Michael Steidel
494 posts

Michael Steidel
@michaelsteidel1
#Cellzome #TeamMassSpec opinions are my own
Heidelberg Katılım Eylül 2021
342 Takip Edilen240 Takipçiler


BREAKING NIUS: Fast 13 Milliarden Euro Kindergeld hat Deutschland 2025 an Ausländer oder ins Ausland überwiesen. nius.de/politik/news/d…

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Hey #TeamMassSpec
Proteomics Ruler question: there doesn’t seem to be a clear, explicit reference on what exactly to include.
Should the ruler be based on core histones only (H2A/B, H3, H4), or also include H1 (linker histones) - or even more?
Any solid references?
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Michael Steidel retweetledi

Solving the computational challenge of phosphoproteomics with 𝐏𝐡𝐨-𝐓𝐢𝐩: dephosphorylation on-tip identifies the sequences of phosphorylated peptides.
This serves as a basis for predicted spectral libraries, reducing the search space 10x-20x. biorxiv.org/content/10.110…

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By the way.. 43% of current TREMBL entries will be dropped soon anyway insideuniprot.blogspot.com/2025/06/captur…
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Hey #TeamMassSpec, Many non-human proteomics studies still search against taxon-filtered FASTAs.
❌ Redundant sequences
❌ Inflated search space
✅ Reference proteomes cut redundancy, improve annotation, and make results comparable. #proteomics #uniprot

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@DemichevLab Interface great as it is. Maybe makes sense to add an additional box to add data for library refinement only (no quant).
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Dear colleagues, would you like DIA-NN interface to change or stay similar? Would be grateful if you reply with your thoughts on this. The rationale behind the current interface is to enable quick work with multiple experiments, helped by:
- Multiple independent tabs.
- All settings visible at the same time.
Do you consider these properties important?
Or instead you'd prefer a pretty modern-style interface with step-by-step configuration of the analysis?
Any particular feature that is currently missing?
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Michael Steidel retweetledi

Wir brauchen gar keinen Druck zur #Remigration. Die meisten fahren doch aktuell freiwillig zum Urlaub in das Land, aus dem sie angeblich flüchten mussten. Wenn wir jetzt bei deren Rückkehrversuch konsequent die Grenzen kontrollieren und schließen, haben wir viel erreicht.

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Astral Zoom hits >7,000 protein groups & 67,000 precursors — on a 500 SPD EvoSep ENO run.
biorxiv.org/content/10.110…

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@heritage_lost Stell dir vor, du schaust ein Kind beim Lächeln zu – und spürst dabei… dich selbst.
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@Kachelmann Solche Tageshöchsttemperaturen sind heute natürlich völlig ausgeschlossen. Außer halt, wenn sie passieren

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Die Höchsttemperaturen vor 45 Jahren.
Solche Höchsttemperaturen sind in der modernen Klimawelt praktisch nicht mehr möglich.
kachelmannwetter.com/de/messwerte/d…
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#TeamMassSpec, Any opinions on why not generally adding the relatively small yeast proteome to the anyway large human search space (*.fasta) as an internal FDR quality control?
nature.com/articles/s4159…
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I regret some of my posts about President @realDonaldTrump last week. They went too far.
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Michael Steidel retweetledi

Protein digestion in 200 nL on solid-phase tips: SPEC enables low-input proteomics—even with SDS/SDC at high levels. From FFPE and muscle to high throughput plasma. SPEC is a universal and Evosep-compatible protocol.
biorxiv.org/content/10.110…
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