Niyi Fadeyi

The final version of this publication has appeared in @NatureChemistry : rdcu.be/deCGD

Our pre-print describing a near-infrared light-based photocatalytic labeling technology with applications in peptide stapling and profiling of normal and patient-derived tumor tissue. chemrxiv.org/engage/chemrxi…

Happy to share that this preprint from @RovisGroup has found a home at @NatureChemistry nature.com/articles/s4155… A start of much more to come in this exciting area!

Excited to see our pre-print officially out. Thank you @NatureChemistry for publishing this work! Shout-out to @yizheng_art for the artwork including this awesome TOC graphic! nature.com/articles/s4155…

It’s been a long time coming! Our red-light photoproximity labeling method is on @NatureChemistry! We can form triplet nitrenes using low energy light, enabling its use in targeted labeling of local protein environments on live cells. nature.com/articles/s4155…

We often compare this to the goldilocks principle where the reactive intermediate half-life and rate of generation combine to play keys roles in profiling protein neighborhoods.

On the other end, using a peroxidase-based method we observed promiscuous labeling on both interacting cells leading to enrichment of 100s of proteins. While RFT and HRP both rely on phenoxy radicals, rate of generation is likely responsible for the different labeling patterns.

This highlights the importance of reactive intermediate half-life on labeling radius and how it translates in profiling different environments. Nevertheless, while both methods can profile protein communities in monoculture systems, RFT is preferred for cell-cell synapses.

PD-L1 targeted labeling in the synapse by RFT strongly enriched PD-L1, PD-1, and synaptic proteins (middle, bottom panel). While Ir PC is ideal for labeling highly localized protein environments, it performed poorly in synaptic protein enrichment (left, bottom panel)

We are often asked how our photocatalytic technologies (i.e. Iridium PC/diazirine and Flavin PC/biotin phenol) compare in mapping biological environments. In this work, we compared 3 proximity labeling technologies that rely on carbene or phenoxy radicals for labeling.

Protein communities that assemble between physically interacting cells play important signaling roles. In this preprint, we report a Flavin-dependent proteomic-platform to identify these synaptic protein environments. biorxiv.org/content/10.110…

Excited to share our work on developing a photocatalyst-based #ViraMap platform and profiling #SARSCoV2 spike protein interactions on the cell surface. Huge thanks to @OslundRob @FadNiyi @ErikHett @AndrewEmili and @drmohsan for this amazing collaboration! biorxiv.org/content/10.110…

High resolution photocatalytic mapping of SARS-CoV-2 Spike protein-host cell membrane interactions dlvr.it/SXknM5

𝗧𝗶𝘁𝗹𝗲: High resolution photocatalytic mapping of SARS-CoV-2 Spike protein-host cell membrane interactions. 𝗔𝘂𝘁𝗵𝗼𝗿𝘀: Suprama Datta, Alexander H Tavares, Tamara Reyes-Robles, Keun Ah Ryu, Nazimuddin Khan, Tyler J Bechtel, ... biorxiv.org/cgi/content/sh…

High resolution photocatalytic mapping of SARS-CoV-2 Spike protein-host cell membrane interactions biorxiv.org/cgi/content/sh… #bioRxiv

RTKs play important signaling roles in disease biology. Using clinically-advanced mAb- and bispecific-PC conjugates, we map EGFR and c-MET microenvironments in lung cancer systems to enable the development of new therapeutic approaches. pubs.acs.org/doi/10.1021/ac…

Excited to share this latest work exploring the mechanism behind our flavin-based photocatalytic proximity labeling platform. A great collaboration with the @Mat_Frenette lab and a fantastic effort by @TaylorOHope in driving this! chemrxiv.org/engage/chemrxi…

Our August issue (go.nature.com/3cSVoee) features the paper from @OslundRob, @tamarareyesr and @FadNiyi depicting immune synapses selectively and covalently tagged with biotin tyramide through PhoTag technology. go.nature.com/3zs69vP

Fantastic @nchembio cover art highlighting flavins for photocatalytic-based proximity labeling within intercellular environments that was wonderfully done by @yizheng_art. More exciting efforts coming up in this space! nature.com/nchembio/volum…

So excited to share a cover art that just came out at @nchembio.The cover image depicts immune synapses selectively and covalently tagged with biotin tyramide through PhoTag technology. Always been a pleasure to work with @OslundRob @tamarareyesr @FadNiyi nature.com/nchembio/volum…