Yuriy Baglaenko

I recently had the opportunity to present our work at the Variant Effect Seminar Series. See a recording below if you're interesting in learning more about our recent published paper and on-going projects. youtube.com/watch?v=LPUV6b…

We recently pushed an updated to our protospacer Base editing design tool (baglaenkolab.shinyapps.io/snpGuide/) to allow for genome modification near a variant regardless or transition. If the region is important, so are the nucleotides around it. Hope this helps somebody out.

At PRES2025 in Helsinki to talk about multiomic single cell sequencing in juvenile arthritis. HMU if you'd like to chat about anything at all. #PRES2025

Woohooo!

Woohoo! Good job Dylan. Can confirm that TCAT works well on other datasets besides those featured in the paper.

@Nature Author here to answer any questions one might have about this work. The full protocols is on protocols.io and we have deposited codebooks to github. DMs open

Nature research paper: Precisely defining disease variant effects in CRISPR-edited single cells go.nature.com/3ILsmgw

@CincyResearch @Nature Happy to answer questions if anyone is curious to learn more.

Excited that @YuriyBaglaenko the first author of this paper @Nature about CRAFTseq methodology has since joined #CincinnatiChildrens Division of #HumanGenetics. Here's his bio page: cincinnatichildrens.org/bio/b/yuriy-ba…

Many many thanks to @soumya_boston, @ZepengMu, and every other co-author on this project. We are continuing to scale and implement these strategies in my lab, so reach out if that interests you.

Happy to answer any and all questions about this work. We'd love to see more people try it. Full protocol: protocols.io/view/craftseq-… Full analysis: github.com/immunogenomics…

I am very excited to share our publication, out now in Nature, detailing our developed CRAFTseq methodology for capturing and analysing single cell genomic DNA amplicons alongside the transcriptome and cell surface protein expression. #Sec8" target="_blank" rel="nofollow noopener">nature.com/articles/s4158…

My lab thinks a lot about CRISPR editing variants of interest in primary human cells. We made a little tool to help. Hopefully, someone else can get some use out of it. baglaenkolab.shinyapps.io/snpGuide/ github.com/ybaglaenko/snp…

@michecurtis and I are thrilled to share our preprint "Reproducible single cell annotation of programs underlying T-cell subsets, activation states, and functions" from the @soumya_boston lab @BrighamResearch and @broadinstitute! biorxiv.org/content/10.110… 1/12

How to pick a problem. Today in @CellCellPress. 1/53 cell.com/cell/fulltext/…

I need to remember this tweet

Better yet, please try out our PE7 editor for yourself! One of our plasmids is already available at @Addgene addgene.org/214813/, with more to come.

@soumya_boston @broadinstitute @MassGenBrigham Too many people to thank to count. I welcome any direct questions. If you want to discuss CRISPR editing in primary immune cells, shoot me a DM.

@soumya_boston @broadinstitute @MassGenBrigham The protocol is live. No specialized equipment required. Only the ability to sort single cells. Go check it out and send us your questions. protocols.io/view/minecraft…

CRISPR editing is hard. Editing in primary human cells is even harder. Editing non-coding variants in human cells is downright impossible. I am happy to share our work aimed at addressing this problem with @soumya_boston @broadinstitute @MassGenBrigham biorxiv.org/content/10.110…