Jared Schrader

Our lab is excited to share our latest preprint describing the BR-body proteome with huge help from @wschilders44 @JulieBiteenLab @ChampionNd and Elaine Zhu's lab @WayneStateEng @WSUBioSci biorxiv.org/content/10.110…

Stress Changes the Bacterial Biomolecular Condensate Material State and Shifts Function from mRNA Decay to Storage biorxiv.org/cgi/content/sh… #biorxiv_biophys

Polyphosphate affects cytoplasmic and chromosomal dynamics in nitrogen-starved Pseudomonas aeruginosa | PNAS pnas.org/doi/10.1073/pn…

A rapid inducible RNA decay system reveals fast mRNA decay in P-bodies: nature.com/articles/s4146…

A conserved morphogenetic module that controls complex bacterial cell shapes. elifesciences.org/articles/86577…

Genome-wide profiling of Hfq-bound RNAs reveals the iron-responsive small RNA RusT in Caulobacter crescentus | mBio journals.asm.org/doi/10.1128/mb…

I’m excited to share my first postdoctoral work where we captured the first high resolution structure of the bacterial magnesium transporter MgtA! This project allowed me to learn cryo with @DoreenMatthies, molecular simulations with @AlexSodt and function with @storzlab.

Congrats to former postdoc Felix Keber from our & @MartinWuhr lab on this fascinating paper using filtration experiments w mass spec to show that ubiquitous mesoscale condensate formation across >18% of the proteome. rdcu.be/dzXsA

Our story on tracking the in vivo NusG whereabouts is on @MolecularCell! NusG rarely walks alone, enjoys 30S company, works out on anti-terminating and TT-coupling. True saga by brilliant @HAFEZELSAYYED, super support by @ojp25 @M_Stracy and Max Gottesman! sciencedirect.com/science/articl…

Thrilled to finally announce our work on the selectivity of disordered protein condensation in @NatureChemistry. Great collaboration with @JeetainMittal Led by @rmwelles and @kandarp_sojitra. rdcu.be/dxP4r. See related work by Brangwynne lab rdcu.be/dzddw

Excited to see beautiful manuscript led by @RanaUshnish & Kevin w @iAmAllAdhesion @M_T_Walls @javalosLAB & AZP out in @NatureChemistry (...after 5 mo. copyediting :( rdcu.be/dzddw Also see related co-submission by @buenoscience & @JeetainMittal rdcu.be/dxP4r

Interested in the mechanisms driving subcellular organization in bacteria? The Vecchiarelli lab has a postdoc position available! docs.google.com/document/d/1jH… Plz RT!

Happy to see this one in the print 🙂 We describe how disordered prion-like domains can drive selective co-condensation of fusion oncoproteins with chromatin remodelers😎 Postdoc @richoodavis led this work with two talented grad students. 👏👏👏 nature.com/articles/s4146…

How does cellular stress affect #RNA? We sequence entire RNA molecules with @nanopore sequencing. We find 👉Global RNA decay mediated by XRN1, independent of poly(A) tail! 👉Decay absolutely depends on stress-granule formation! biorxiv.org/content/10.110…

Great to participate in this led by @HymanLab, Simon Alberti, @JeetainMittal and w/ @AyalaLab1, @titus_franzmann probing how cysteine oxidation aggregates TDP-43 Intra-condensate demixing of TDP-43 inside stress granules generates pathological aggregates biorxiv.org/content/10.110…

This is a very exciting paper with one of the clearest applications so far to take advantage of bacterial single-cell RNA-seq's ability to pull apart heterogenous gene expression within populations. Highly recommend! nature.com/articles/s4158…

We have a new story out at BiorXiv! Amazing work led by @za_curtis (Bisson), Pedro Escudeiro (Alva) and John Mallon (Bisson). Great collaboration with @Vikram_Alva (Max Planck) and @pohlschr (UPenn). A summary of our findings in the thread (1/22) shorturl.at/fLPR1

What determines polar mRNA localization in E. coli? Shanmugapriya Kannaiah, @OChoder et al @Hujimed find the polar transcriptome to depend on MinD inhibition of RnaseE recruitment embopress.org/doi/full/10.10…

In a new paper in @Nature, an impressive and creative series of insights led @DDunkelmann and coworkers in Jason Chin’s lab to develop a powerful, general in vitro selection for aminoacyl-tRNA synthetase enzymes that charge tRNAs with a wide variety of small molecules, including those not necessarily compatible with known ribosome variants (yet). They used this system to create aaRSs that enable beta-amino acids and alpha,alpha-disubstituted amino acids to be site-specifically incorporated into proteins, solved the structure of GFP containing meta-brominated beta-phenylalanine as the third residue as confirmation, and advanced the non-canonical biopolymer field forward in the process. Wow. Brilliantly explained in the thread below. 👏👏🍻

@AmyPalmerZinc @SmithLabUMBC There's two commercial solutions people suggested that have up to 3mm spacers, but one of my old Uhlenbeck lab-mates built custom rigs like Olke's us.vwr.com/store/product/… cbsscientific.com/collections/ve…

Anyone able to recommend vertical PAGE rigs for preparative RNA purification after T7 transcription? I inherited all my PhD Adviser Olke Uhlenbeck's old gel rigs when he retired, but they were all custom built, so I need to find commercially available replacements.

Late consolidation of rRNA structure during co-transcriptional assembly in E. coli by time-resolved DMS footprinting biorxiv.org/cgi/content/sh… #bioRxiv