Tanner Fadero

We are excited to announce the release of @manager_micro v2.0.2_20230815, which includes a new feature in the “On-The-Fly Processor Pipeline” menu: Deskew! Broadly, this is useful for automatically deskewing, rotating, and projecting light sheet images in Micro-Manager. (1/7)

Magnetically-controllable antibodies!!!!! 🧲💉

I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!

Alright, I'm calling it. I'm over to the other place (rhymes with clue guy). Find me at my new profile: tanner dash fadero at bee ess kay why dot social dot com.

We present long-term stabilization of an oblique plane microscope (OPM). By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift. The feedback signal comes from a laser, no fluorescence is expended. biorxiv.org/content/10.110…

@JLazzariDean @Nano_Juli @AndriyChmyrov @AndrewGYork @amsikking I have independently used the cross brace and can attest to how well it works! Here's 8 hours of footage of O2's focal point on O3's surface. The room temperature drifted by ~1 C. What you don't see is the spot defocusing!

@Nano_Juli @AndriyChmyrov @AndrewGYork @amsikking has been tackling thermal stability a lot recently. He's posted some of his data, which suggests that the monolithic block could be a good approach. I think he's planning to try out monolithic blocks soon, so stay tuned! x.com/amsikking/stat…

At the heart of every snoutscope is a sub-micron alignment between two objectives. 1°C thermal drift kicks the lightsheet out of focus and destroys your image ☹️ Our optics room swings by 5°C/day 😭...so @AndrewGYork & I made a crossbraced O2/O3 subunit to passively compensate!

@aandr314 @Thorlabs @aandr314 that's my fault, I just bought their entire catalog

Hi @Thorlabsit seems that everything on the website is 3-5 days away?

Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snouty microscope! They have a great team there with @nico_stuurman, @KariHerrington and @micaelalasser (Instagram). A great location!😀

@RetoPaul (pi is not actually 4, the above graphic is a joke)

@RetoPaul yeah and pi = 4

Since some people are jonesing for distraction: Fun with discrete math.

PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.

And thanks to @amsikking for running a thermal calibration on this exact King Snout lens!

#snoutclub @UCSF

@aandr314 Huh weird. I think that's just a reflection but I'll check it tomorrow. Only thing it's seen is a < 5 mW laser

@TanFad Did you burn the iris

how I label my hex keys

@WallaceUcsf @Thorlabs uses red for their metric balldriver handles and yellow for their imperial balldrivers, yeah

@TanFad Wait is one color metric the other imperial?

Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all: beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/ Feedback always welcome. x.com/JamesDManton/s…

@A_CharlesOrszag Is it self-tanning if Tanner applied it?

Made a prototype light trap out of aluminum shim stock and Musou black paint. Watching paint dry has never been so interesting. The matte finish is fantastic!

@richardfuisz youtu.be/oKOC3Z9tDiI?si…

@richardfuisz Yep. You should use an airbrush for best results, but it's still pretty black even with a paintbrush.