Jono Abshier

173 posts

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Jono Abshier

Jono Abshier

@ExtremeGermJono

PhD Student | Center for Life in Extreme Environments 🧬 | PDX | Extreme Scientist - in training 🧫🦠 | he/him/his

Katılım Şubat 2022
212 Takip Edilen67 Takipçiler
Jono Abshier retweetledi
Rafa Pinilla-Redondo
Rafa Pinilla-Redondo@rafapinilla92·
When experiments don’t work... build a phage costume! 🎃 Happy PHAGEOWEEN!!! 👻🧬#Teamwork
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Jonathan Jacobs 🧬🦠🕹️🏕️
I just uploaded another project dataset (an RNAseq study) to SRA, BioSample, and BioProject - and along the way created step by step slide deck with screenshots of the process (web page captures and metadata tables, etc). Let me know if you're interested and I'll be happy to share it . I remember early in my career uploading to NCBI correctly was a dark art (most people skip steps or don't bother with all the metadata, used off brand annotations, or just read "optional" as "skip it" - which sadly often makes data reuse a massive hurdle later on), so.... I'm training my team later this week on how to do it "right" (at least IMHO the right way). The deck is ~47 slides... so... maybe it will be helpful to someone else too? I'm getting feedback from the team on the deck later this week. once I've updated it - I'll share it if anyone is interested. probably will throw it over onto Git or gDrive or something.
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Jono Abshier
Jono Abshier@ExtremeGermJono·
@AntoBeck @ATinyGreenCell I make sure to say PCR reactions all the time because I like the reaction I get from people who hate it 😌
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Anthony Beckhouse
Anthony Beckhouse@AntoBeck·
@ATinyGreenCell oh no you didn't just write "PCR reactions"..... it's like fingernails right down a blackboard.
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Sebastian S. Cocioba🪄🌷
Sebastian S. Cocioba🪄🌷@ATinyGreenCell·
Do y'all do this too when you wanna save tubes but still run a small sample of your PCR reactions on a gel before purifying? 2.5uL Purple Loading Dye 10uL PCR Sample Pipette up and down in puddle and load immediately.
Sebastian S. Cocioba🪄🌷 tweet media
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Jono Abshier
Jono Abshier@ExtremeGermJono·
Riddle me this @ParafilmM Do we think an undergraduate student, graduate student, post-doc or PI did this? 🙂
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Jono Abshier retweetledi
introvert
introvert@introvertsmemes·
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Sigma-Aldrich
Sigma-Aldrich@SigmaAldrich·
You see our bottle? We keep it very classy, very mindful, very demure.
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