @BiochemSoc talk on our work with @resiapretorius on Fibrinaloid Microclot Complexes in Long Covid and ME/CFS on June 17 at 2pm. Registration needed but free register.gotowebinar.com/register/22648… #TeamClots #pwLC #pwME

@CoyoteSanctuary @CumberpatchM Just donated! Use it how you see fit, team.

@CumberpatchM donate.stripe.com/cN2aF6a7leQh6y… I'm leaning this direction: Using the Message Box to Specify Donations for Doctor McCairn's Therapy Bypass every third party hosting site and just use what's existing.

@CumberpatchM Try Pateron ? Payout schedule would be the point of contention, without shutting down the Donations before the goal ?

@Evie707656912 @CoyoteSanctuary @KevinMcCairnPhD Just donated! Use it how you see fit team

This is the link to donate to @KevinMcCairnPhD so he can undergo the plasmapheresis treatment in Japan as he is Covid-19 virus injured . He is responsible for founding this treatment protocol for the jab and virus injured. He is vital for the research he has been doing, so getting him healthier is a priority. Please donate to fund his medical expenses for the treatment protocols and RT this liberally.

A message for Doctor Kato and all the wonderful people in Japan. This one got emotional. I had to hide in the front of the boat to keep my friends from noticing. I hope my friends in Japan see this.

And filtration #2 for me Patients #17 and 18 finishing up! as #'s 19-23 keep going and 24-28 starting soon

@robert65968 @kelseyshields08 Thank you for keeping us all up-to-date Robert! Eager to see you keep improving, but even being able to be out on the boat again with your buddies must feel like heaven

One month post treatment in Japan. Short update. Still doing well. Next update in a few weeks focusing on post treatment care in USA.