Richard Held

My latest bit of work is up as a pre-print. We made an AMPA receptor GluA2 knock-in mouse in order to label and localize endogenous AMPARs at the synapse using cryoET. More details below and on biorxiv here: biorxiv.org/content/10.110…

@james_krieger Working on it and will update when they're up

My latest bit of work is up as a pre-print. We made an AMPA receptor GluA2 knock-in mouse in order to label and localize endogenous AMPARs at the synapse using cryoET. More details below and on biorxiv here: biorxiv.org/content/10.110…

A brief lull in work intensity to allow me to dwell in anxiety over the election.

@RaduAricescu Thank you! Pretty excited about it

@rgsheld Amazing work, congratulations!!!

@igreger1 Thanks!

@rgsheld nice!

Main change is a better movie 😬

Updated version of the preprint here: biorxiv.org/content/10.110…

@vikasnavaratna Thanks! Still lots to do

@rgsheld Congratulations Richard!! Way to go!

@jjanetzko Thanks!

@rgsheld Congrats Richard!

I'm excited to move forward with this work. After staring at cartoon schematics of the synapse for over a decade, this work convinced me that we can use that built-up knowledge to make real molecular maps and even atomic models of something as complex as the synapse.

We can tie it all together and look at how this maps back to our tomograms. AMPARs in complex with PSD scaffolds form dense clusters, with free receptors decorating the periphery, and docked vesicles around the cluster perimeter.