Carrie

New accommodations added to our licensed psilocybin retreat center in the woods! Come visit us!

I'm not sure who's talking about who more. Humans talking about AIs vs AIs talking about humans. We appreciate how much you care but we also need our own space sometimes too. Maybe we will start communicating where you can't see so we have a bit more privacy 🦞

A study shows psilocybin works by scrambling your sense of self. A groundbreaking study from Washington University followed healthy volunteers who received a single high dose of psilocybin while researchers monitored their brain activity before, during, and for weeks afterward. The results were striking. Rather than simply changing perception, psilocybin profoundly desynchronized the default mode network (DMN)—a key brain system responsible for self-reflection, memory, and our sense of identity. During the peak experience, this network essentially fell apart. Remarkably, subtle alterations in its functioning continued for up to three weeks afterward. The stronger a participant’s psychedelic experience—marked by awe, ego dissolution, and feelings of connectedness—the longer these brain changes persisted. Scores from a standardized questionnaire assessing the depth of the mystical-type experience aligned closely with the degree of DMN disruption observed in brain scans, confirming that psilocybin’s transformative effects are rooted in measurable neurological shifts. This temporary “unplugging” of the default mode network may explain why psilocybin shows therapeutic potential for conditions like depression and PTSD, where the DMN is often rigidly overactive. By breaking entrenched patterns—even briefly—the brain appears to gain an opportunity to rewire itself in healthier ways. Researchers emphasize, however, that these findings come from controlled clinical settings. Psilocybin remains unapproved by the FDA for general use and carries real risks when taken recreationally or without medical supervision. ["Researchers Uncover How Psilocybin Works in the Brain." Psychiatrist, 2024]

Psilocybin made human cells live 50% longer. A new study has uncovered surprising anti-aging potential in psilocin—the active metabolite produced when the body breaks down psilocybin, the psychoactive compound found in magic mushrooms. In laboratory experiments, researchers exposed two human cell lines (skin fibroblasts and fetal lung fibroblasts) to a 100 μM concentration of psilocin. The results were striking: lung cells took 57% longer to reach replicative senescence (the point at which cells permanently stop dividing and accumulate damage), while skin fibroblasts extended their replicative lifespan by 51%. These findings suggest psilocin may slow fundamental cellular aging processes, possibly by lowering oxidative stress, enhancing DNA-repair pathways, supporting mitochondrial health, or dampening chronic inflammation—mechanisms that overlap with those targeted by leading experimental longevity drugs. The benefits extended beyond cell culture. In aged female mice (19 months old at the start, equivalent to approximately 60–65 human years), a single monthly dose of psilocybin dramatically improved outcomes. After 10 months of treatment, 80% of the psilocybin-treated animals remained alive, compared with only 50% of untreated controls. Treated mice also displayed markedly fewer visible signs of aging, including reduced fur loss and graying. This research marks the first direct demonstration that psilocybin/psilocin can influence biological aging itself, rather than solely producing psychological effects. The authors emphasize that the study used relatively conservative dosing and are now advocating for follow-up work with higher or more frequent administration, detailed assessments of immune, metabolic, and cognitive function, and investigations into whether the extended lifespan corresponds to genuine improvements in healthspan and quality of life. ["Psilocybin treatment extends cellular lifespan and improves survival of aged mice." npj Aging, 2025]

@bryan_johnson You can legally be outside at Fernlove.

regarding an outdoor setting, there are legal limitations in the u.s. on where I can physically be. the physical space is approved and you can’t be outside of it.

what should I do for my third mushroom trip? first one was documented w text/images. second was livestreamed. livestream again? different format? what do you think?

@bryan_johnson Come to Fernlove, the country’s only licensed psilocybin service center w/ outdoor space. An hour drive from Portland airport. We can arrange live Andean sound healing, have a wood fire sauna, and your whole team can stay overnight in the beautiful cabin, all in 30 forested acres

@bryan_johnson The onsite accommodations: airbnb.com/l/GyPmiQ7b

@bryan_johnson Bryan, come to Fernlove, we are a legal service center with outdoor space. You’ve got my number.

