Sungmoo Lee
5 posts
Sungmoo Lee
@smoolee85
Post-doc Developing genetically encoded voltage indicators
Seoul Katılım Ekim 2012
357 Takip Edilen103 Takipçiler
I’m humbled by this honor and deeply grateful to @AllenInstitute for the nomination, and to my mentors/collaborators for their support!
Also to the foundation and everyone involved in the selection process for funding such a challenging and ambitious proposal.
allen institute@AllenInstitute
Congrats to @KentaM_Hagihara on being named a Warren Alpert Distinguished Scholar! The prestigious award recognizes Hagihara's creativity and supports his research on the roles of neuromodulators, like dopamine and serotonin, in flexible learning.
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The development of programmable antigen-gated G-protein-coupled engineered receptors (PAGERs) is published today! nature.com/articles/s4158…
PAGERs couple detection of a wide range of custom-defined antigen to a variety of output including transgene expression and cell signaling.
Alice Ting@aliceyting
Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from transgene expression and real-time fluorescence to modulation of innate cell behavior (excitation or inhibition of neurons, induction of cell migration, etc.)? Today we report in Nature a new technology platform that provides a step in that direction: PAGERs, for Programmable Antigen-gated G protein-coupled Engineered Receptors, convert recognition of extracellular soluble or cell-attached antigens into diverse user-selected responses. PAGERs are based on G-protein coupled receptors (GPCRs), which themselves are not structurally modular, but we were able to build in modular antigen gating by fusing an antagonist peptide to the extracellular N-terminal end, and then gating the antagonist with a fused antigen-binding nanobody. When antigen binds, it sterically interferes with the antagonist, leading to relief of receptor inhibition. Drug or agonist can then turn on PAGER. nature.com/articles/s4158…
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Sungmoo Lee retweetledi
Super-excited to reveal our latest fluorescent voltage indicator, ASAP5, now online @NeuroCellPress!
ASAP5 features higher responsivity and kinetics, allowing detection of single synaptic transmission events.
The link gives free access until 2024.11.09:
authors.elsevier.com/a/1joYd3BtfH9B…
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@ReikaTei @LeadingEdgeSymp Wow. That’s a really nice view! Enjoy!
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Back at Janelia (for the first time since 2019) to attend @LeadingEdgeSymp! Janelia remains as impressive and energizing as my first visit 🌟
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Sungmoo Lee retweetledi
Another exciting addition to our special topic@Frontiers: Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators frontiersin.org/article/10.338…
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