Shivani Sachdev, PhD

Excited to share @Nature: How does naloxone (Narcan) stop an opioid overdose? We determined the first GDP-bound μ-opioid receptor–G protein (wt) structures and found naloxone traps a novel "latent” state, preventing GDP release and G protein activation. 🧵 nature.com/articles/s4158…

Evaluation of Alphafold modeling for elucidation of nanobody-peptide epitope interactions biorxiv.org/content/10.110…

Here to put the “boo” in boo-orthogonal click chemistry—and maybe pull a few tricks (or treats)! 🎃🧪 Happy #Halloween from the lab! #SpookyScience #LabHalloween

Degrader Tac-opedia 🔘PROTAC: E3 ligase-targeted proteolysis 🔘DUBTAC: Deubiquitinase stabilization 🔘LYTAC: Lysosome-targeted degradation 🔘Trivalent PROTAC: Multivalent E3 ligase 🔘AUTAC: Autophagy-targeted degradation 🔘RIBOTAC: RNA-targeted degradation 🔘RNA-PROTAC: RNA-binding E3 ligase 🔘BioPROTAC: Antibody/peptide proteolysis 🔘Aptamer-PROTAC: Aptamer proteolysis 🔘GlueTAC: Lysosomal traffic & degradation 🔘Peptide-PROTAC: Peptide-based E3 ligase 🔘NanoTAC: Nanobody-based E3 ligase H/T: Donggeon Kim on the HR app!

Nobel Prize is NOT about h-index or citations. It is about the emergence of big new fields. So many posts discuss Nobel awardees. And so many misunderstand the Nobel Prize. 📍 A bit of clarification from my side: 1⃣ Nobel Prize is NOT about how useful your work is. It’s about how useful it WILL BE. Science is not about real-world impact. It is about new knowledge, new understanding. It’s about nucleating new ways of thinking. Applications can come decades after the discovery. 2⃣ Nobel Prize is NOT about just doing risky research. Many of us take on risky projects. But most stay as niche studies that could have been done by others. It’s about doing what others are AFRAID to do. It's about looking like a reckless scientist. It’s about succeeding where others have failed (despite numerous attempts). 3⃣ Nobel Prize is NOT about a small study. It’s about nucleating a BIG research direction. It’s about "OMG, I didn't know it's even possible!" Yes, sometimes it takes decades to recognize a scientist. But in many cases, the prize was given to those who published the "nucleating studies" and pushed hard to grow the new field. 4⃣ Nobel Prize NOT about a lot of citations. Metrics doesn't matter. Forget this "Stanford top-2% ranking". Your peers' opinion is what really matters. Are you recognized by your peers SO MUCH that they want to see you as a Nobel Laureate? Do they see you as someone who created their field and made their research possible? Do they see you as their thought leader? ▫️ My HUGE congratulations to all Laureates. AI has changed science a lot. Those who made it possible deserve this recognition. #science #AcademicChatter

Congratulations to the 2024 #NobelPrize in Chemistry winners, Drs. David Baker, Demis Hassabis and John M. Jumper! Dr. Baker’s #NIH-supported work at @UWMedicine building new kinds of proteins is an incredible example of the value of taxpayer investment in biomedical research.

So she was first author on his key paper - in fact she's on most of his key papers, yet she didn't get the Nobel Prize, in spite of the fact they could have given 3 awards, not just 2. Explain to me how STEM is not institutionally sexist.

Excited to share new work from our lab led by Uyen Pham – “Location-biased β-arrestin conformations direct GPCR signaling.” We demonstrate that b-arrestins display distinct conformations in different subcellular locations in regulating ERK MAP kinases. biorxiv.org/content/10.110…

Cryo-EM structure of GPR179, a GPCR crucial for visual signalin, reveals how its dimer architecture fits curved membranes . @KLabGPCR nature.com/articles/s4146…

👩‍🔬 👨‍🔬 Join us for the 4th Transatlantic ECI GPCR Symposium this Thursday and Friday! Come for science done by ECIs, ask about current & future trends in lunch sessions, hear career panel discussion, and network! Last hours for registration: lnkd.in/dZ_NxBNa

Now published in @PLOSBiology: How GRK kinases control #GPCR signaling in the #Hedgehog transduction cascade in primary #cilia. journals.plos.org/plosbiology/ar…

My lab has multiple openings for those interested in doing some very cool science in beautiful Colorado! Research assistants (tech/lab manager level): cu.taleo.net/careersection/… Postdoctoral fellows: cu.taleo.net/careersection/… DM or email me if interested and 🙏 share broadly 🪃

New from Pharmechanics, a YouTube-based workshop on analyzing kinetic signaling data for GPCRs. Watch and learn how to do curve fitting on GPCR time course signaling data. youtu.be/m1WT9Mmso7Q?si…

Had an amazing last week in NH at #GRC phosphorylation and G-protein mediated signaling networks. Great science, engaging talks, and new research ideas. A huge thank you to the #NIH Fellows Award for Research Excellence and the Cheloha Group at #NIDDK for making this possible!!!

Wow--mind blown! "Computational design of soluble and functional membrane protein analogues" @BShoichet @RothLabUNC nature.com/articles/s4158…

Not my usual outside the🪟view!!! Enjoying amazing talks and stunning views at #GRC phosphorylation and G-protein signaling networks 🧪🏞️

Authors @rche54, @shivdoesscience and colleagues demonstrate that tethering ligands to GPCR receptors via conjugation with binding nanobodies enables pathway-specific signaling nature.com/articles/s4146…

@BadTransient @NatureComms Thanks, Chris!!!

Super excited to see this out!!! Published in @NatureComms (and open access!)🔥 Highly Pathway Selective #GPCR Signaling via Nanobody Tethering🦙💊 A 🧵