Jacob K

Check out our new study where we find that the p97-UBXD8 complex regulates ER-mito contact sites by regulating lipid desaturation nature.com/articles/s4146… Congrats to first author @Rak35hG @TuftsGSBS @TuftsUniversity @TuftsMedSchool @GygiLab @LabWeihl

@JayXiaojunTan @TrendsCellBio Fantastic review! I'm excited to dive into it.

Lysosomes are essential for cellular quality control, but how about the quality control of the lysosome itself? Online now @TrendsCellBio, we reviewed the molecular mechanisms of four major lysosomal quality control pathways: TFEB/Lysophagy/ESCRT/PITT. authors.elsevier.com/a/1gVEr3QxxSr6…

@DrlxReina At a quick glance it seems like all the wells with the foam at the top aren't running. I'd check to make sure there's enough buffer to cover all the wells which should displace the foam. My bet is that the foam is hindering conductance which is why those samples aren't running

Hello! What would cause my gel to run like this? Is the cassette bad? (The other gel is running just fine! )

Great day at the Tufts MD-PhD retreat, always amazed by these talented students @TuftsMedSchool @TuftsGSBS @jay_clicks

Today is #WomeninScienceDay. I have a lab with amazing women I’d like to highlight today. Kicking it off with my lab in #steminist t-shirts. We have 2 amazing guys as well @jay_clicks @RakeshG91824202 who gamely wore the t shirts in honor of today.

@IrisMontes19 @noellneuro TBH people panic and buy out all the bread and milk when there's less than a foot too. But I'm not going to work tomorrow... Pretty sure the T will be closed and roads undrivable

@noellneuro I wasn’t worried until I saw the panic in the grocery stores 😅

I’ve lived in Boston since 2018. Wondering if I’m not taking this blizzard warning as seriously as I should since I’m still planning to go to lab tomorrow. who’s laughing at me #islandgirl

@D_HellerTrulli Hmmm, adding more classes to burnt out residents didn't help. Surprising. Maybe if they add mandatory yoga sessions after overnights🤔🤔🤔

Check out a new paper from the lab! New role for p97 at organelle contact sites: The p97-UBXD8 complex modulates ER-Mitochondria contact sites by modulating membrane lipid saturation and composition biorxiv.org/content/10.110…

The p97-UBXD8 complex modulates ER-Mitochondria contact sites by modulating membrane lipid saturation and composition biorxiv.org/cgi/content/sh… #bioRxiv

@avtytla Isn't it based on surface area instead of volumes? IE 1:3 pass is taking whatever cells are in 1 cm^2 and putting into 3 cm^2. So for replating into the same dish, it's 1/3 of the cells but if replating into a dish 3x the size, use all the cells. This is wrinkling my brain

@oldladyscience Were just not photogenic enough to be featured 😭😭

Versanis Bio, founded and seeded by Aditum Bio, raises $70M Series A financing led by @atlasventure and @medicxi to advance bimagrumab into an advanced Phase 2 program for the treatment of obesity. businesswire.com/news/home/2021…

Every year 17 million people die from neglected surgical conditions. Surgery is a necessary part of Universal Health Coverage. Sen @ChrisMurphyCT we need your support! #SurgFundUSAID #FundSurgery #globalsurgery #globalanesthesia #globalobstetrics #safersurgery #SurgicalPlanning

Sen @chriscoons, I am writing to seek your support in our requests for increased global health funding in the FY22 US federal global health budget which would improve surgical safety on a global scale. globalsurgerystudents.org/usaid #SurgFundUSAID #globalsurgery #fundsurgery

Where are the Boston fireworks? No sign of them

@pablo_jack @AnoopArunagiri DM'd for troubleshooting!

@jay_clicks @AnoopArunagiri Indeed! I think that only icy software has something to measure dispersion. BTW, I tried the macro but I found some problems with line182 and with bioformat line when I choose setup.

AggreCount: an unbiased image analysis tool for identifying and quantifying cellular aggregates in a spatially defined manner #proteinaggregation #proteostasis #proteinmisfolding #misfolding #ImageJ jbc.org/article/S0021-…

@pablo_jack @AnoopArunagiri That's an interesting challenge. AggreCount can capture ROIs of nuclei, cell borders, and puncta. Theoretically it could calc the distance between cell borders and puncta using the same methodology it uses now for nuc->puncta distance.

@jay_clicks @AnoopArunagiri Oh that is great! I was waiting long time for something like this. Now I am trying to find a way to measure aggregation from nucleus to cell periphery, as a distance map for puncta dispersion. Maybe aggrecount 2.0 in the future :)

@pablo_jack @AnoopArunagiri Absolutely! We actually started using it to quantify peroxisomes in our lab. In its essence, AggreCount isolates and quantifies bright puncta and then measures the distance between the puncta and a nucleus. So anything with punctate staining may be quantified. LMK how it works

@AnoopArunagiri This is great! DO you think that I can adjust the parameters and use it to quantify vesicles or organelles accumulation?