Diane Brache

*New Paper* investigating sediment grain size impacts on #seagrass physiology, microbial communities, and sulfide porewater dynamics! Check it out here! link.springer.com/article/10.100… @estuariescoasts @bodegamarinelab @clurmurph @KatieDu_Ecology

@luisarchaeota @gavinjknott @MonashBDI @MonashUni Woohoo! 🎉 you’re future is bright

Thrilled to share that I'm joining Gavin Knott's lab (@gavinjknott) at @MonashBDI, @MonashUni as postdoc to explore Archaea-MGE arms race using genomics, biochemistry, and structural biology. Excited to join Monash's vibrant academic community! Let's connect if you're around – looking forward to it! 1/n

@tahirOleMiss I will look into it. Thx!

@diane_brache Try changing the composition of the media to get rid of these aggregates. Check if you have any salt in the media.

How does one get OD reading when your bacteria grows like this? I have several actinos from eelgrass sediments that do this.

@cosmic_bugg This might work for prepping glycerol stocks. I'm trying to get a good reading in the plate reader.

@diane_brache May be you can use small sterile ceramic/glass pieces during inoculation and incubate them on atleast 120rpm

@Talaumasearcher This has been suggested a few times. I'll look into it.

@MIriam__KRO They are very durable and bouncy

Xtra info: marine broth in shaker incubator @ 28C. I will have her try some different media. Wondering if switching to plaque assay may be better? I'm helping her cultivate her love of phages while keeping her project in sync with my own work. Phages are not my wheelhouse.

I have a UG student who wants to monitor the growth of the bacterial culture before and after the addition of Mitomycin C to induce prophages. We have 17 actinos I isolated and about 30% make these adorable colored spores.

@sacrebluecarbon Yes, I’ve tried using sterile cotton through a syringe after vortexing in pure water. This does break them up into a homogeneous suspension. BUT, I have an undergrad who needs to do growth curves in a plate reader with it. They will keep growing as balls under those conditions.

@diane_brache Can you filter it with a larger pore size filter (smaller if you want to remove more or all of the particulates)?

@lesliedayly Interesting, I have homework to do. Also, I still have your hat and mittens. 😬

@diane_brache Idk about actinos but I know for other organisms changing the salt content of the media can help it grow more planktonicly. V cute regardless

@AntonioTinocoV Happy Birthday Antonio! Pórtate mal, cuídate bien. 😜

Me, a grown 33 year old man on his birthday: My dad on the phone: “Hola mijo, felicidades y feliz cumpleaños. Te quiero mucho y PORFAVOR PORTATE BIEN!” 😂😂💀 I’ll never escape the “please behave and be polite” rant from my immigrant Mexican parents no matter how old I am 🤣

If becoming an NSF GFRP recipient myself wasn't already amazing, my dope labmate @MacvittieSophia also is a recipient. What an amazing day for #SoginLab ! 🎉

Mi Gente, I'm an #NSFGRFP recipient. Wepaaaa! Thx to @MaggieSogin for your guidance & to my LORs. To the undergrads @MDCollege YOU BELONG in STEM, my UG research there paved the path to PhD @QSB_UCMerced I am proof that public ED lead to upward mobility #latinasinSTEM

@Cerebro409 @QSB_UCMerced Congrats Tyrome 🎉🎉🎉

Today is the day 🥵😮‍💨

@GinaMChaput It was a fabulous experience to work and learn with you. 💪🏽

So proud of what @diane_brache & I accomplished for our first of four field sample collections!! As I said earlier it was a trial collection... If it all failed it was fine. First day we learned a LOT, made notes, changed some methods (1/2)

@yournameisvalid I’ve had colleagues with hyphenated names were the publisher messes the order of the name. Makes it difficult to search for their pubs. They end having 3 or 4 versions of their name which is a pain. It happens to me already at banks, credit card and dr offices etc already.

@diane_brache What's wrong with using your full name?

“Seagrasses occupy less than 0.2% of the seafloor but represent up to 10% of the ocean’s capacity to store carbon” m.huffpost.com/us/entry/us_60…