Marilina de Sautu

Check our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3) 🔗 biorxiv.org/content/10.648…

In just the last 20 years, beef production destroyed global forests the size of California, mostly in the tropics Through tree loss alone (not including methane or feed crop production), this caused 3x the annual CO2 emissions of the US It's the cow, not the how

Dissociated VP7 monomers form a single, large, cooperative pore, seen both in cells and in liposomes. An amphipathic VP7 helix is sufficient for perforation, enabling rapid DLP release and immediate viral transcription in the cytosol (3/3).

We show that entry is a two-step membrane insertion process. VP4 spikes switch from upright (attachment) to reversed (insertion of foot domain). This triggers Ca²⁺ leakage from vesicle, Ca2+ signaling and ultimately VP7 dissociation (2/3)

Check our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3) 🔗 biorxiv.org/content/10.648…

Mechanism of membrane perforation in rotavirus cell entry de Sautu, M., Leistner, C., Kirchhausen, T., Jenni, S., Harrison, S. C. biorxiv.org/content/10.648…

MOSAIC in vivo two-photon imaging in the mouse cortex with adaptive optics (AO) to correct sample-induced aberrations: neural activity; erythrocyte flow in capillaries; large field of view of dendrites and vasculature; and dendritic structure with and w/o AO vs depth.

Donating what means a little bit for you, you can help feed so many. Please join Fraternity without Borders. Help the orphans in Congo. 🙏🏽❤️‍🩹 youtu.be/c9SZ1hROxwg?si…

Why do we claim to cherish some animals while seeing others as food? Are dogs and pigs really that different? Let’s dig into the science, psychology, and ethics behind this divide. 🐕🐖 #speciesism #doglover #dogs #pigs #ethics  veganfta.com/2025/04/02/are…

Merry Christmas and our best wishes for a peaceful 2025. Say goodbye to the noise with CryoSamba, our self-supervised deep denoising algorithm for CryoET now published in JSB.

Unexpected presence of intrinsically perforated endolysosomes in neurons suggests involvement in the genesis of toxic α-syn aggregates induced by internalized pre-formed fibrils (PFFs). New from Sanyal et al. @KirchhausenLab @harvardmed: hubs.la/Q030wbQC0 #Neuroscience

Brookline pets 🫶🏽

⚠️ BREAKING: California reports two new human cases of H5N1 bird flu, bringing the statewide total to 29. 58 cases have been reported nationwide this year.

🤯 beautiful!

The academic job market in the good ol' days vs the academic job market today.

Three months after Javier Milei took office as the new president of Argentina, scientists there say that their profession is in crisis go.nature.com/3Tpm8Wx

68 Nobel laureates acknowledging the outstanding contributions of Argentinean Science and Technology to the world. Argentinian science is in real danger. Please help us stop this nonsense!

👉68 prestigiosos científicos y científicas ganadores del @NobelPrize firmaron una carta en apoyo al sistema científico argentino y contra el feroz ajuste al que lo está sometiendo el gobierno nacional. nexciencia.exactas.uba.ar/carta-68-premi…

Intrinsically perforated endo-lysosomes in neurons are crucial for alpha-syn aggregation. Check out our latest preprint. Another great collaboration with @NHatzakis lab. biorxiv.org/content/10.110… @harvardmed @BostonChildrens @HarvardCellBio

In our preprint #DeepSPT we show #Rotavirus's escape to the cytosol can be detected solely by change in diffusion. For more examples of capturing biology by motion: biorxiv.org/content/10.110…. @NHatzakis @CHEMUCPH @KirchhausenLab @harvardmed @ISBUC1 #deeplearning #FluorescenceFriday

How much can be extracted from diffusion of single molecules? Here we show diffusion can reveal viral escape to the cytosol, the cellular location of clathrin coated pits, and can encode biomolecular identity. Great collab @NHatzakis @CHEMUCPH and @KirchhausenLab at @harvardmed