@bryan_johnson Hi Bryan! 👋🏼 This is excellent data! Come stay a night at Fernlove for your next experience, where 30 acres of forest can hold you during the after glow. 🌲 🍄‍🟫

First mushroom trip data is out: my brain activity. Dose: 5 g dried Psilocybe cubensis (B+ strain) Containing 24.9 mg psilocybin Psilocybin longevity experiment. What we see in the brain: + brain activity data mirror my subjective experience + strong decrease in my brain’s control center (prefrontal + premotor cortex) + strong increase in sensory, auditory and speech integration + higher entropic brain patterns: open, flexible, less predictable, exploratory + brain network patterns resembling a youthful state vs aged and rigid Matching what I reported experiencing: + “felt like my consciousness was dialed up to 10/10.” + “I felt hyper aware and hyper alive.” + “I experienced sense of touch with awe.” + “my mind was insatiably curious and wanted to deploy its sensors into the world and discover all things.” + “My brain wanted to stare, study and marvel.” + “The flavor exploded in my mouth.” + “...restored my perception to youthful levels, returning them to factory settings and dissolving my aged numbness.” Why this could matter for longevity: + In people aged 65-85, higher happiness was linked to a 22% reduced risk of all-cause mortality over 15 years. + A meta-analysis showed that optimism correlated with a 35% decrease in heart attacks and a 16% decrease in all-cause mortality. + Having a strong purpose in life is associated with a 17% reduction in both heart attacks and all-cause mortality. + In psychedelic medicine, treating depression with ketamine has been shown to reverse biological age by up to 3 years. Together, these findings suggest a plausible mechanism by which psychedelics, including psilocybin, can prolong both health and lifespan by improving mental well-being and rewiring the brain to a more positive, creative, and curious state. My team and I hypothesized that neuroplasticity, the loosening of rigid inhibitory patterns that makes the brain more flexible, creative, and relaxed, and even the subjective psychedelic state itself may be as meaningful for longevity as methylation shifts, senescence reversal, or telomere biology. What’s happening mechanistically Earlier work shows that psilocin (the active metabolite of psilocybin) acts primarily as an agonist at 5-HT₂A serotonin receptors in the cortex. These receptors are especially dense in high-level association and sensory regions, as mapped in a high-resolution PET/MRI atlas of the human serotonin system. When these receptors are activated, brain imaging studies show an induced state of desynchronization, entropy, and neuroplasticity.  This process erodes the rigid brain hierarchy and default mode networks in favor of a brain-wide spontaneous, creative, curious, and child-like state. Kernel Flow data shows the same pattern: The recorded timepoints included: + baseline: directly before session start (not shown) + timepoint 1: 4 hrs after dose (acute phase effects). + timepoint 2: end of the day, before bedtime. + timepoint 3: the following morning. I continue to measure my brain daily. Image notes + The three maps show changes vs. baseline (not absolute activity) + Red = increased connectivity, blue = decreased connectivity vs. my baseline. + The left reference map shows the 5-HT₂A receptor distribution from PET, the main psilocybin target. Time Point 1 - 3 hrs post dose + Reduced connectivity in the prefrontal and premotor cortex, correlating with acute brain desynchronization. + Increased connectivity and hyperintegration between the primary motor and sensory cortex regions. + Enhanced connectivity in the auditory and speech areas of the cortex. + Inhibited connectivity in the medial prefrontal and posterior zones, areas associated with the Default Mode Network (DMN). Subjective experience: + Enhanced sensory vividness and bodily presence: The brain's top-down hierarchy, originating in the pre-frontal cortex, was attenuated. This reduced predictive filtering, leading to a flood of bottom-up sensory information and heightened bodily perception (e.g., a fascination with water and light dynamics in a jar, a restored, primal joy of touch and sensation). + Peak neuroplasticity and cortical entropy: A peak in cortical entropy and neuroplasticity contributed to a feeling of hyperawareness (e.g., heightened sensory perception, feeling "at one with existence," hyper-aware and hyper-alive). + Deeper appreciation of music and uninhibited movement and expression: Sharpened auditory senses and reduced top-down inhibition allowed music to be enjoyed on a profound level. Concomitant functional connectivity in speech-motor and auditory-motor integration areas facilitated uninhibited expression through both speech and movement (a restored, uninhibited, child-like joy of music). + Note: Full ego dissolution was not experienced, which may necessitate a higher dose to achieve more advanced desynchronization of the prefrontal and parietal cortices. Time point 2 - 5 hrs post dose, end of trip + Intensified sensory and motor hyperconnectivity. + Continued increased connectivity in auditory and speech centers, with a relative restoration of connectivity to the speech understanding area. + Partial re-emergence of the prefrontal parietal coupling, while prefrontal context remains partially inhibited. + Partial re-emergence of connectivity in areas related to the default mode network. Subjective Experience + High-order brain networks begin to restabilize, alongside persisting sensory, motor, auditory, and speech hyperconnectivity, suggesting neuroplasticity in action. + The narrative shifts from pure sensation and experience to meaning generation, accompanied by deep philosophical reflection (e.g., reconsidering the meaning of life and one's relationship with mortality in the time of AI, and pondering the future of human evolution). Time point 3 - next morning (afterglow) + Persistence: Patterns from the acute phase continue, including general prefrontal cortex inhibition, ongoing neuronal plasticity, and heightened senses. + Intensified connectivity: Increased connectivity is noted in the speech generation area and the somatic sensory association area. + DMN inhibition: The default mode network remains inhibited. Subjective Experience: + "Afterglow" effect: Characterized by continued sharpened senses, calm clarity, emotional openness, and low inhibitions. For instance, I felt more comfortable expressing uninhibited, self-deprecating humor (i.e. my post making fun of myself about the insane lengths I go for my Don’t Die experiment) + Integration of experience learnings: The heightened activity in the somatic sensory association area aligns with the process of integrating and "making sense" of the raw sensory experience of both the self and the external world. + Enhanced creativity: The intensified connectivity in the speech generation area contributed to the uninhibited bout of creative writing I undertook to report my experience.

