Gunnar Schotta

Setdb1 silences ERV derived cryptic enhancers to ensure proper hematopoiesis. Although transcription factors, such as PU.1, can bind to H3K9me3 heterochromatin, their activity is inhibited by Setdb1. out in PNAS: doi.org/10.1073/pnas.2…

@JakobssonLab @M_Vincendeau @ch_douse Great story! Interesting that loss of DNMT1 de-represses LINE1 while "silencing mark" H3K9me3 is maintained. Much like we found in mouse endoderm IAPEz elements are derepressed in Dnmt1 ko with maintained H3K9me3 rdcu.be/cVRqS

New transposon preprint! We are extremely excited about this collaborative work led by the @ch_douse lab. A mechanistic dissection of the interaction between DNA-methylation and HUSH in the control of LINE-1s in human somatic cells. Such a cool system…🧬 biorxiv.org/content/10.110…

@ControllingLMNT @LickertHeiko @NatureComms I think there will be heterogeneity. For example, an IAP element in the Mnd1 locus seems specifically regulated in neuronal cells (PMID 22991445,27798843), but not in XEN or DE cells. Maybe due to differences in TF networks. Will need to investigate better...

@GSchotta @LickertHeiko @NatureComms Awesome! Any idea if there are "jackpot" IAP that preferentially are sensitive to loss of Dnmt1 & contribute to this pattern in XEN/dEnd cells, or do you think there is homogeneity in response across most instances (copies) of IAP?

H3K9me3 is not sufficient for silencing endogenous retroviruses if DNA methylation is perturbed. In Dnmt1 ko endoderm cells, IAP elements fully maintain H3K9me3, while trancriptionally active. great collaboration with @LickertHeiko out in @NatureComms rdcu.be/cVRqS

@ControllingLMNT @LickertHeiko @NatureComms Very good question. Unfortunately read mapping on individual IAPs is challenging due to strong homology/identity between elements. Long read sequencing will help to clarify this better.

@TheBraunLab @LickertHeiko @NatureComms Thank you! And congrats to your nice Lem2-exosome story!

@GSchotta @LickertHeiko @NatureComms Congratulations !

DNA sequence-dependent formation of heterochromatin nanodomains [Graeme Thorn, Christopher Clarkson, Anne Rademacher, Hulkar Mamayusupova, Gunnar Schotta, Karsten Rippe & Vladimir Teif] nature.com/articles/s4146… ChromHL software here: github.com/TeifLab/ChromHL Cartoon as a bonus!

Here is our Snapshot of Human Endogenous Retroviruses (HERVs) that I wrote together with @JakobssonLab for Cell (@CellCellPress). Enjoy reading here: authors.elsevier.com/a/1eS8aL7PXf0k2 - @HelmholtzMunich @Lund_Stem

@simonelsasser Thank you @simonelsasser. Much appreciated :-)

G-quadruplexes are cell type-specific and associated with high transcription. Great collaboration with @RichterLabUnipd @SaraNRichter_

Morc3 silences endogenous retroviruses by enabling Daxx-mediated H3.3 incorporation. Morc3 GHKL ATPase domain dimerizes for Daxx interaction. An efficient ATPase cycle is critical for H3.3 incorporation. biorxiv.org/cgi/content/sh…

HIV infection activates ERVs to regulate antiviral gene expression. Great collaboration headed by Daniel Sauter! academic.oup.com/nar/advance-ar…

G4 quadruplex structures are cell type specific and associate with highly expressed genes. New preprint of a collaborative project led by @SaraNRichter_ biorxiv.org/cgi/content/sh…

Ever wonder why children react differently to pathogens? Join our lab and help us find out! uni-muenchen.de/aktuelles/stel…

The Kidney Contains Ontogenetically Distinct Dendritic Cell and Macrophage Subtypes throughout Development That Differ in Their Inflammatory Properties @buschraml @gschotta @FCernilogar bit.ly/30jo4CY