Sudhamsu

Disulfide constrained Fabs overcome target size limitation for high-resolution single-particle cryo-EM biorxiv.org/cgi/content/sh… #biorxiv_biophys

This could potentially enable rapid structure determination of antigen-Fab complexes for antibody based therapeutics and also enable small molecule drug discovery when a crystallography based structure system is not available.

If one can obtain a Fab that binds to a target of interest, applying our #RigidFab mutations will substantially increase chances of: (a)   Obtaining a high-resolution structure of the small target (b)   Improving resolution for existing Fab-target complex structures

Thrilled to share a pre-print describing an elegant solution to overcome target size limitation of single particle #cryoEM, where we demonstrate high resolution (2.3 – 2.5 Å) structure determination of small (~20 kDa) ordered proteins using cryoEM! biorxiv.org/content/10.110…

We have an exciting opportunity for a postdoctoral fellowship in Structural Biology and biochemical / biophysical characterization of protein complexes in the Wnt signaling cascade. Come join our teams and perform impactful research! tinyurl.com/GNESBPD

We have an exciting opportunity for a Postdoctoral Fellow in Structural Biology, to better understand molecular mechanisms of signaling in the WNT pathway: careers.gene.com/us/en/job/GENE… Please reach out directly if you are interested!

@simanshu Congratulations to your entire team @FredNatLab on this publication, @simanshu. Nice and detailed work that complements recent similar work from others in the field!

Finaaaally after months of editorial and production delays, our manuscript describing the structure of the SHOC2-MRAS-PP1C complex is published online at Nature Structural and Molecular Biology! nature.com/articles/s4159…

Our work on the SHOC2-MRAS-PP1C complex adds to the beautiful work done on this complex by @sudhamsuj at Genentech, @JKwonBio @andrewaguirremd Bill Hahn at Dana Farber, Hauseman, et al. @Novartis!

Our work and recent work by @simanshu group @NCI; @sudhamsuj group @genentech; Hauseman et al @Novartis help shed light on the structural basis of SHOC2 engagement with PP1C and RAS (MRAS & K/H/NRAS). (3/n) nature.com/articles/s4158… twitter.com/sudhamsuj/stat… biorxiv.org/content/10.110…

Happy to share our latest work now out in @nature. At long last, we resolved the structure of the SHOC2-RAS-PP1C complex and integrated this work with deep mutational scanning to comprehensively evaluate #SHOC2 structure and function. 🧵(1/n) nature.com/articles/s4158…

Nature research paper: Structural basis for SHOC2 modulation of RAS signalling go.nature.com/3QWNjVr

In @Nature, our scientists describe how a protein, SHOC2, activates RAF by bringing together two key components RAS and PP1C. Many RAS dependent tumors also depend on SHOC2, highlighting a potential way to target the RAS-RAF pathway by targeting SHOC2. go.nature.com/3uepuyJ

@genentech @Nature @gerosa_luca @NickLiau @AACR

Our structural and biochemical insights pave the path for potential ways to target SHOC2 to intervene in certain RAS mutant tumors!

I am thrilled to share our recent publication in @Nature, on the Structure of the SHOC2:PP1C:RAS complex, unraveling a lingering 20 year old mystery in the most mutated oncogenic pathway in all cancers. nature.com/articles/s4158…

An excellent opportunity to join the Structural Biology department at Genentech as a Senior Scientist, to enable drug discovery and research. careers.gene.com/us/en/job/GENE…