Jane Chen

@RachelGatlin5 Now is a good time to start looking at what might be of interest and network, but realistically apply ~3-4 months out bc most companies will not hold a position open for a year.

Our in vivo deep-brain fluorescence recording (photometry, miniscope, multiphoton) primer is finally out! Many thanks to Adam Gordon-Fennell, @PiantadosiSean , @NaJiBerkeley , @Labrigger , @mbruchas , @gstuber for sharing expertise and best practices. authors.elsevier.com/c/1htVZ3BtfH5M…

@neurovika @johnfneumaier @gstuber @NIH @NIDAnews Amazing! Congratulations!!! 🎉🥰

Super excited to share that I received the 3-year NIH F31 predoctoral award! I am endlessly grateful to amazing PI Sheri Mizumori, my mentors (@johnfneumaier, @gstuber, Larry Zweifel, Michael Bruchas, Jeansok Kim), @NIH, and @NIDAnews for giving me this incredible opportunity.

Impressive data out of the box ⁦@10xGenomics⁩ #Xenium. Thanks Dr. Simon Gregory at ⁦⁦⁦@dukemedicine⁩ 👏🏼👏🏼 #firstrun #spatial #translational linkedin.com/posts/simon-gr…

@sarah_stern Richard Palmiter’s lab has this, though I forgot who made it initially. Happy to help connect you to an aliquot.

Looking to try the cre-dependent TetTox virus ... doesn't look like it's on addgene .... if anyone has it, lmk so I can pick your brain (and/or ask for an aliquot)

@RachelGatlin5 Leftover company holiday gift? Secretly smuggling counterfeit things? 🤷🏻‍♀️😅

@RachelGatlin5 Much more pleasant to unwrap than dead cats….

Completed our first Xenium experiment with a custom panel and it was a huge success!  Ran two slides with multiple regions selected based on histology and previous Visium data. Here are the highlights of our run including region selection and our first dive into the data.

@iamhans @dimacdonald @SekunP @neuron_MY Took a while to dig up my notes on this. We did not look at 40 Hz stim, but did test 30 Hz on a few cells (<5 and only a few pulses, not enough to draw any conclusions). Definitely would be interesting to look at!

@dimacdonald @SekunP @neuron_MY @jane_y_chen Thanks very much! We haven't done it (@jane_y_chen can confirm it), but I am also curious to look at it. Mice with vglut2 deletion showed no behavioral changes during threat learning. So, I guess that neuropeptide release without glu may be sufficient to convey the US.

New preprint from the lab in collaboration with the Palmiter lab and Zweifel lab at UW! We developed two novel genetically encoded tools: Neuropeptide release sensor & silencer that works from PRESYNAPTIC TERMINALS in behaving mice. 1/5 biorxiv.org/content/10.110…

The UW-SOAR Program offers a two-year research opportunity in NAPE labs for recent graduates from diverse backgrounds. Applications are open! Learn more here: depts.washington.edu/uwnape/trainin…

Breaking 🗞: The first Xeniums are heading out into the world! We can’t wait to see what insights they uncover. Want to level up your research? See how Xenium lets you map 100s of RNA targets with subcellular resolution & reserve yours today: 10xgen.com/3YaXhG6

@sarah_stern PBN CGRP neurons :)

Seeking for a proof of principle experiment: A genetically-defined population of neurons in which there is cfos activation by some stimulus AND a known downstream projection. If you have any good candidates, please let me know :)

We also wrote a preview on a study by Xie et al that identified a gut-to-brain circuit underlying retching behavior. This plays an important role in coordinating responses and learning to avoid foods that made animals sick. authors.elsevier.com/c/1g3VbL7PXiqY7

Using this, we were able to identify spatially distinct gene expression changes in the PBN after chronic pain. This incredible work was led by @GuLiangcai, Xiaonan, Li, and Runze and contribution from @LoganCondon2. Looking forward to more applications of Pixel-seq in the future!

I’m so exited to share that Pixel-seq has been published! We describe a stamping method to scale production of polony gels with <1 um resolution for #spatialbiology and a clustering algorithm to segment cells based on their UMIs and transcript similarity. authors.elsevier.com/c/1g3VXL7PXiqZk

3.5 month update: PBN paper has been accepted at @eLife! elifesciences.org/articles/81868. Stay tuned for another exciting paper coming very soon.

@pastramimachine My PI gave everyone in the lab a Christmas bonus each year, funded by all the royalties he received from talks, distributing reagents that he cashed out from his own bank account.

Do any PIs explicitly share “profits” from successful grants with trainees? I.e., pay bonuses for grads if grant hits?

🚨neuro postdocs! UCLA is starting an extramural postdoc seminar series (SYNCS)! 🥳 we are so excited to bring a diverse group of postdocs to visit! applications due Oct 3. apply!! application link: tinyurl.com/uclasyncs

@_Chen_Ran_ Nice work! Looking forward to reading this :)

How does the brain differentially encode signals from the visceral organs to generate our internal senses? My postdoc paper @Nature reports the coding of organs by Ca imaging and reveals a visceral homunculus in the brainstem that depends on inhibition. nature.com/articles/s4158…