John Ridenour

APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2 is a liability. We now report LaccID, a multicopper oxidase evolved from an ancestral fungal laccase that oxidizes aromatic substrates using non-toxic O2 instead of H2O2. We use LaccID for EM imaging of cultured cells and fly brains, and for proteomic mapping of the changing surface composition of T cells that engage with tumor cells via antigen-specific T cell receptors.

I have written a concise review on chromatin transcription as part of the @JMolBiol special issue on transcription elongation. Check it out! authors.elsevier.com/sd/article/S00…

🚨Preprint alert v.2🚨🤩 I am super excited to share the preprint of another of my PhD chapters in #Zymoseptoria on BioRxiv: "Interspecies hybridization as a route of accessory chromosome origin in fungal species infecting wild grasses"🧵👇(1/8) biorxiv.org/content/10.110…

Coming up on October 9th - talks from three amazing early career researchers: @n_sanchirmaa, @Heankel_, and @AbidKhanEpigen! Register now: us06web.zoom.us/webinar/regist…

The first paper from our lab is now out in peer-reviewed form @LSAjournal. Huge congratulations to the first author Mary Bergwell, @STEMinistSkater, now in grad school at UPenn, and second author @JY_Park102! I am very proud of their hard work. They even let me do a few expts!

New Preprint We discovered that RNAi loss is not uncommon in the global population of Cryptococcus. Our findings suggest that many of them could become hypermutators following introduction of a transposon burden, including not only Cnl1 but also large KDZ transposons🧬.

2024 Langston Scholar J’Taelii Heath and OMRF scientist and student liaison Dr. Valerie Lewis were featured on @koconews to highlight the program's impact on LU students. 📺Watch now: koco.com/article/oklaho…

The Lewis Lab at UGA is searching for a research technician to support two NIH-funded projects. You can learn about our work at lewislab.uga.edu. Apply here: ugajobsearch.com/postings/391610

New funding mechanism from NSF for institutions to support GRFP honorable mention awardees in these states/territories: AK AR DE GU HI ID IA KS KY LA ME MS MT NE NV NH NM ND OK PR RI SC SD VI VT WV WY Thanks @hormiga for the heads up 🧪🌿🐸$$$ new.nsf.gov/funding/opport…

How does RNA flow through the cell? From their synthesis, chromatin release, nuclear export, ribosome engagement, and eventual degradation, we quantified the lives of human transcripts genome-wide. (1/7) See @BrendanSmalec and @robertietswaart ‘s preprint biorxiv.org/content/10.110…

YeastMine is closing July 15 due to ongoing funding cuts. For more info: community.alliancegenome.org/t/yeastmine-sh… #yeast

Heard today: "We also labelled microtubules because they look cool" @egan_martin #FungalGRC24

Happy first day for OMRF's Summer Scholars! We are so excited you are here! Sir Alexander Fleming Scholars, Langston University Biomedical Research Scholars and John H. Saxon Service Academy Summer Research Program students will work with OMRF scientists on research projects.

How do chromatin modifications quantitatively impact gene expression? How do sequence variants or combinatorial marks influence relationships? We developed a modular #EpigenomeEditing toolkit to systematically test these Q, published today 🧵… nature.com/articles/s4158…

Come and join us! Really nice environment with great people and science.

It was great to catch up with past folks from @ArkansasEPP at #Fungal24

Team @novonesis at #Fungal24

Next up in the plenary, Zachary Lewis @UgaChromatinLab on his excellent epigenetic work in Neurospora #Fungal24

Made it to Asilomar for the #fungal24 Fungal Genetics Conference representing @novonesis @NovozymesNA

Publication alert ! Paper from helmholtz-munich.de/en/ife in @Nature How complex epigenetic modification signatures regulate genome function. With interactive online resource marcs.helmholtz-munich.de Big data ! @epigeneticsHMGU @HelmholtzMunich nature.com/articles/s4158…