Dorothy A Wheatcraft

2K posts

Dorothy A Wheatcraft banner
Dorothy A Wheatcraft

Dorothy A Wheatcraft

@DRAWheatcraft

Formerly hiding in Maine, the siren song of Maryland calls to this proteomics (and more) mass spectrometrist. @[email protected]

Gaithersburg, MD Katılım Haziran 2011
1.8K Takip Edilen882 Takipçiler
Stacy Malaker
Stacy Malaker@StacyMalaker·
Congrats to @VRAdoesMS and @joann_chong!! 🥳🥂The paper is officially online now @J_ASMS: pubs.acs.org/doi/10.1021/ja… Also, check out this really awesome cover art 😍
Stacy Malaker tweet media
Stacy Malaker@StacyMalaker

Hey there, O-glyco enthusiasts! Do you use PNGaseF before your glycoproteomics runs? If so, you might want to check for "free" N-glycans hanging around in your sample. Check out @VRAdoesMS and @joann_chong's new preprint: 10.26434/chemrxiv-2024-568nn

English
1
7
30
2.6K
Joao Pereira
Joao Pereira@jdpereira·
A friendly reminder, that no matter how you feel today, happy, sad, hopeful, disappointed, you are NOT ALONE. That is all. You are loved. And you know what? It’s ok not to get science done today.
GIF
English
1
3
38
2.1K
Dorothy A Wheatcraft retweetledi
Susie Dent
Susie Dent@susie_dent·
Word of the day is ‘recrudescence’ (17th century): the return of something terrible after a time of reprieve.
English
2.8K
39.5K
193.9K
6.9M
Dorothy A Wheatcraft
Dorothy A Wheatcraft@DRAWheatcraft·
@kapahi_pankaj @J_my_sci I love laying out two carefully selected choices for 4 to reject and pick something random from the closet or drawer. But at least it speeds up the process
English
0
0
1
23
Pankaj kapahi
Pankaj kapahi@kapahi_pankaj·
@J_my_sci Yes my younger daughter used to take 20 minutes at least to choose her clothes from age 4-7 - now she is much faster … But it’s an art form choosing her clothes and she is much better than all of us
English
1
0
1
71
Dorothy A Wheatcraft
Dorothy A Wheatcraft@DRAWheatcraft·
@AaronStorey4 Very much so. I feel like this area isn't discussed much at all. It would be a great topic to get insight as to how others have learned from mistakes and moved forward.
English
1
0
1
52
Aaron Storey
Aaron Storey@AaronStorey4·
@DRAWheatcraft Sure: trying new things in a way that you can afford to make mistakes and learn from them. Easier said than done though.
English
1
0
1
58
Dorothy A Wheatcraft
Dorothy A Wheatcraft@DRAWheatcraft·
Not sure if the best forum, but informal survey #TeamMassSpec Is there a trait that you've developed that has been critical to your success? One under-developed for you that you think is critical? Also if you want, PM me if you are willing to give me feedback for myself.
English
2
0
1
440
DavidGZ
DavidGZ@DavidGomezZep·
We are excited to present MAETi, a simple and antibody-free workflow for #immunopeptidomics of minute samples, down to 50k cells. @JulianBeyrle has done great work optimizing and thoroughly testing it. Congrats on the excellent poster presentation and travel award at #HUPO2024!
Julian Beyrle@JulianBeyrle

Thrilled to have shared our latest research on "MAETi: Mild Acid Elution in a Tip enables MS-based immunopeptidomics profiling from as low as 50,000 cells" at #HUPO2024 in Dresden! (1/3)

English
2
1
13
874
Dorothy A Wheatcraft
Dorothy A Wheatcraft@DRAWheatcraft·
@UCDProteomics I have a project where it is unavoidable-can chat offline. But for 90% of the time, I agree there are other approaches.
English
0
0
1
75
Brett Phinney
Brett Phinney@UCDProteomics·
Happy to hear I'm wrong, but man I would not spend my energy on SILAC labeling these days compared to the alternatives. Even MS1 LFQ DDA with MBR on non silac samples seems better to me. that works a lot better now than it used to.
Davis, CA 🇺🇸 English
1
0
2
786
Brett Phinney
Brett Phinney@UCDProteomics·
As I go through a recent SILAC experiment I'm becoming convinced it's a relic that should probably be discarded.
Davis, CA 🇺🇸 English
1
0
11
1.8K
Dorothy A Wheatcraft
Dorothy A Wheatcraft@DRAWheatcraft·
I call this 'fixing the LC' when 4yo wants to dress up the digital birb like Mom.
Dorothy A Wheatcraft tweet media
English
0
0
1
135
Dorothy A Wheatcraft retweetledi
Julia Laskin Lab
Julia Laskin Lab@LabLaskin·
Please, RT to help us spread the word. The annual CAID Workshop (chemical instrumentation) will be held on Nov 6-8, 2024. It is a great opportunity for students and researchers at any level to learn about analytical instrumentation through hands-on demos: purdue.edu/discoverypark/…
English
0
20
23
2.8K
Fengchao Yu
Fengchao Yu@fcyucn·
@DRAWheatcraft @UCDProteomics You might be getting the errors that I am getting 😅. We are working with the Skyline team to make the process more robust and smooth.
English
1
0
3
133
Brett Phinney
Brett Phinney@UCDProteomics·
I almost never analyze SILAC samples anymore. Is it becoming less popular? Anyway I was really impressed with using the latest version of Fragpipe -w/ skyline integration for dda and Spectronaut 19 for DIA.
Brett Phinney tweet mediaBrett Phinney tweet media
Davis, CA 🇺🇸 English
4
0
26
2.7K