Rishwanth Raghu

We’ll be presenting CryoBoltz ❄️⚡️ at #NeurIPS this week! Check out the new experiments in our updated paper. We’re also releasing our code for the community to try out on their data 🚀 Paper: arxiv.org/abs/2506.04490 Code: github.com/ml-struct-bio/…

Rishwanth Raghu kicks off our BioE Lunch and Learn 🍕 series for the semester! Rish is a graduate student in @ZhongingAlong’s lab and presenting “Multiscale guidance of AlphaFold3 with heterogeneous cryo-EM data”

@gabriel_a_d_ Thanks Gabe!

@rishwanth_raghu @axlevy0 @GordonWetzstein @ZhongingAlong EPIC

Excited to present CryoBoltz ❄️⚡, a multiscale guidance approach for steering AlphaFold3/Boltz-1 to sample structures that are consistent with experimental cryo-EM density maps. 🧵1/7 arxiv.org/abs/2506.04490 Joint work with @axlevy0 @GordonWetzstein & @ZhongingAlong!

@venzelele Thanks! We did not try other data modalities, but SAXS or any other measurement with a differentiable forward model could be used to define a new guidance target

@rishwanth_raghu @AllThingsApx @axlevy0 @GordonWetzstein @ZhongingAlong Great work! Interesting, can it be used for SAXS data?

@algobaker Thank you!

@rishwanth_raghu @axlevy0 @GordonWetzstein @ZhongingAlong Awesome paper and very well written thread

@MazAbulnaga Thank you Maz!

@rishwanth_raghu @axlevy0 @GordonWetzstein @ZhongingAlong Congrats, Rish! This is amazing work

For a detailed description of our approach and analysis, check out our preprint! Predicting conformational dynamics is a key challenge for structural biology. CryoBoltz is our attempt to unlock the full potential of structure prediction models using experimental data. 🚀 7/7

We also demonstrate our method on real cryo-EM data of P-glycoprotein. CryoBoltz models 4 conformational states of this transporter given experimental maps, whose resolutions are too low (> 4Å) for model building algorithms. 6/7

🚨New paper at #NeurIPS2024! We present Hydra, a mixture of neural fields 🐉🐉🐉 for ab initio cryo-EM reconstruction of complex mixtures. Hydra is our attempt at pushing the boundaries for even more extreme forms of heterogeneity in cryo-EM 🤯. This was joint work with an awesome team, led by @axlevy0 @rishwanth_raghu & David Shustin. Paper: hydra.cs.princeton.edu Poster session happening now! Come by 👋