ZO_lab

#AlphaFold2 worked for @zo_lab too! Orange – AF2 model, blue – X-ray structure (not in PDB). The transmembrane part of the helix was removed in the expression construct. (1/2)

Validation of 3D cryoEM single particle reconstruction correctness and handedness with Ewald's sphere correction biorxiv.org/cgi/content/sh… #biorxiv_biophys

Two @utswnews labs published articles in the same issue of @ScienceMagazine this week. Congrats to @DJSiegwart and @MishraLab 🎉🎉🥳👏👏

periodic reminder that I'm on the editorial board of Journal of Virology and you can suggest me as a reviewer. I almost never get asked to review for JVI (the fact that I haven't made a lab website yet may be part of it) but I like to think off myself as a decent reviewer...

We always thought nucleotide state strictly defined AAA+ conformation, but Jeff Mindrebo's work on mito LONP1 suggests more is at play - substrate interactions also drive protein translocation! Revisiting & rethinking the hand-over-hand mechanism: tinyurl.com/49y7a6h6

Happy to see it on bioRxiv @ZO_lab Structural architecture of TolQ-TolR inner membrane protein complex from opportunistic pathogen Acinetobacter baumannii biorxiv.org/content/10.110…

Kicking off the 2024 Cold Spring Harbor CryoEM course @cshlcourses! It’s going to be a grueling but amazing 2 weeks!

Proud to share our new paper with the Cramer lab (@mpgpresident) in @NatureSMB! Led by the wonderful @KokicGoran and @George_Yakoub_M with great collaborators. Elongation factor ELOF1 stably positions repair proteins on RNA polymerase II. nature.com/articles/s4159…

1.46 Å performance at JEOL demo lab! Researchers at JEOL in Japan installed Apollo on their microscope and generated the highest resolution (1.46 Angstrom) CryoEM data they had ever achieved on their scope, which was previously equipped with a Gatan K3. directelectron.com/apollo-achieve…

I am so excited to share that I will be starting a lab in January 2024 as an Assistant Professor at @OHSUSOM in Portland! I am building my team, so if you are interested in cellular cryoelectron tomography, computational structural biology, and working in PDX, hit me up!

After NU-refinement with Class 0 (112,303 ptcls), we got 2.91 A map. Here is the processing result.

We have made RFdiffusion free and open source for everyone! Please go check out the GitHub, and the Google Colab! And have fun designing with it!!

Today at 4pm at EHSEB Alumni Hall, don't miss Dr. Daniel Rosenbaum's talk on the molecular mechanism of mammalian cholesterol homeostasis!

Happy Monday! On March 30th, we will be hosting the amazing Dr. Lindsay Case! She was a postdoc at UTSW in the Rosen lab, where she received the Brown and Goldstein award for research excellence, and now has her own lab at MIT. We hope you can join us for this exciting event! 👏🏼

In the study Timm Koller @Timmeeeyyyy determines a structure of myxovalargin on the ribosomal large subunit at 2.1A resolution using single particle cryoEM

Last night I captured an incredibly difficult shot: ISS transiting the moon, while gently kissing Tycho crater as it flies by at 5 miles per second. That crater is 53 miles wide, so while the station almost looks like it's orbiting the moon it's actually 1000x closer to us.

It was fun showing coot 1.0 at #CCP4SW 2023

Iceland after the coldest December since 1974: @CopernicusEU’s Sentinel 3 satellite captured this rare almost cloud free white snow-land on 1 January #iceland #remotesensing #geography #winter #snow

@YunsunNam @Ruiz_Arroy0 Congratulations!

How does METTL1-WDR4 create m7G in tRNAs? We present a molecular movie of the methyltransferase in action through multiple #CryoEM structures. Work led by @Ruiz_Arroy0 in the Nam lab! nature.com/articles/s4158…

For the 3DVA and protein flexibility lovers (2/2), Check out the preprint for more details: biorxiv.org/content/10.110…. @MMSB_Lyon. Great work with Pavel Afonine and Billy Poon. Big thanks to @cryosparc for sharing some 3DVA maps.