Deep Seq (@DeepSeqNotts) - Twitter Profili | Zamantika Mersobahis Locabet

Sabitlenmiş Tweet

Deep Seq@DeepSeqNotts·20 Ara

Hey @nanopore we have a weird blocking pattern on our flowcell... can you help?

English

3

101

316

0

Deep Seq@DeepSeqNotts·22 Haz

Just a few flowcells to return to @nanopore

English

2

0

20

1.5K

Deep Seq@DeepSeqNotts·14 Tem

We have an opportunity for someone to join our fabulous bioinformatics team - nottingham.ac.uk/jobs/currentva… - you will get to work with @nanopore @illumina @bionanogenomics @10xGenomics and more! Please retweet and dm for info!

English

0

17

13

0

Deep Seq retweetledi

Michael Hubank@generoom·30 Mar

Rapid #genome-wide copy number profiling using adaptive sampling on @nanopore by @mattloose and the amazing team @DeepSeqNotts Real potential for clinical application.

Matt Loose@mattloose

This gif is from one sample of three run on a single @nanopore promethION flow cell. Frankly I'm super impressed at the performance though there is headroom for more improvements. We ran the same libraries as on gridION and have updated our preprint here. biorxiv.org/content/10.110…

English

0

2

5

0

Deep Seq retweetledi

Matt Loose@mattloose·30 Mar

This gif is from one sample of three run on a single @nanopore promethION flow cell. Frankly I'm super impressed at the performance though there is headroom for more improvements. We ran the same libraries as on gridION and have updated our preprint here. biorxiv.org/content/10.110…

GIF

English

2

14

53

0

Deep Seq@DeepSeqNotts·22 Ara

Happy Christmas all!

Deep Seq@DeepSeqNotts

Hey @nanopore we have a weird blocking pattern on our flowcell... can you help?

English

0

1

7

0

Deep Seq retweetledi

Matt Loose@mattloose·2 Ara

We've put out a short preprint today showing some updates in Readfish for @nanopore adaptive sampling. biorxiv.org/content/10.110… @alexomics is giving a talk shortly at the #nanoporeconf about this - but a short thread here.

GIF

English

3

45

128

0

Deep Seq retweetledi

Matt Loose@mattloose·17 Eyl

Earlier this week we put out a couple of preprints (doi.org/10.1101/2021.0… doi.org/10.1101/2021.0…) describing minoTour github.com/LooseLab/minot…, an @nanopore tool we have been developing for several years. A bit of a 🧵

English

5

34

89

0

Deep Seq retweetledi

Matt Loose@mattloose·1 Şub

I still remember the first time I saw data from @scalene showing ultra long reads (N50>=100kb) were possible. Since then we and others (especially @DrT1973) have worked to get longer reads. This weekend we tested the @circulomics @nanopore ultra long kits. Wow! 19.49 Gb N50 143kb

English

4

30

95

0

Deep Seq@DeepSeqNotts·23 Ara

Well @nanopore - always seems to happen this time of year... bit of a bug in readfish but sure we will sort it out soon....

English

2

30

95

0

Deep Seq retweetledi

Matt Loose@mattloose·2 Ara

So - our ReadFish paper came out on Monday nature.com/articles/s4158… using GPU - but what if you only have a laptop? Here's an update from @alexomics to our tools that enable ReadFish on Mac, Linux or Windows with no additional GPU or compute. looselab.github.io/2020/12/02/rea… for details.

English

4

36

107

0

Deep Seq@DeepSeqNotts·2 Ara

One for #nanoporeconf

Matt Loose@mattloose

So - our ReadFish paper came out on Monday nature.com/articles/s4158… using GPU - but what if you only have a laptop? Here's an update from @alexomics to our tools that enable ReadFish on Mac, Linux or Windows with no additional GPU or compute. looselab.github.io/2020/12/02/rea… for details.

English

0

Deep Seq retweetledi

John Tyson@DrT1973·30 Kas

Many application spin out of this on the fly selective sequencing. Tunable custom target panels to genome gap filling..... things get very interesting from here.

Matt Loose@mattloose

Great to see this paper out - loads of hard work from @alexomics and many others to get this completed - adaptive sampling on @nanopore sequencers targeting hundreds of genes to depths in excess of 40x on a single flowcell. nature.com/articles/s4158…

English

0

2

6

0

Deep Seq retweetledi

Matt Loose@mattloose·30 Kas

Great to see this paper out - loads of hard work from @alexomics and many others to get this completed - adaptive sampling on @nanopore sequencers targeting hundreds of genes to depths in excess of 40x on a single flowcell. nature.com/articles/s4158…

English

12

84

211

0

Deep Seq retweetledi

10x Genomics@10xGenomics·16 Kas

Announcing the Visium Spatial Gene Expression for FFPE Scientific Challenge! Share how you’d use Visium Spatial to unlock the full story of your FFPE tissue samples to win an experiment performed by us. Enter by 12/23. Learn more: bit.ly/2IIPjlk #spatial

English

0

9

27

0

Deep Seq retweetledi

Matt Loose@mattloose·9 Eki

About to start out 100th @nanopore run for covid samples with the team from @DeepSeqNotts . A sad milestone but amazing work by our local team including many from @TeamNUH and @PostdocPat all in support of the work of @CovidGenomicsUK .

English

0

7

45

0

Deep Seq@DeepSeqNotts·10 Eyl

@kirk3gaard @nanopore We recommend this for samples of “unknown origin”. Let’s say someone wants to run a large batch of samples that they’ve extracted. A check on a Flongle (or minion flowcell with a flush) can quickly reveal sample problems. Good sample in - great data out.

English

0

1

3

0

Rasmus Kirkegaard@kirk3gaard·10 Eyl

Anyone doing routine @nanopore library checks on flongle flowcells before loading the samples on MinION or Promethion flowcells? Or do you just run the library directly and see what happens+nuclease flush if things look bad?

English

5

1

4

0

Deep Seq retweetledi

Gemma Clark@ViroGem·3 Tem

Massive achievement for @TeamNUH Microbiology lab, one of the first labs in the UK to obtain UKAS accreditation for SARS-CoV-2 PCR. A true testament to the hard work & dedication of the micro team, maintaining <24-hr TAT & high quality, end to end service for our patients.