Harvard Cryo-EM Center for Structural Biology

Check our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3) 🔗 biorxiv.org/content/10.648…

Excited to share our latest work led by our postdoc Dan Zhao! We uncovered how the bifunctional complex of Mnl1 mannosidase and protein disulfide isomerase (Pdi1) initiates ERAD. A long-sought ERAD reductase is finally discovered! #Sec11" target="_blank" rel="nofollow noopener">nature.com/articles/s4159… Huge congratulations!

Excited about mechanistic biology and processes in the cell's nucleus? Join our lab as a post-doctoral researcher! We have a state-of-the-art cryo-EM facility at HMS, a brand-new lab space, and a vibrant community at HMS! DM/email me or check farnunglab.com

Our story on the mechanism of co-transcriptional histone mark deposition is now officially out: science.org/doi/10.1126/sc…

Absolutely thrilled to share paper on Nipah polymerase in close collaboration with Rachel Fearns at Boston University @BU_Tweets. 😃We previously posted on @biorxivpreprint but this is the print form @CellCellPress. 🦇 @HarvardMicro @gatesfoundation cell.com/cell/fulltext/…

Excited to share our @biorxivpreprint led by @xiaoyi_fann and @wanyuviridae exploring how binding of western equine encephalitis virus (WEEV) to its cellular receptors evolved over the past century 🤩 biorxiv.org/content/10.110… @HarvardMicro

1/n) Best way to start 2025! Thrilled to share our Nature paper about the structures of axonemal components from sperm flagella and from epithelial cilia of the oviduct and brain ventricles. Great collaboration with @TzviyaZBM and @alanbrownhms nature.com/articles/s4158…

Ever wondered how transcription choreographs histone modifications? Our work reveals the basis of co-transcriptional H3K36me3 by SETD2. We visualize how a histone writer coordinates with the transcription machinery! This is the magnus opus of @MarkertJon tinyurl.com/setd2

A nice piece on one our @CellCellPress papers published earlier this year about mechanisms of herpes simplex virus polymerase drug resistance related to conformational dynamics. 🕺 drugdiscoverynews.com/protein-confor…

Excited to share our most recent paper at @CellCellPress in which we used #CryoEM to study conformational dynamics as a determinant of drug selectivity and resistance for the herpes simplex virus DNA polymerase. cell.com/cell/fulltext/…

We are excited to share our @NatureComms paper by postdoc Pan @an32169364 and @VirologyHarvard grad student Wanyu @wanyuviridae on the structural binding mode of eastern equine encephalitis virus (EEEV) and Semliki forest virus (SFV) with receptor VLDLR rb.gy/2ryjcu

Congrats everybody, that took a while from the preprint 😅💪 nature.com/articles/s4158…

A new paper from Benjamin Ebert, Nathanael Gray, @eric_fischer, @fischerlab1, @LiYender, Michelle Ma, and @M_MurtazaHassan reports a trans-labeling mechanism “template-assisted covalent modification”, which enables proximity-driven pharmacology rdcu.be/dPzUW

STK19 is a bona fide transcription-coupled repair factor. Check out the preprint with @FrogWalterLab ➡️bit.ly/4fgl2Fq We achieved a major technical milestone: 1.9 Å resolution structure of an RNA polymerase II transcription complex. Txn goes sub-2 Å!

Excited to share our story on polymerization in ZBTB transcription factors, which was published in the July 11 issue of @MolecularCell! cell.com/molecular-cell… 1/n

Excited to share @biorxivpreprint in collaboration with Rachel Fearns at BU in which we determined the cryo-EM structure of the Nipah virus polymerase complex! The complex assembles with a tobacco pipe-like shape with a flexible stem (do you see it?)😃😅biorxiv.org/content/10.110…

Antibodies expand the scope of angiotensin receptor pharmacology | Nat Chem Biol doi.org/10.1038/s41589…

The 20S proteasome plays a key protease role in many regulatory processes. Yet, how its ordered biogenesis and temporally timed catalytic site activation works has remained elusive. In our latest work, we provide evidence for how that occurs! @NatureSMB rdcu.be/dElr4

The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2 dlvr.it/T54tGQ

Thanks @Jasonmmast for covering our new work with @fischerlab1 on DCAF5 in @statnews today.