Johannes B Müller-Reif

We compared 3 MS and 1 aptamer proteomics workflows on elite athlete plasma. Platform differences aren't just technical—they reveal distinct biology of exercise adaptation and metabolic health. New preprint just in time for Winter Olympics 🏆 🥇 biorxiv.org/content/10.648…

Discovery proteomics → clinical diagnostics? ADAPT-MS makes it possible by adapting to each sample's protein coverage on-the-fly. No imputation, no fixed panels, multiple diagnostic questions from one measurement. Out now in @NatureComms. nature.com/articles/s4146…

Latest publication in @molcellprot “A simplified perchloric acid workflow with neutralization (PCA N) for democratizing deep plasma proteomics at population scale” by @_VAlbrecht et al. mcponline.org/article/S1535-… @ASBMB @labs_mann @JoBMller

New approach tackles studying rare pediatric diseases! We developed an ontology-guided clustering framework using SNOMED CT to analyze proteomes from 1,140 children across 394 conditions - enabling meaningful insights even with tiny patient numbers! embopress.org/doi/full/10.10…

To bead or not to bead? We evaluate five plasma proteomics workflows, finding bead-based methods enhance detection but show high susceptibility to cellular contamination bias. Strategies to optimize biomarker discovery in plasma proteomics. doi.org/10.1101/2025.0…

Check out ADAPT-MS: our flexible, scalable diagnostic framework that transforms discovery plasma proteomics directly into clinical decisions. Dynamic retraining enables single-sample diagnostics for any task - no imputation or fixed panels needed. doi.org/10.1101/2025.0…

Our PCA-N method with neutralization step enables direct enzymatic digestion, doubles proteome depth from 5µL neat plasma, and scales to 10K+ sample preps/day. Democratizing plasma proteomics with validation across 50K measurements. On @biorxivpreprint! biorxiv.org/content/10.110…

🔓 #OpenAccess + Most Read in the past 30 days: "The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends" go.acs.org/bEe

The 68th Benzon Symposium report is now online in @molcellprot ! Fantastic conference that brought together top global experts in Copenhagen to translate proteomics to the clinics - from technological advances to regulatory frameworks! @labs_mann bit.ly/3AsuN3X

Are you interested to learn more about the circulating blood proteome? If so, here's a review that covers new and emerging technologies, advances, challenges, proteoforms, vesicles, protein networks, antibodies, and microsamples. @hupo_org pubs.acs.org/doi/10.1021/ac…

The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends #JProteomeRes pubs.acs.org/doi/10.1021/ac…

If you are at #hupo2024 in Dresden, do catch up with our lab-mates to know more about some of our exciting research in @labs_mann 👇

Fantastic four day symposium in beautiful and sunny Copenhagen: "MS Proteomics + AI in Personalized Medicine". Kudos to the Benzon Foundation, our fantastic speakers, and all participants. The ideas and collaborations sparked here will move clinical proteomics forward!

Happy to announce that our new lab in Berlin @dhzcharite is recruiting! We are offering PhD and postdoc positions. Join our exciting journey to discover and to translate novel mechanism in thromboinflammation - within an fantastic team -apply: karriere.charite.de/en/job-vacanci…

Incredibly proud of Matthias Mann for winning this year's Dr. H.P. Heineken Prize for Biochemistry and Biophysics together with Ruedi Aebersold! Their pioneering work has transformed protein research and is reshaping the future of biomedicine! #proteomics heinekenprizes.org/2024/06/11/rue…

Missed the Thermo User meeting at on Sunday on the new targeted MS? Our preprint on the Stellar MS shows how to use it in combination with the Orbitrap Astral and Evosep One to design 15N labeled protein based assays. Check out Maria Wahle’s poster ThP733 biorxiv.org/content/10.110…

A novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N labeled proteins dlvr.it/T7lZDf

(BioRxiv All) A novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N labeled proteins dlvr.it/T7lgnm (RSS) #BioRxiv #MassSpecRSS

A novel hybrid high speed mass spectrometer allows rapid translation from biomarker candidates to targeted clinical tests using 15N ... biorxiv.org/cgi/content/sh… #biorxiv_biochem

1/7 @labs_mann and Georg Wallmann @GeorgWa are proud to announce alphaDIA, our open source, next generation DIA search engine. Check out the preprint @biorxivpreprint biorxiv.org/content/10.110…