Paul Markus Müller

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Paul Markus Müller

Paul Markus Müller

@P_M_Mueller

Postdoc in @ewers_helge lab, @FU_Berlin, grappling with stubborn #Septins in the lab and with stubborn kids outside the lab

Berlin, Deutschland Katılım Haziran 2018
747 Takip Edilen412 Takipçiler
Raluca Groza
Raluca Groza@RaluucaG·
Thrilled to start my postdoc in the Kleele lab @tatjana_kleele at @ETH in beautiful Zürich. I will be looking into how local protein translation regulates mitochondrial dynamics in neurons 🎉🇨🇭
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André Nadler
André Nadler@nadlerlab·
Here’s the big one: In collaboration with @Honigmann_Lab, @LabShevchenko, Björn Drobot and Martin Hof we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. biorxiv.org/content/10.110…
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Urban Cycling Institute 🚲 
Urban Cycling Institute 🚲 @fietsprofessor·
'Cycling is fun and games, but we simply need cars to get our groceries!!1!' 🇳🇱 Dutch guy: "Hold my @Grolsch🍺. Literally"
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Raluca Groza
Raluca Groza@RaluucaG·
Glad to see my PhD work from the @ewers_helge lab out now in @NatureComms. Check it out if you want to know more about the biophysics behind lipid-mediated endocytosis.
Ewerslab@ewers_helge

@raluucaG now asked: What are the requirements to form these tight-fitting early endocytic carriers? She made a synthetic receptor-ligand system. Genetically encoded nanoparticles (GEMs), 37 nm spheres studded with 180 GFPs and lipid-anchored anti-GFP nanobodies as receptors.

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Ewerslab
Ewerslab@ewers_helge·
Paper out: Gene encoded nanoparticles (GEMs) with 180 GFPs are cool tools to track in cells. GEMs reveal biophysical basis of infection, too. Using GPI-anchored nanobody receptors over 6 logs of affinity shows that adhesion energy alone explains uptake! rdcu.be/dFddO
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nadjahuempf.bsky.social (she/her)
nadjahuempf.bsky.social (she/her)@MforMicroscaya·
Finally it is out 😁. Our review on #expansion microscopy summarizes the key steps of sample prep and gives an overview of the different methods. Perfect for novices to the field 🧑🏽‍🔬
Ewerslab@ewers_helge

ExM-review online! @MforMicroscaya @RiaThielhorn and I describe how ExM works, how to do experiments and control them properly. Plus list of everything you need to know and where to find it! Thx to @AliHShaib Silvio Rizzoli & @HofkensLab for sharing data. journals.biologists.com/jcs/article/13…

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Paul Markus Müller
Paul Markus Müller@P_M_Mueller·
this is my last desperate attempt to reach the people in the lab, if it doesn't work i'll give up and lose my faith in humanity for good
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Paul Markus Müller
Paul Markus Müller@P_M_Mueller·
@ewers_helge Background light is probably from the sparkling eyes of a postdoc who‘s happy to be back to TIRF microscopy after quite a while 🥹
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Ewerslab
Ewerslab@ewers_helge·
@P_M_Mueller Either more that one laser is on or it is sunset in the microscopy lab
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Paul Markus Müller
Paul Markus Müller@P_M_Mueller·
Total internal reflection visualisation is never getting old
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Raluca Groza
Raluca Groza@RaluucaG·
just when I thought getting a PhD was hard… ohh boyy 😅
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Ewerslab
Ewerslab@ewers_helge·
You need a proteins, but a few only and delivered in a non-invasive manner? For single molecule imaging maybe? Or to reconstitute functioning a knockout? @purbakashyap has the solution! So excited to see it out. Constructs soon on @Addgene nature.com/articles/s4159…
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Keşfet

@RaluucaG @tatjana_kleele @ETH @Fran_Bottanelli @petiaadarska @sPersson96 @CvijovicLab @frank_pado