Judit Villen

Very proud of fearless graduate student @alexischang3000 for facilitating HT phosphotyrosine studies through this method.

How cells age? How they remain young? How they rejuvenate? We were amazed on how combining the multiple proteomic assays can yield mechanistic insight into the basic biology of aging , e.g. regarding asymmetric division, organelle partition, and trajectories of protein complexes.

Excited to share our newest #preprint: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation. A great project with the @juditvr @DunhamLab @mkaeberlein teams! 📄Read: biorxiv.org/content/10.110… 💾Explore: rlsproteomics.gs.washington.edu 1/9

Happy to share new preprint from superstar postdoc @MarioLeutert in my lab. One of a kind phosphoproteomic study (101 perturbations x 6) enabling cool analyses and novel discoveries in signaling. The regulatory landscape of the yeast phosphoproteome biorxiv.org/content/10.110…

Mistranslation + biochem assays + MS = variant interpretation across the proteome. Innovative idea by @RRodriguezMias and co-developed with @noncanonikyle, and many other contributors from Villen, Fields and Noble lab. MiRo: MistranslationRocks

I am very excited to share the latest technology from my lab. Proteome-wide identification of amino acid substitutions deleterious for protein function biorxiv.org/content/10.110…

I am missing #ASMS2021 this year. But several cool presentations from students and postdocs in my lab on phosphoproteomics, MS acquisition methods, and functional annotation of protein variants @AnthonyBarente @MarioLeutert @AlexHgO @smithianr @noncanonikyle

Excited to share our review on PTM crosstalk @molcellprot with @sam_entwisle @juditvr ! We discuss the basic modules of PTM crosstalk and focus on emerging technologies to study PTM crosstalk proteome-wide! #Proteomics #TeamMassSpec doi.org/10.1016/j.mcpr…

"With science, numbers speak much louder than words." statnews.com/2020/05/19/vac…

@dlswaney @ProteomicsNews @uniprot @NCBI That is great Dani! Looking forward to learning from this data.

@ProteomicsNews @uniprot @NCBI We are almost done generating PPI map of SARS-CoV-2. I will be uploading to PRIDE in the next 2 days, which should be helpful for SRM assays.

My lab is looking for new postdocs and research scientists (B.Sc) interested in kinase signaling, EMT, cancer, and proteomics. Please RT!

The Center for the Multiplexed Assessment of Phenotype is excited to announce the launch of our new #Internship! This will be a unique opportunity to work with a team of scientists at the cutting edge of genomics and proteomics. Learn more at cmap.gs.washington.edu/outreach-inter…

01/2020 Issue --> embopress.org/toc/17444292/2… The cover (by @TeamSciStories) highlights two automated sample preparation methods for #phosphoproteomics -R2-P2 from @juditvr @uwgenome- & #clinicalproteomics -autoSP3 from J Krijgsveld @DKFZ- #proteomics

See also accompanying paper by @savitski_lab biorxiv.org/content/10.110…

Phosphoproteomics has catalogued 100,000s phosphosites. Can we pinpoint which are functional? We compare thermal stability of proteins and phosphoproteins to identify phosphosites altering structure and/or interactions. biorxiv.org/content/10.110…

New automated end‐to‐end phosphoproteomic sample preparation method for multidimensional #CellSignaling studies --> bit.ly/2Mf31LL from @juditvr @uwgenome #proteomics #phosphoproteomics #MSBmethod

@shaoenong Very exciting to see this put together. Amazing resource. Congrats!

Very excited to share our new preprint describing a pharmacoproteomics platform to identify cell signaling pathways and kinome biomarkers of drug response in liver cancer. So proud of work led by Martin Golkowski. biorxiv.org/content/10.110…

Thesaurus, from @juditvr and colleagues: a new proteomics search engine that finds phosphopeptide positional isomers from PRM and DIA mass spec data. nature.com/articles/s4159…

@Lange_Lab @MarioLeutert @RRodriguezMias We are not there yet, but peptide IPs for phosphoTyr, and other PTMs are in the pipeline.

@juditvr @MarioLeutert @RRodriguezMias Do you have something similar for tyrosine in the making?

New preprint from the Villen lab: R2-P2 method for automated phosphoproteomic sample preparation from cell lysates and application to MAPK signaling; work led by @MarioLeutert with contributions from Noelle Fukuda and @RRodriguezMias biorxiv.org/content/10.110…

Facilitating phosphoproteomics analysis of hundreds of samples with robust and reproducible high throughout sample prep.