Jan Becker retweetledi
We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and > 1 cm long mouse bone 🐭🦴 (🧵 5/6).
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Jan Becker
202 posts
Jan Becker
@RunningPhoton
I am a runner & data scientist at ZOMP (https://t.co/lRvQQEWC4X). Previously postdoc with @KukuraLab (@KavliOxford) and PhD with @RainerHeintzmann (@Leibniz_IPHT).
Cambridge, England Katılım Kasım 2019
261 Takip Edilen243 Takipçiler
@JamesDManton Maybe someone has some cool data from astronomy and wants to give it a try?
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@JamesDManton Here just an example of the spokes target that I used in the original tweet many years ago.
Notice how the stopping is SNR dependent:
High = 23 iter. | Medium = 22 iter. | Low = 17 iter.
GIF
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Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all:
beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/
Feedback always welcome.
x.com/JamesDManton/s…
James Manton@JamesDManton
We (@AndrewGYork, @RunningPhoton, @microRussell and I) are still working on the manuscript describing our automatic parameter-free deconvolution approach, but we've made a Google Colab notebook where you can try it out without needing to install anything: beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/
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@JamesDManton It also depends on the object though!
Objects containing a wide range of spatial frequencies tend to require larger number of iterations to be deconvolved.
GIF
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@christlet Fact that it can be used as stop criterion for deconvolution is very interesting
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Jan Becker retweetledi
Pre-Christmas Fun! Look what some of our researchers got up to today. Huge congrats to the winning team. Well deserved guys! #tugofwar
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Jan Becker retweetledi
Single-protein optical holography biorxiv.org/cgi/content/sh… #biorxiv_biophys
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A nice tweetorial from @KukuraLab can be found here:
twitter.com/KukuraLab/stat…
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Our work related to simulating mass photometry is now available @ACSPhotonics:
pubs.acs.org/doi/10.1021/ac…
@KavliOxford | @OxfordChemistry | @UZH_en
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A yellow photon running through the forrest ... moving at a speed v << c 😅
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Jan Becker retweetledi
Enjoying day 2 of #FOM2023🔬with lots of light-sheet talks. Visit poster P1-G/3 to learn more about dual-view oblique plane microscopy (dOPM) and its application to high-content imaging of 3D cancer organoid models as part of the @MACH3Cancer CRUK Accelerator.
Oporto, Portugal 🇵🇹 English
Any idea what this animation could represent? #FOM2023 | @FOMconferences | @KavliOxford | @OxfordChemistry
Come to my poster (P2-F / 11) in the "Various Topics" poster session on Tuesday to find out. 🔬💻👩🔬
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Jan Becker retweetledi
Further work from my PhD out in @SciReports : nature.com/articles/s4159…
With Hugh Sparks, @liliana_brito91 , Ken MacLeod,
@sian_harding, and Chris Dunsby
Many thanks to @ImperialPhysics @impchemistry
@icbcdt @NHLI @EPSRC @TheBHF
A mini-tweetorial (1/6):
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This study involves the simulation of the interferometric nature of the detected signal, a quantification of the effects of glass roughness and protein shape/orientation ... and many more.
(2/2)
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Our preprint on the principles & limitations on inferring mass from optical signals is out today.
biorxiv.org/content/10.110…
Many thanks to @KukuraLab at @KavliOxford | @OxfordChemistry and the Schuler lab @UZH_en
(1/2)
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Jan Becker retweetledi
Study from my PhD work recently out: doi.org/10.3389/fphys.…
With Hugh Sparks, Ken MacLeod and Chris Dunsby
(1/2)
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@adam_hanninen @Maurice_Y_Lee @TakahiroDeguch1 @AlexanderJugler @JonasRies @HeintzmannLab That is certainly correct, only that you would have to "shrink" it along the y-axis as you would detect less photons.
Resulting in the curve always residing below the green one.
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@RunningPhoton @Maurice_Y_Lee @TakahiroDeguch1 @AlexanderJugler @JonasRies @HeintzmannLab The OTF looks like a Schwarzschild- Cassegrain, also with a center obscuration
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Out today: As it turns out, imaging using a full pupil is not always the best one can do. Instead split the information & computationally recombine!
opg.optica.org/optica/fulltex…
Many thanks to: @TakahiroDeguch1, @AlexanderJugler, Ronny Förster, Uwe Hübner, @JonasRies, @HeintzmannLab
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