Philipp Hoess

Updated version of our figure visualizing different labeling techniques for SMLM after feedback from @UEndesfelder and @christlet. bivBC2 Nb is now divalent with a single dye (thx AlphaFold2) and 2ndary Ab is rotated to not exaggerate the displacement compared to labeled primary.

New tool for controlling (super-resolution) microscopes using an affordable FPGA by Joran, includes some multi-color and super-resolved images by me :)

If you happen to be at #CellBio2022 and are interested in how we reconstruct clathrin coat remodeling during #endocytosis using superresolution microscopy, come by my talk in Subgroup: Remodeling and Reshaping Membranes, Dec 7 at 09:14, Room 147A!

A new review by my colleague Michal about the protein machinery crucial for actin-driven endocytosis in yeast. Recommended reading! 📰

@HerzNiclas Hätte auch gerne eins!

Der Großteil des Erlöses verbleibt als Choreospende bei den Corillos. Um planen zu können brauchen wir aber verbindliche Vorbestellungen. Schreibt gerne hier in die Antworten, falls ihr ein Poster haben möchtet und retweetet fleißig. Ach ja; die Maße sind 90x60 cm. (2/2)

Liebe SCF-Bubbele wir, @tillseko1904 und ich hatten heute ein erstes Strategiemeeting mit den @Corilloultras wegen des DFB-POKAL Posters. Stand jetzt können wir es tatsächlich möglich machen, dass das Ding über die Corillo Ultras produziert und verkauft wird. (1/2)

MicroFPGA: an affordable FPGA platform for microscope control biorxiv.org/cgi/content/sh… #biorxiv_biophys

Original thread: twitter.com/UEndesfelder/s…

Corresponding review with the original figure: annualreviews.org/doi/abs/10.114…

Updated version of our figure visualizing different labeling techniques for SMLM after feedback from @UEndesfelder and @christlet. bivBC2 Nb is now divalent with a single dye (thx AlphaFold2) and 2ndary Ab is rotated to not exaggerate the displacement compared to labeled primary.

@UEndesfelder @ewers_helge @JonasRies But that would also mean to include the 2nd Nb to make it bivalent? I only found a structure for the single Nb, that's why I went with it. To be honest, I naively put the labels on surface lysines and was confirmed by Fig 1a in the NatComms paper, though not perfect for SMLM

@christlet @UEndesfelder @JonasRies The structure of the affimer is the basis of a library for phage display, maybe one could develop a MT-binding affimer based on it. 2/2

@christlet @UEndesfelder @JonasRies Some of the labels are actually illustrational and have not been shown experimentally on microtubules. We wanted to have a single structure showing many different labeling possibilities and the linkage errors they introduce. 1/2

@christlet @UEndesfelder @JonasRies Thanks for the feedback, Christophe. I totally agree that the effect of the 2ndary Ab exaggerates the displacement. In a static way it's not that easy to show, your suggestion with another Ab with 90° rotation is great. Unfortunately, we didn't do a review on Twitter before ;-)

@michellefrei13 Thanks Michelle!😊

I'm especially proud of this figure about linkage error I made using @dsgoodsell's Illustrate

I'm happy that our review about Super-Resolution Microscopy for Structural Biology is online now! Sheng Liu, @JonasRies, and me summarized the state of the art for Localization Microscopy and important aspects for structural studies. Check it out here: annualreviews.org/doi/10.1146/an…

Very excited to present my poster at #cellbio2021. If you are interested in clathrin-coat remodeling during endocytosis, drop by my poster today at 7:15 pm CET/1:15 pm EST!

DECODE is now published in Nature Methods! This deep-learning based method allows for localising dense fluorophores in 3D for SMLM with a high precision. nature.com/articles/s4159…

@yule60150484 in my lab developed LocMoFit, an algorithm to fit an arbitrary geometric model to SMLM data. It calculates the most likely parameters of the model and can even decide, which model describes the data better.

Check out these new dyes and their application in no-wash and super-resolution fluorescence microscopy. @aline_tschanz and me are happy to have contributed the localization microscopy part in @JonasRies lab!

Had a great time at this course in 2018, very good format to meet and interact with awesome people. And not to forget the food, cheese, wine and free time activities!