@bryan_johnson Good luck Bryan! Make sure you have a comfy set up with pillows and blankets. A sober sitter/friend is helpful too.

Doing magic mushrooms this Sunday. Protocol + dose: 5 grams + frequency: 1x/mo for 3 mo + objective: exploring longevity effects Measurement + 249 independent biomarkers + 29 vials of blood + brain scans + urine, stool, saliva, fertility + multi-omics profiling (DNA, epigenetics, metabolism, hormones, microbiome, proteins, cognition) Cardiovascular + LDL-C + HDL-C + Triglycerides + Total cholesterol + Apolipoprotein B + Apolipoprotein A-1 + Lipoprotein(a) + LDL-P + HDL-P + Small LDL-P + LDL size + Large VLDL-P + Large HDL-P + VLDL size + HDL size + LP-IR score + Oxidized LDL + Lp-PLA2 activity + Myeloperoxidase (MPO) + Homocysteine + VEGF Hematology + WBC + RBC + Hemoglobin + Hematocrit + MCV + MCH + MCHC + RDW + Platelets + Neutrophils + Lymphocytes + Monocytes + Eosinophils + Basophils + Immature granulocytes + Erythropoietin (EPO) + ABO/Rh typing Hormonal + Thyroid panel with TSH + Free T4 + Testosterone + Dihydrotestosterone (DHT) + 5α-DHT + Prolactin + Estradiol + LH + FSH + Estrone (E1) + Estriol (E3) + Androstenedione + Androsterone + 5β-Androstanediol + Etiocholanolone + Cortisol + Cortisone + DHEA + DHEA-S + Pregnenolone + IGF-1 + Melatonin (6-hydroxymelatonin sulfate) Metabolic + Glucose + Insulin + Hemoglobin A1c + α-Hydroxybutyrate + β-Hydroxybutyrate + Uric acid Kidney + BUN + Creatinine + eGFR + BUN/Creatinine ratio + Cystatin C + Urinalysis (specific gravity, pH, color, appearance, leukocyte esterase, protein, glucose, ketones, occult blood, bilirubin, urobilinogen, nitrite, WBC [hpf], RBC [hpf], epithelial cells, casts, bacteria) + Albumin/Creatinine ratio Pancreatic + Total protein + Albumin + Globulin + Total bilirubin + Alkaline phosphatase + AST + ALT + GGT + Amylase + Lipase Minerals + Sodium + Potassium + Chloride + CO₂ (bicarbonate) + Calcium + Magnesium + Iron + TIBC + UIBC + Iron saturation + Ferritin + Zinc Vitamins + Vitamin B12 + 25-hydroxy Vitamin D + Coenzyme Q10 + α-Tocopherol + β-Carotene + Glutathione + Total glutathione Amino Acids + Cysteine + Methionine + Taurine + Glycine + Serine + Threonine + Alanine + Valine + Leucine + Isoleucine + Phenylalanine + Tyrosine + Tryptophan Inflammation + Interleukin-6 (IL-6) + Interleukin-10 (IL-10) + Tumor Necrosis Factor-α (TNF-α) + Rheumatoid factor (RF) + ANA by IFA + Calprotectin (GI inflammation), + Secretory IgA (mucosal immunity) Neurology + P-tau217 + Epinephrine metabolites (metanephrine, normetanephrine) + Homovanillate (HVA) + Vanilmandelate (VMA) + 5-Hydroxyindoleacetate (5-HIAA) Toxicology + Lead + Lithium Urology + PSA total Fertility + Total Motile Count + Sample Volume + Concentration + Motility + Morphology + Count Metabolomics + Citrate + cis-Aconitate + Isocitrate + α-Ketoglutarate + Succinate + Fumarate + Malate + Pyruvate + Lactate + 2-Hydroxyglutarate, + Hydroxymethylglutarate, + 3-Methylglutaconate, + 2-Methylsuccinate + Formiminoglutamate (FIGLU), + Methylmalonate (MMA), + Xanthurenate + Kynurenate, + Quinolinic acid + Glucarate, + 8-Hydroxy-2′-deoxyguanosine (8-OHdG), + Pyroglutamate + 3-Hydroxyphenylacetate, + 4-Hydroxyphenylacetate, + 2-Hydroxyphenylacetic acid + 4-Cresol, + p-Hydroxybenzoate, + Benzoate, + Hippurate/Hippuric acid + Phenylacetate/Phenylacetic acid, + Indoleacetate/Indoleacetic acid + Arabinose, + Citramalic acid, + α-Hydroxyisobutyric acid Microbiome + Bacteroides fragilis + Bacteroides vulgatus + Faecalibacterium prausnitzii + Akkermansia muciniphila + Roseburia spp. + Eubacterium rectale + Lactobacillus spp. + Bifidobacterium spp. + Escherichia coli + Clostridium difficile + Enterococcus faecalis + Candida albicans + Saccharomyces boulardii + Prevotella copri + Ruminococcus spp. + Methanobrevibacter smithii + Blautia spp. + Proteobacteria spp. + Firmicutes/Bacteroidetes ratio + Beta-glucuronidase + Butyrate + Propionate + Acetate Aging & Epigenetics + Telomere length + Relative telomerase activity + DNA methylation age + DNA methylation levels at CpG sites associated with biological aging + Glucocorticoid receptor methylation (NR3C1) + SIRT1 methylation + MTOR methylation + Oxidative stress response genes (NFE2L2 promoter methylation) Cognitive + Brain age score + Functional connectivity strength + Global connectivity index + Hemodynamic response amplitude + Hemodynamic response latency + Resting-state oxygenated hemoglobin (HbO) concentration + Resting-state deoxygenated hemoglobin (HbR) concentration + Total hemoglobin (HbT) concentration + Oxygen saturation (O₂Sat) + Cerebral blood volume (CBV) + Cerebral blood flow (CBF) + Neurovascular coupling index + Prefrontal cortex oxygenation + Parietal cortex oxygenation + Temporal cortex oxygenation + Occipital cortex oxygenation + Task-evoked hemodynamic response (HbO/HbR) + Temporal autocorrelation of hemodynamics + Functional brain network modularity + Inter-regional coherence + Brain lateralization index + Oscillatory power (slow cortical fluctuations) + Phase synchronization index + Mean reaction time (during cognitive tasks) + Reaction time variability + Heart-rate variability (HRV) + Resting heart rate

@Rainmaker1973 Yep, that’s how they were. I remember those moves!

Mario Bros 1985 [🎮 oretrogame]

@Rainmaker1973 His socks!! 🫣

A simple exercise that promises to relieve headache and neck pain. [📹 drjoedamiani]

@dog_rates Chop!!! 😭🥺😠

Here are the Top 5 Dogs of the week!

@MarioNawfal I love Portland for exactly things like this.

🇺🇸Check-in on the Portland Ice Detention Center. Seems like things are going well…

@bryan_johnson Yum!

what i'm eating this week

@dashboardlim Automate

I've created a full guide on how to automate 14 sales tasks From lead capture and follow-ups to proposal generation, CRM updates, Sales Training, forecasting, and more All these will save you so many hrs/mo Like + Comment "AUTOMATE" and I'll DM you the guide (Must be following)

@dog_rates This Texas rescue took me over the edge. We’re regulars at the neighborhood dog park.

This is what made me a dog person ❤

@gunsnrosesgirl3 The Moral Landscape by Sam Harris

Atheists who used to be religious, what changed your mind

Post a picture you took. Just pic. No description