Dr. Beeke Wienert

@doyonlab Adding a furin cleavage site in addition to 2A can help with efficiency and also shorten the peptide scar!

@grunewaju @TU_Muenchen @dfg_public @JKeithJoung @TumTransla Congratulations, Julian!! Can’t wait to hear more!

Extremely excited to kickstart my lab @TU_Muenchen with Emmy Noether support from @dfg_public. I’m very grateful to @JKeithJoung, my friends in Boston, and to A. Moretti, K.L. Laugwitz & C. Kupatt in Munich. More details & website coming soon. #CRISPR 🫀

@KateGQuinlan @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts 💯

@BeekeWienert @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts Can I steal that quote?! I’ve got a talk I’m giving on Friday which fits that very well!

I’m absolutely delighted that @MerlinCrossley & I are finalists for the 2022 Eureka Prize for Scientific Research! We’re nominated for our work using gene editing in blood cells, conducted by wonderful @unswbabs staff & students, past & present @unsw @UNSWScience @eurekaprizes

@KateGQuinlan @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts CRISPR made everything happen! 🙏

@BeekeWienert @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts Thank you, Beeke! This was all kicked off by your PhD project 😊

@KateGQuinlan @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts Congrats Kate and Merlin!! 🔥🔥

Recent lab members who have contributed to this work include: @HenryWBell @sonia_goozee @LanaCLy @manan_s9 @gabmartyn @ethbey @BeekeWienert @LuYang_bio @SarahTopfer_ @lo_sullivan @leesa_lerts & twitterless Ali Funnell, Taya Michel, Claire Milthorpe, Laura Norton & Richard Pearson

@Nicole_Paulk Seriously. Buying that 1M Tris-HCl at ph 7.4 has changed my life 🤌🏼

@BeekeWienert You got dat big money 🤑

Calling #scitwitter #biotwitter #chemtwitter does anyone have a pH meter they actually like? Easy to use, easy to clean, accurate, not fussy, simple user interface, lasts longer than 1 year before croaking? Please comment below! 🙏

Anyone I know attending #EHA2022 ?Come say hi to me at my poster 1436 representing the @GraphiteBio Team tomorrow afternoon!

Come join my team at @GraphiteBio to help develop next generation gene therapies for patients in need and have a lot of fun along the way. Mammalian Cell Bio is a must, CRISPR is a plus! Pls retweet! Scientist I/II, Next-Gen Editing linkedin.com/jobs/view/2967…

@richardson_lab Good Q. I think DNA triggers the Sting pathway so would be interesting to see if there’s a differential response, but yes also number of edited cells would be a readout

@BeekeWienert We think we can make this less toxic than uncrosslinked templates because we can use less of it. It also works with things that suppress toxicity (ssDNA, etc). You know more about this than we do, so what’s the best thing to show? Total edited cells X hours after treatment?

Take a look at this! We show that adding interstrand crosslinks to DNA used as a template for homology directed repair boosts the frequency of gene editing. biorxiv.org/content/10.110…

Great to have contributed to this paper on using #CRISPR base editing to boost fetal hemoglobin to treat #SickleCellDisease led by Dr Mohankumar in India and now published in @eLife - CRISPR base editors are already the next big thing - thanks @davidrliu doi.org/10.7554/eLife.…

@bsadamson Where you going, Britt? 🙌

Looks like I am going to get to fly again. First time in 2 years. What do I need to know? Is pre-check still a thing?

@jcornlab @amosliang2 @dundeeuni @mrcppu Oh hell yes, @amosliang2 !! Congratulations!

Fabulous postdoc @amosliang2 will be joining @dundeeuni @mrcppu this year as an Asst Prof. Continuing his ground-breaking work on #ERphagy, #CRISPR, and more. Keep an eye out for big things from his new lab!

@Chemjobber Liholiho Yacht club!! 🙌

Bay Area friends, what's a great restaurant to get a gift card to? Looking for something in the $100-$150 range

@ajknights7 @embojournal @UMLifeSciences Cool stuff, Alex!! Looks amazing!!

Pleased to share our cover art for EMBO! Our discovery of cholinergic adipose macrophages activating thermogenesis brought to mind the Greek myth of Prometheus, who brought fire to humanity. Grateful to the EMBO team who helped turn my concept into beautiful Hellenic-inspired art

Today, we announced that the first patient has been enrolled in our Phase 1/2 clinical trial of GPH101, an investigational gene-edited autologous hematopoietic stem cell therapy designed to directly correct the genetic mutation that causes #SickleCellDisease. #SCD

@NucleaseLab @ExcisionBio Congrats!!!

.@ExcisionBio announced FDA clearance of our IND for Phase 1/2 of EBT-101 CRISPR-based therapeutics for treatment of HIV We look forward to initiating our Phase 1/2 trial at the end of the year. #CRISPR targeting #HIV

Senior Scientist Beeke Wienert loves working with 🧬 #CRISPR technologies to improve the precision of #geneediting therapies and pioneer a novel approach to curing diseases for patients. Read about the adventure that brought her to #GraphiteBio.

@richardson_lab Woooooooohooooo! Amazing! Congrats, Chris!

The lab is now NIH funded! Many thanks to mentors and students for making this possible. Excited (and a little nervous) to see where these projects take us.