Deepshika Pulimamidi

@STOPlabPI @AgataKStepien @real_lab_duck @kopczynska_m And also many thanks to @michal_gdula and our collaborators Maxime Lalonde, Shazia Irshad, Hiroshi Kimura, and Stephan Hamperl

Check out the full story in our lab's latest pre-print biorxiv.org/content/10.648… Huge thanks to our lab @STOPlabPI @AgataKStepien @real_lab_duck @kopczynska_m for their joint efforts on this project

Ever thought the two closely related transcription processes, pre-mRNA 3'cleavage and RNAPII termination, are always coordinated? Turns out, these two events can sometimes go in opposite directions, and their proximity is linked to increased gene expression!

🚨New preprint from our labs (STOP lab & Nojima lab @pol2rna)! @kopczynska_m and Chihiro Nakayama have been digging into how chromatin remodeller SETD2 controls the start and end of transcription 👇 (1/5) biorxiv.org/cgi/content/sh…

📢 𝗞𝗶𝗻𝗴𝗮 𝗞𝗮𝗺𝗶𝗲𝗻𝗶𝗮𝗿𝘇-𝗚𝗱𝘂𝗹𝗮 @STOPlabPI, 𝗣𝗿𝗼𝗳𝗲𝘀𝘀𝗼𝗿 𝗮𝘁 @UAM_Poznan, 𝗵𝗮𝘀 𝗯𝗲𝗲𝗻 𝗮𝘄𝗮𝗿𝗱𝗲𝗱 𝗮 𝗣𝗿𝗼𝗼𝗳 𝗼𝗳 𝗖𝗼𝗻𝗰𝗲𝗽𝘁 𝗴𝗿𝗮𝗻𝘁 𝗳𝗿𝗼𝗺 𝘁𝗵𝗲 @ERC_Research! She leads the Genome Regulation Research Group at the @czt_uam and the @UAM_IBMiB. Together with Dr. Martyna Plens-Gałąska @real_lab_duck, she developed a modern methodology that forms the basis of the awarded grant. 🧐 The project, titled 𝗜𝗺𝗽𝗿𝗼𝘃𝗶𝗻𝗴 𝗰𝗮𝗻𝗰𝗲𝗿 𝘁𝗵𝗲𝗿𝗮𝗽𝘆 𝗯𝘆 𝗶𝗱𝗲𝗻𝘁𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗻𝗼𝘃𝗲𝗹 𝗱𝗿𝘂𝗴 𝗹𝗲𝗮𝗱𝘀 𝗺𝗼𝗱𝘂𝗹𝗮𝘁𝗶𝗻𝗴 𝘁𝗿𝗮𝗻𝘀𝗰𝗿𝗶𝗽𝘁𝗶𝗼𝗻 𝘁𝗲𝗿𝗺𝗶𝗻𝗮𝘁𝗶𝗼𝗻 𝗯𝗿𝗶𝗱𝗴𝗶𝗻𝗴 𝗺𝗼𝗹𝗲𝗰𝘂𝗹𝗮𝗿 𝗯𝗶𝗼𝗹𝗼𝗴𝘆 𝗮𝗻𝗱 𝘁𝗵𝗲𝗿𝗮𝗽𝗲𝘂𝘁𝗶𝗰 𝗮𝗽𝗽𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻𝘀 has been awarded €150,000 for 18 months. 🧫 Cancer is one of the leading causes of death in Europe, affecting millions of lives. It develops when cells in the body start dividing uncontrollably, sometimes spreading to other parts of the body. Cancer progression is a complex process that disrupts numerous molecular pathways, which can vary depending on the type and location of the cancer. Current cancer therapies often target only a few specific molecular pathways, enabling pharmaceutical companies to develop drugs more quickly and effectively. However, this approach does not always address the complexity of the disease—cancer cells can exhibit diversity and adapt and become resistant to treatments over time. 🧬 Despite these challenges, cancers have weaknesses. Recent discoveries have shown that the Achilles' heel of cancer cells lies in the final stage of transcribing genetic information from DNA to RNA. Most human genes have multiple alternative endpoints, and selecting the correct endpoint can influence the final product, the protein. To harness this knowledge for potential cancer therapies, Prof. Kinga Kamieniarz-Gdula and Dr. Martyna Plens-Gałąska developed an innovative method for identifying new drugs to direct where a gene ends. 👩🏻‍🔬👩🏻‍🔬 The researchers’ strategy is unique because it enables high-throughput and direct monitoring of this process. Under the Proof of Concept grant awarded to Prof. Kamieniarz-Gdula by the ERC, the researchers plan to screen thousands of potential drugs and further refine their methodology. Ultimately, they aim to discover new and more effective therapies for cancer patients.

What a start to 2025! @ApoorvaShr65367 - first XY joined STOP lab, T4ph paper published @NAR_Open, @AgataKStepien gave a well-received invited talk @igcpan, student Yuliia got fellowship from @EMBO, new grant awarded (stay tuned for official info) & 3 cool new manuscripts cooking

Do you know there is a convenient marker for human/animal #gene ends and #transcription #termination? RNA Pol II CTD T4ph. Find our all about it in our paper @NAR_Open by @kopczynska_m @upasanasaha11 et al. academic.oup.com/nar/advance-ar… Grateful to @NCN_PL and @ERC_Research for funding

Looking for an exciting #PhD project in #molecularBiology? How about this question: what determines a gene's end? Most human genes have alternative ends, the choice of which is important for health and disease. Yet we still don't understand how this choice is made!

#RNA24 went wild! Thank you @lexogen! @STOPlabPI @real_lab_duck @upasanasaha11 @DPulimamidi & Ewa

STOP lab team from Poznan/PL arrived in lovely May Edinburgh & looking forward to #RNA24 @RNASociety @upasanasaha11 @DPulimamidi @kwiatkowska_jk @real_lab_duck & Ewa

There is no border for science. Let's support each other. Empower Dreams: Bioinformatics Scholarship Fund for Ukrainian Scholars (please donate and RT) betterunite.com/bioinformatics… @BioinforUA @serghei_mangul @STOPlabPI @michal_gdula @rafalab @wolfgangkhuber

#NCNtoTlen Research at our Institute of Molecular Biology and Biotechnology @UAM_IBMiB @UAM_Poznan is funded in 90% by competitive grants from @NCN_PL. Most of the PIs recruited came to us from abroad, including Polish, but also Indian, Czech and Dutch group leaders: 1/2

Bioinformagicians at work in STOP lab :) @kopczynska_m @DPulimamidi and our dear guest @mille_qian_sen from @pol2rna lab

Dear MolBiol Twitter, any advice? Aim: separation-of-function experiments in human cells. Approach: depletion of endog. protein with dTag + rescue with dox-inducible wt and mutant stably integrated in the FlipIN system. Generated cell lines with the degron and OE, dTag works,