Ekaterina Bestsennaia

Last Friday, we wrapped up #EMBLFluorescenceLifetime 🔬✨ Thank you to all the participants, trainers, speakers, and organisers who made this course such a success! We hope you enjoyed it and feel confident to put your new skills into action! 🤝 See you next time! 🔵 Scientific organisers: Luis Alvarez, Leica Microsystems, Germany Manuel Gunkel, EMBL Heidelberg, Germany Birgit Koch, Max Planck Institute for Medical Research, Germany Andrea Müller, Leica Microsystems, Germany 🔵 Course organiser: Karo Wolkersdorfer, EMBL Heidelberg, Germany

During the 5 day #EMBLFluorescenceLifetime course, participants get hands-on training every afternoon on our state-of the art microscopes 🔬 Practicals include Halo-tags & FLIM on STELLARIS inverted DIVE, Multiplexing with FPs and FLIM on STELLARIS 8 STED, as well as FLIM analysis including ML and AI on STELLARIS Cryo 🤩

See you there bsky.app/profile/pricel…

@ScienceLinX 7 days before the #microscopiedag (Nov 9th) in Groningen & Drenthe! 70microscopen.nl Soap bubbles under the microscope 🔬🫧🫧🌈 🧵34/n @ScienceLinx #Microscopy

@alcien_calie Congratulations! 🎉

A good reason for the first post here

My happy chance to present a poster at @SMLM_Symposium (and enjoy late summer in Portugal 🌊) #SMLMS2024

Demonstrating super-resolution imaging and single-molecule detection with flippers in the latest paper of the Fürstenberg lab in collaboration with the Matile group @afuerstenberg @matile_group pubs.acs.org/doi/full/10.10…

The whole Flipper-TR family of mechanosensitive probe goes #SMLM & PAINT thanks to Jimmy Maillard, Ewa Grassin, @pricelesska and colleagues in a joint effort from @matile_group and Alex Fürstenberg labs in @sciences_UNIGE. Congrats! doi.org/10.1021/acs.jp…

Check out our new paper🎉 doi.org/10.1021/acs.jp… Mechanosensitive Flipper-TR from @matile_group can be used for #SMLM in model membranes: ✨Single-molecule tracking of Flipper-TR in lipid bilayers with varying fluidity ✨Super-resolution imaging of both Lo and Ld GUVs

📢#PaperAlert In our new paper we introduce GFP-inspired thiol-reactive fluorophores 🌟🌈 Their spectra sense ligand-induced structural changes in proteins 🧪🔬 #GPCR Our dye outperforms bimane, Cy-dyes & TMR💪 doi.org/10.1016/j.isci… @iScience_CP Warm thanks to all authors!

See you at @Sfb1348 meeting on Molecular Mechanisms of Membrane Organization 🙌

@elleelize what a great result, congrats! 🎊

My first first-author paper! A teamwork of many great researchers across different labs and countries. So cool to finally see it published:)

very relatable conclusions 🥲 from a great talk on the fundamental properties of fluorescent proteins (FPs) by @BourgeoisDom2 #fluorescenceleshouches

🌈 molecular gastronomy by @NathanShaner at the École de physique des Houches #fluorescenceleshouches

These are PALM movies of monomeric and dimeric membrane proteins labeled with mEos3.2 in fixed HEK293 cells ✨✨✨ doi.org/10.5281/zenodo… doi.org/10.5281/zenodo… doi.org/10.5281/zenodo… doi.org/10.5281/zenodo…

anyway, having mentioned data on Zenodo... If you are looking for raw #SMLM #PALM microscopy data to train localization or clustering algorithms, to analyze fluorescent proteins blinking, or for any other purpose, you might be interested in this! ⬇️

turned out my brain replaces 'date' with 'data', and this explains a lot

One small step for the rest, one giant leap for me! Happy and thankful to all involved 🥹

Channelrhodopsin-2 Oligomerization in Cell Membrane Revealed by Photo-Activated Localization Microscopy (Valentin Borshchevskiy and co-workers) @pricelesska @IvanMasl0v @alekseyka1993 @Aynya5 @dzabelskii @heilemannlab @BoshchevskiyV #openaccess onlinelibrary.wiley.com/doi/10.1002/an…

Nevertheless, there's some good news – our joint work with Dominik Wöll and Alexandre Fürstenberg is finally out. It demonstrates how we can utilize FL-SMLM for environmental sensing, specifically determining local water content in polymer gels. onlinelibrary.wiley.com/doi/10.1002/an…