James Oakley

Really nice work showcasing how tuning chemical reactivity can improve spatial resolution for proximity labeling. Super cool. Directed Evolution of APOX for Proximity Labeling Using Phenols with High Redox Potentials | ChemRxiv - go.shr.lc/43oDp7z

We're entering into a golden age in proximity labeling, and photocatalysis is a major new driver for uncovering biological mechanisms. Check out our review (@BenitoBuksh @HuthSean @DanielleMorganC ) of the latest developments out now in @CellChemBiol!

Our new proximity labeling technology is now published!! This new subgroup is doing some amazing work - more to come! nature.com/articles/s4155…

New year, new proximity labeling protocol! We can use red light to access trifluoromethyl carbenes from diazos for proximity labeling. Congrats to @crabaneros and @stavkariofillis for a great paper in collaboration with Merck! pubs.acs.org/doi/10.1021/ja…

In a major milestone for science, for medicine, and for patients, the world’s first CRISPR drug has been approved in the UK. “A new treatment for sickle-cell disease and transfusion-dependent β-thalassemia has been authorised by the Medicines and Healthcare products Regulatory Agency (MHRA) for patients aged 12 and over after a rigorous assessment of its safety, quality and effectiveness.” Congratulations to the many scientists, doctors, patients, and industry leaders who made this development possible.🍻 gov.uk/government/new…

The Geri Lab is recruiting postdoctoral scholars to develop photocatalytic proximity labeling and proteomics technologies. Chemical biologists and chemists who want to learn chemical biology are welcome! gerilab.weill.cornell.edu/team The view from our recently expanded NYC lab space:

Very cool work mapping binding sites with amino acid precision! And it’s extra rewarding when I see people I know using FragPipe 🤗

@HuthSean @CiaranSeath @Merck and shoutout to fellow @CSUSM grad @GeiszlerDaniel for the awesome proteomic software we used for this work 😎

Excited to share our latest work on small molecule binding site ID. We demonstrate this application across 7 different protein-ligand pairs and in live cells. Really fun team effort with @HuthSean @CiaranSeath @Merck and the rest of the team pubs.acs.org/doi/10.1021/ja…

What if small molecules could allow you to alter protein localization at will? What if they could allow you to turn on specific transcription factors? Our new manuscript reveals bifunctional molecules to do just that. 🧵

TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase period (minutes to hours) and then 1-minute APEX tagging in the destination compartment. Proteins tagged by both enzymes are enriched with streptavidin and anti-fluorescein antibody, and identified by mass spec. We explore 4 intracellular and intercellular applications of TransitID: - discovery of locally translated mitochondrial proteins (translated at OMM, then imported into mito matrix) - mapping proteins whose cytoplasm-to-nucleus shuttling is dampened by stress - discovery of proteins that transit between nucleolus & stress granules during stress & stress recovery - analysis of proteins that traffic intercellularly bw tumor cells & macrophages Work of amazingly talented co-first authors @WeiQinChemBio and @CheahJoleen, and collaborators Steve Carr @namude & Paul Taylor authors.elsevier.com/a/1hKLcL7PXmcqA

@robertiafe @CSUSM @calstate so great to see this! congrats

So excited to announce that Sebastian Aguero and Valeria Castellanos Rodriguez were awarded the Barry Goldwater Scholarship! They are the ⭐️first-ever⭐️ Goldwater Scholars at @CSUSM! This prestigious award recognizes leadership potential in research science. @calstate #CSTEM

Check out this preprint on cubanes. Using a convenient approach to cyclobutadiene and copper photoredox catalysis, we are able to access bioisosteres of ortho-, meta-, and para-substituted benzenes for Med. Chem.   chemrxiv.org/engage/chemrxi…

lnkd.in/gPcXteFw lnkd.in/gbiRUDet

Great collab between @PlossLab @PrincetonMolBio and @MacMillan_Lab @PrincetonChem, ID’ing 8 novel receptors for #COVID19 on the human cell, including 4 important for viral entry. Congrats to all. Story, link to @J_A_C_S paper: bit.ly/3eRiTW2

Using super-resolution microscopy, we provide some insight into the spatial selectivity (labeling radius) of proximity labeling technologies. We also developed some new ones along the way. pnas.org/doi/10.1073/pn…

Plus another @Merck collaboration with @MacMillan_Lab showcases how an additive screen can substantially broaden the scope of nickel/photoredox catalysis science.org/doi/10.1126/sc…

Excited to share this one. Great team effort.

We're hiring! We have several postdoc positions open for chemical biology research and proximity labeling in the MacMillan Lab. Experience in cell biology, biochemistry, molecular genetics, immunology, or related fields desired. DM us with your info and a CV/cover letter!

We are actively recruiting a postdoc interested in working on developing new chemical biology tools for protein interaction discovery. If interested, please email me at jag4016@med.cornell.edu! gerilab.weill.cornell.edu/team