Gaublomme Laboratory

@eric_lubeck @TakaKud0 @ProCogia Advances in optical pooled screening to map spatial complexity #NBTNV go.nature.com/4gYuhuY

Also check out recent wonderfully complementary approaches PerturbView by @eric_lubeck and Aviv Regev, and NIS-seq by @schmidburgk

We’re looking for postdocs to leverage CRISPRmap to do pooled spatial genomics perturbation studies in vivo. Open to experimental and computational scientists. Please reach out at jg4106@columbia.edu!

Excited to share: "Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap", just published in @NatureBiotech. Spearheaded by @Jiacheng_Gu_, and powered by everyone in the @GaublommeL at @Columbia_Bio and collaborators. tinyurl.com/4zx95v3a

Mapping multimodal phenotypes to perturbations in cells and tissue with CRISPRmap go.nature.com/481iCHt

We’re looking for postdocs to leverage CRISPRmap in pooled spatial genomic perturbation studies. Open to experimental and computational candidates. Looking forward to discuss the opportunities! Please reach out at jg4106@columbia.edu!

First author @Jiacheng_Gu_ didn't have to adjust the CRISPRmap protocol reported on biorxiv for barcode readout in these cell types. biorxiv.org/content/10.110…

Update: for those interested in optical pooled screening in stem/pluripotent cells and neurons, we tested CRISPRmap in hESCs, iPSCs and iPSC derived neurons, and observed good barcode recovery. Shout out to the Wichterle lab and @SnoeckLab for their help in these cell contexts.

We’re looking for postdocs to leverage CRISPRmap to do pooled spatial genomics perturbation studies in vivo. Open for experimental and computational folks. Looking forward to discuss the opportunities! Please reach out at jg4106@columbia.edu! Retweets appreciated!

Excited to share: "CRISPRmap: Sequencing-free optical pooled screens mapping multi-omic phenotypes in cells and tissue". We applied our novel approach in wonderful collaborations with @CicciaAlberto, @DrEdmondChan, @elhamazizi, and @landau_lab. biorxiv.org/content/10.110…

Also check out a recent wonderfully complementary approach by @eric_lubeck and Aviv Regev! biorxiv.org/content/10.110…

Try CRISPRmap for your own research goals! Reagent costs associated with barcode readout are quite low (~$80 per million cells), readout dyes can be matched to your existing microscopy setup, and we're happy to provide some initial reagents for you to get started.

Gaublomme and Azizi labs at Columbia are looking to jointly hire a computational postdoc. Any referral of talented people that might be interested would be greatly appreciated! pa334.peopleadmin.com/postings/5093