Slawomir Michniewski

with @RyanCookAMR , we have updated complete genomes we can find Genbank and made them available with stats etc millardlab.org/phage-genomes-…

As ever, this month's files all on Github. Great to see the updated taxonomy available too 👀 #phages

5 undergrads with zero phage experience. 10 weeks later 30+ isolates , 27 phage genomes + TEM images for all, 7 phage with one steps experiments... not to shabby. under expert guidance of @phagegeek @SMichniewski

Genome Sequence of SN1, a Bacteriophage That Infects Sphaerotilus natans and Pseudomonas aeruginosa Interesting! 2 bacteria that belong to 2 different classes. Gammaproteobacteria and Betaproteobacteria. I wonder what receptor they share. @smoineau journals.asm.org/doi/10.1128/mr…

Could you please with any graduating BSc or MSc students that may be interested in working with lysogenic @phages and their role in adaptation and evolution of chronic bacterial lung infections.

Latest from my lab looking at a so called antrax “cross over”strain (B. cereus G9241). Great work by a superb team, @warwickmed Tom Brooker, Grace Taylor Joyce, Alexia Hapeshi and Shathviga Manoharan. All started off at Bath Uni by Sara. biorxiv.org/content/10.110…

We (@RyanCookAMR ) updated the number of complete phage genomes we can find Genbank .Passing 22 K. millardlab.org/bioinformatics…

Learn about GP3 project, tackling Pasteurella infection of salmon with phages, led by @TSPKU .With @bentpetersen, myself, @MarthaClokie , and multiple others, including @SMichniewski who just joined the team pasteurella.dk bpetersen.dk/blog youtube.com/watch?v=uODSYS…

Busy day full of polymer science ahead! #GelPermeationCelebration2022 conference followed by the webinar, don't forget to tune in! #chemtwitter #polymerchem #AnywhereChem

So happy that @AmyGodfrey23 will be giving a talk at #Microbio22 🎉… She will be giving a talk on bacterial nanosyringes today at 12:15, be sure to check it!!

@SafeFoodCanuck Klebsiella pneumoniae

@SMichniewski Wonderful. What is the host?

One of our laboratory phages with as many as nine(!) different tail spikes (containing depolymerase enzymes). Due to their sheer number they create this weird bush or tree-like structure at the end. Photo done by Dr Saskia Bakker from University of Warwick.

@SaberTigerHP It belongs to myoviridae and it is indeed a broad spectrum phage.

@SMichniewski Wow, so many tail spike protein, maybe a broad spectrum phage? It looks like it belong to siphoviridae.

@dimiboeckaerts Rhys @RhysADunstan87 checked it via blast, hmmer, hhpred and phyre2.0 looking for conserved domains. He confirmed my 9 hits and added 1 more putative depo, ending with 10 potential candidates for protein expression studies. Rhys might have more details here :)

@SMichniewski Oh ok, interesting! I wonder whether they would all contain B-helices if you predicted their 3D structures.

@kadler42 p71

@SMichniewski Oh it's cute! Which is it?

@JulianGarneau Adsorption assay would be interesting indeed. The biggest issue would be obtaining the closest relative with less depolys (the closest relative at 98perc ident also encodes 9 depolys).

@SMichniewski Wow, that's impressive! I wonder how tail-fibers can still recognize and attach to their host through all that bush? Do they attach as efficiently thant their close relatives without depolymerases?

@dimiboeckaerts Blast search pointed to 9 putative depos. Host range analysis vs 150 klebs strains with various capsule types resulted in few halos being observed. Some halos could be confirmed via phylogenetics vs known phage depos. However, they still need to be expressed and tested to be sure

@SMichniewski Impressive! But how do you know they are all tail spikes (with enzymatic activity)?

@midlogphase @JessicaSacher @phagedirectory @PhageAustralia The only "almost lost it" case I ever had was when the cryotube opened during the transit by air. However, lots of parafilm and double layer transport container (tube-ception) contained the spillage.

@JessicaSacher @phagedirectory @PhageAustralia Has anybody been unsuccessful at shipping phage? They are generally so robust, that it takes effort to kill them.

Hey #phage community! What is everyone's favourite phage shipping temperature/buffer combo? (& what's the furthest you've shipped it successfully?) @phagedirectory @PhageAustralia

while visiting @DwayneR0ACH lab and some sunshine, @RyanCookAMR has updated complete phage genomes we can find in Genbank upto Jan1 2022. We have also reannotated all phage genomes using PHROGs and incorporated in INPHARED that can be downloaded. millardlab.org/bioinformatics…

through @MRCClimb I will be running a phage annotation and genomic comparison workshop later in the year (probably april). Poll below, to help us to decide on logistics if you are interested