Spirochrome

Spirochrome is on Bluesky! Don't forget to give us a follow when you make it there, we'll follow you back! 🦋🦋🦋

Thought about it but it was not sold now in US😂

@HJRBio Not anymore! cytoskeleton.com/product/hak-ac…

An amazing example of actin imaging with our expansion microscopy probe for actin HAK-actin™ These are U-ExM expanded platelets stained with HAK-Actin (magenta bottom), (anti-integrin αIIb magenta top), and fibrin (fibrinogen-A488, green). Movie source biorxiv.org/content/10.648…

HAK-actin product link: spirochrome.com/product/hak-ac…

Thrilled to be back in my home state preaching the gospel of Actin. Thank you @Rice_BIOE for the invitation to present my work!

🧵 1/5 A new BioRxiv preprint reveals that membrane tension acts as a mechanical gate for lung cell fate during development! 🫁 Our Flipper-TR probe was key to assess live Cell Membrane Tension (CMT) Here’s how Flipper-TR unlocked a hidden layer of lung development & fibrosis.

5/5 The Takeaway: Flipper-TR didn't just report CMT; it identified CMT as a master switch linking physics to genetics. From development to disease, this probe proved that how "tight" a cell is matters just as much as its DNA. Read the full study: biorxiv.org/content/10.648…

4/5 The "Stuck" State: Flipper-TR also exposed a fibrosis mechanism In healthy lungs, tension drops to allow repair. But in fibrotic lungs (IPF), Flipper-TR showed tension stays high This high tension state traps cells in a dysfunctional "transitional state," preventing healing

3/5 Using Flipper-TR + FLIM imaging, researchers visualized tension in live lung tissue. They discovered a drop in membrane tension before cells differentiate into AT1 or AT2 alveolar cells. Even cooler? It mapped "emergent zones" of low tension where differentiation starts!

2/5 🔬 The Challenge: Scientists knew various signals guided lung differentiation, but how do cells "know" when to specialize? Here comes Flipper-TR: A mechanosensitive dye that changes fluorescence lifetime based on CMT. High tension = Long lifetime Low tension = Short lifetime

🟢FastAct goes green! 🟢 We launch SPY488-FastAct a green channel live cell actin probe SPY488-FastAct allows imaging actin in live or fixed cells Combine with SPY555 + SPY650 for easy 3 color imaging spirochrome.com/product/spy488… Available at our distributors in a couple of days

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday Easy to use: just dilute the probe in cell culture medium and image 30 min later! 🔗 spirochrome.com/product/spy650… 🙏 Big thanks to @SaladinLazare for the images.

This is how actin shapes the neuronal growth cone 🔬 When a neuron grows, it doesn’t just extend blindly. It actively moves, explores its surroundings, and makes decisions along the way. #Neuroscience #ScienceCommunication #Microscopy #brain #Science #LifeSciences #neuron

The images were acquired on a @LeicaMicro Stellaris system by Armando Rubio Ramos from the Centriole lab in Geneva. Huge thanks for trying this out!

Are you staining DNA in your ExM samples? Great News: All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol. You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies. spirochrome.com/products/?_cat…

Peroxi_SPY probes were designed for peroxisome live cell imaging. Turns out they work with fixed cells as well! Peroxi_SPY can thus be combined with antibody labeling A new JoVe article provides a detailed protocol: app.jove.com/t/69405/peroxi… Products link: spirochrome.com/products/?_cat…

A new article by @ZhixingChen2 shows that the gentle & far red mitochondrial probe PKmito Deep Red is a robust mitochondrial membrane potential probe when imaged by FLIM. nature.com/articles/s4146… PKmito Deep Red is exclusively available at Spirochrome: spirochrome.com/product/pkmito…