Ferdinand Greiss

stretching chromosomes, mapping their DNA/protein organization, running cell-free gene expression directly on them, and dissecting non-eq vs eq compaction. Chromosomes can show a lot when put in cell-like compartments. let me know what you think. nature.com/articles/s4146…

Can a pandemic be stopped before it even begins? A new tool developed by Weizmann's researchers could help prepare us for the next viral threat >> bit.ly/Biochip-Eng

My latest article is out! 1000 artificial cells synchronize in 2D and collective behavior emerges! #microfluidics #artificialcells #syntheticcells #cellfreeexpression doi.org/10.1038/s41467…

I would like to share our pre-print. Autonomous biogenesis of the entire protein translation machinery excluding ribosomes biorxiv.org/content/10.110…

While writing these posts, I remembered my initial ideas probably already 3-4 years back (😅). I sketched my first scientific animation, put it aside, and forgot about it. The details changed, of course, but I hope you can still see the connection and enjoy my debut as animator.

And here is a small teaser for some (but certainly not all) of the tech development required to reconstitute chromosomes with steady-state gene expression.

Latest preprint on transplanting chromosomes into microfluidic biochips and rebooting the genome with cell-free gene expression. The deconstruction into a simple artificial system revealed fascinating DNA swelling dynamics with transcription. Enjoy! biorxiv.org/content/10.110…

I'm thrilled to share the preprint of our latest work on antibody profiling using a cell-free multiplex platform. Cell-free immuno-profiling on a genetically programmed biochip biorxiv.org/content/10.110…

Researchers at Weizmann Institute observed, for the first time, a protein being born from a single DNA molecule outside a cell. This breakthrough led them to develop the smallest-ever genetic circuit, paving the way for future nano-biotech applications >> bit.ly/DNA-circuit-Eng

Check out our new post! With German and American colleagues, #RoyBarZiv's team has identified and characterised a gene regulation mechanism that could be used in the design of synthetic cells to optimise resources and functions @WeizmannScience @FeSchiessl syntheticcell.eu/a-possible-new…

Beyond cells: Unveiling the potential of genetic circuits on single DNA molecules @NatureComms phys.org/news/2024-02-c…

And now officially available with @NatureComms at nature.com/articles/s4146…. Thanks to all X-having authors @NicolasLardon, @thenoireauxlab, and X-less authors!

Observing genetically encoded proteins emerging one by one from single DNA molecules. I am happy to share new techniques, findings about the basics of coupled transcription and translation, and genetic circuits without a cell. biorxiv.org/content/10.110…

Large scale integration of artificial cells in a microfluidic chip !! prepublished on bioRxiv doi.org/10.1101/2024.0…

@OlivierDuss That’s exciting! We recently also found gene regulation of proteins when produced locally on single DNA by the expressome (biorxiv.org/content/10.110…). Would be nice to combine these methods…

Overall, our data mechanistically highlight an example of how macromolecular machines central to gene expression physically and functionally cooperate!

Excited to share our FIRST @biorxivpreprint of the lab all done by SuperScientist Nussi Qureshi! We see how single ribosomes slow down upon colliding into RNA polymerase and that they activate RNAP via long-range physical coupling! Check it out! @embl biorxiv.org/content/10.110…

Very excited to share our latest preprint! We finally achieved the reconstitution of ribosome self-replication outside a living cell. (1/9) biorxiv.org/content/10.110…

イノシシかよ

A little sign of hope and sanity: Jews and Arabs demonstrating together: timesofisrael.com/jews-and-arabs…

Cubes, cubes cubes!📦Do you want to have TheBOX or a handy microscope?🔬How many #openUC2 cubes does the world need?🌍 Help us to find out by filling out our Demand Survey: forms.gle/y6oAH8tsxNgedp… Please retweet! 💚 #openhardware #opensource #OpenEducation

Want to use creative approaches to construct new biological systems from scratch? My lab has an open PhD position on engineering synthetic compartments in cell-free reactions. hn-lab.org/open-positions Please retweet!