Special thanks also to @HDKaul for excellent collaboration, @vancityphd @jonesr18 and all previous and current @StemCellEng team members for their hard work and to @PZandstra for continuous support and superb guidance
English
Laura Prochazka
379 posts
Laura Prochazka
@LauraProchazka
Synthetic Biology, Stem Cell Engineering, Cell-based Therapies. Scientist at Notch Tx.
Toronto, Ontario Katılım Ekim 2016
974 Takip Edilen445 Takipçiler
Extremely thankful to @YaleMichaels whose brilliant mind and previous inventions were key in realizing this work.
English
Excited to see this out!
Peter Zandstra@PZandstra
Synthetic gene circuits for cell state detection and protein tuning in human pluripotent stem cells Excited about this work by @LauraProchazka and team at @StemCellBioEng. Great collab w/ Y Benenson @ETH_en #synbio #stemcells @SBME_UBC @ubcmsl embopress.org/doi/10.15252/m…
English
Laura Prochazka retweetledi
What do I, @nzmyachie and Dr. Josef Penninger (@PenningerLab) have in common?
We are each looking for postdocs for a joint project combining #synbio & #organoids! Spread the word 📢 & apply!
Shakiba: tinyurl.com/shakibaPDF
Yachie : tinyurl.com/yachiePDF
Penninger: email 📧
English
Laura Prochazka retweetledi
Came across some great articles for those interested in using #synbio approaches to control stem cell fate🧵.
English
The Aregger Lab is officially open! 🥳 PostDoc positions for experimental and computational biologists are available, get in touch if you're interested to join our team.
ccr.cancer.gov/staff-director…
English
@Yassi_ag @CellStemCell @YYZscientist @mcnostro @McEwenInstitute @physiologyuoft @bme_uoft @LMP_UofT @frankiepoonhc @FaridaSarangi Congrats Yassi!!
Français
Excited to share our paper on #vascularization of #stemcell-derived pancreatic progenitors & human islets in diabetic mice @cellstemcell cell.com/cell-stem-cell…
@YYZscientist @mcnostro @McEwenInstitute @physiologyuoft @bme_uoft @LMP_UofT @frankiepoonhc @FaridaSarangi we show:
Cell Stem Cell@CellStemCell
New Article online now CellStemCell: Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models zpr.io/uEE9AZ5bFpDD
English
Laura Prochazka retweetledi
Paper finally published @NatureComms!
Press release from Notch Therapeutics on the development and implications of our platform technology below👇🏾
Tweetorial on paper (+ acknowledgement of my wonderful team) coming soon🙏🏾🧵
@Sunnybrook @UofTMedicine
notchtx.com/press_release/…
English
@ATrotmanGrant @PZandstra @YaleMichaels @johnmedgar Wow! Congrats Ash!! looking forward to many more conversations :)
English
@PZandstra Thank you @PZandstra! It's taken a while but I'm glad to finally see it out. I very much appreciate the support from you and all the wonderful conversations I've had over the years with your lab members (@LauraProchazka @YaleMichaels @Johnmedgar). Now time to graduate😅...
English
Huge congratulations @ATrotmanGrant #tourdeforce
Ashton C Trotman-Grant@ATrotmanGrant
Paper finally published @NatureComms! Press release from Notch Therapeutics on the development and implications of our platform technology below👇🏾 Tweetorial on paper (+ acknowledgement of my wonderful team) coming soon🙏🏾🧵 @Sunnybrook @UofTMedicine notchtx.com/press_release/…
English
Laura Prochazka retweetledi
@GallowayLabMIT @JoshTycko Good to know! Always puzzled me why large constructs perform so much worse. Wrongly assumed it must be because of reduced delivery efficiency. Looking forward to learn more about this work!
English
@JoshTycko Thus, I would expect that the longer blast gene effectively silences the gRNA via a wake of positive supercoiling that inhibits transcription of the gRNA...want to hear more? Come to mSBW!
English
CRISPR vector design Q:
Hill et al. says this design with convergent Pol2 and Pol3 transcripts has 3x reduced editing efficiency; they hypothesize Blast transcription inhibits sgRNA expression (Nat Meth 2018).
Anyone know of other data from convergent designs? Thx! 🧬⭕️✂️
English
@jonesr18 @Leili_Rohani Tricky but powerful sums it up nicely! ;)
English
@Leili_Rohani Are the cells all coming from one source, eg PSC-derived, or do the cells you want to stop proliferating get added in separately? For the former you could consider something like a cell classifier (@LauraProchazka). They can be tricky but powerful for things like this.
English
Engineering cells is hard; how can we ensure engineered functions perform well under varying extracellular and intracellular contexts? @nika_shakiba and I review these challenges, solutions from control theory, and applications for reg med/cell therapies! doi.org/10.1016/j.cels…
English
Nice work! Congrats @jonesr18 and @nika_shakiba!
Ross D. Jones, PhD@jonesr18
Engineering cells is hard; how can we ensure engineered functions perform well under varying extracellular and intracellular contexts? @nika_shakiba and I review these challenges, solutions from control theory, and applications for reg med/cell therapies! doi.org/10.1016/j.cels…
English
Laura Prochazka retweetledi
'Science Startups, Who's Hiring?' (June)
Last month I made a thread to help scientists / others find cool roles at startups. Seemed helpful, so doing again!
Science startups -- reply with 1 tweet on your mission + open roles.
Everyone -- please RT so more people see!
(🧵 1/2)
English
Our new pre-print is out! We show that GP2 enriched pancreatic progenitor population give rise to functional beta cells in vivo while eliminating teratoma formation.
Nostro Lab@MCNostroLab
Excited to share our new pre-print on the in vivo developmental potential of GP2-enriched pancreatic progenitors biorxiv.org/content/10.110… @Yassi_ag @FaridaSarangi @frankiepoonhc @blessingnkennor @EmMcgooo @YYZscientist @mcnostro
English
Laura Prochazka retweetledi
Today in @NatureComms we speak on how co-transfection experiments are affected by limited intracellukar resources! hope it helps!
thanks @ceronifranci @IITalk
Francesca Ceroni@ceronifranci
Our take on how resource competition impacts co-transfection experiments in mammalian cells is out now: nature.com/articles/s4146…. Thanks @NatureComms and @rosscloney, we are very happy of our collaboration with Dr Karen Polizzi and @VeliaSiciliano
Naples, Campania 🇮🇹 English
Laura Prochazka retweetledi
We are recruiting at multiple levels! Interested in developing CRISPR and AAV technologies while working with a great team in Switzerland? We are seeking applications for a Lab Manager, Postdoc, and Research Technician. More info at: bsse.ethz.ch/platt/join-us.…
English
@GeorgeMonbiot @GretaThunberg Watched it yesterday. And cried.
English
1. I watched #Seaspiracy on Netflix last night. It’s a brilliant expose of the greatest threat to marine life: fishing.
This thread explores why we have so seldom heard the the truth about what’s happening to our oceans.
#TellTheTruth
@GretaThunberg
netflix.com/gb/title/81014…
English
Laura Prochazka retweetledi
Looking forward to our discussion today!
Maria Abou Chakra@MariaAbouChakra
Join our next Modelling Cell Development & Regeneration Discussion group. Pls Register here: utoronto.zoom.us/meeting/regist… Supported by @MbD_UofT @SMBdevBio @garybader1 Pls RT @the_Node @___SDB___ @Dev_Bio_Journal @SMB_MathBiology @StemCellNetwork @ISSCR @CCRM_ca @OIRMnews
English