ION_BRU

ION BRU in Glasgow is next week! There's still time to register, check out the full agenda on our Eventbrite: eventbrite.co.uk/e/ion-bru-meet…

Help! We want to run our flongles with rt base calling in the lab @RBGE, but I am bewildered by computer specs so don't know what to buy, except it has to be better than our current laptop. Exactly what machines, upgrades etc are nanopore folks using/recommending - @ION_BRU ..?

Our next ION_BRU meeting will be on 26th October, and for the first time will be held in Glasgow! Please drop by the eventbrite page to register and drop us an email if you want to present at the meeting. eventbrite.co.uk/e/ion-bru-meet…

You can also let us know you want to speak by filling out this form forms.office.com/e/uGANSbWrVm

I am happy to share that our paper introducing isONform, a tool for de novo transcriptome recounstruction from Oxford Nanopore data, is now available in Bioinformatics doi.org/10.1093/bioinf… and will be presented by me at #ISMB23 in Lyon.

@markhughesis @ION_BRU See here for cost-effective and fast protocols: m.youtube.com/channel/UC1Wow…

@markhughesis @ION_BRU Hi Mark, sounds like a cool project. The following may be a helpful starting point - please let me know if you have any questions nature.com/articles/s4159…

We are the @nanopore User Group Philippines! Established 27 June 2023 Los Baños, Laguna, Philippines

Learn how to use a workflow manager, 22 - 24 May. Running your analysis with snakemake allows you to re-run analysis, include more samples, or alter parameters with very little effort. This not only saves you valuable time but is easier to use! genomics.ed.ac.uk/services/bioin…

Haha sorry @_shannon_laura, only just noticed autocorrect renamed your Typanosoma!

Next up is @_shannon_laura with the use of ultra long reads to assemble genomes for Trypanosoma Bruce, which has some super repetitive section where important information (e.g. virulence genes) hide

It was indeed a day well spent with great talks on nanopore. Thanks @ION_BRU for this well organized event #nanoporesequencing #newuser

Thanks to the @ION_BRU crew for a great day chatting all things @nanopore. Amazing variety of subjects, talks and representation #longread #nanopore

The @rabidbites @BatsGoViral contingent from Glasgow and honorary guests from the Philippines @RITMPH at @ION_BRU today! #nanoporeMAD

Fantastic talk from @malcolmacaulay! What an amazing contribution from students, bringing #DNAsequencing to the classroom 🧬

(See the blurriness of the bulk plot on the right, versus the single molecule plot on the left)

...important information in high heterogeneity reads was being lost in the bulk reads!

Our last talk of the data is from @_Lyndsay_94, who is using nanopore to look at methylation pattern variation across single DNA molecules