Tarek

@DjuranovicLab @slavicaPD This is a game changer for the field Sergej, highly recommended!

Our RAPPL international patent application has just been published. @slavicaPD

@FlorentWaltz @jomaa_lab @ScienceMagazine @luminous_lab Congrats to that story. And the cover page as well 🍾🎉

The final version of our work on the in-cell architecture of the mitochondrial respiratory chain is now online in @ScienceMagazine 🎉 You can find the full story here science.org/doi/10.1126/sc… @luminous_lab did a wonderful job on the cover and animation👩‍🎨

And again, intriguing structures of the large ribosomal subunit @RainerNikolay

@DjuranovicLab @slavicaPD @Hashemyaser4 Game changer

Collaborative work of @slavicaPD, @Hashemyaser4, Jovanovic Lab and @DjuranovicLab - a new method to isolate ribosomes and translational material in minutes. We show multiple usage of our RAPPL method in biochemical and structural assays with ribosomes. Please be free to contact us for further instructions if needed. @IsraelF96135088 you'll like this one. A rapid, facile, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies biorxiv.org/content/10.110…

@HiCryoEM @Mark_A_Nakasone Keep us informed on the performance. Too few Apollos out there in the jungle yet.

@Mark_A_Nakasone Bingo! Detector will be DE Apollo. I have had enough trouble with Gatan.

Guess what’s in the box?

@IsraelF96135088 😅

@tarekh247 no, Krios after this summer surgery

ApoFe testing going OKish…1.7A not yet finish… it was not the thinnest grid but seems the scope is OKish after severe surgery. Also in house gold grids seem to work.

@LabConti @MPI_Biochem Congratulations, finally a structure of ribosome associated Ski7.

Excited to share our latest research on co-translational mRNA decay by the exosome in human cells! We uncover how the exosome-SKI complex is recruited to ribosomes and propose a novel role for SKI in recognizing collided disomes. @MPI_Biochem 👇👇👇

I never realized acknowledgements for reviewing activities in papers. Is this new? Who actually reveals themselves? Asking for a friend...

@MishaKudryashev @v_mikirtumov How about good old shadowing if you're not working in situ?

The handedness of cryo-EM structures: unless the structure is high resolution - there is a 50% chance of getting the handedness wrong. Me and @v_mikirtumov recently co-authored a paper where it was needed (a thread) cell.com/structure/full…

@BJ_Greber @OliBClarke @IsraelF96135088 @gonenlab @hervecelia @Guillawme 🍿 reliable, unbiased and resolution in one sentence 👀

What software is most popular these days in single particle cryoEM? I saw at least one paper from a group that developed one program now using someone else program. Comments welcome regarding this or that.

@IsraelF96135088 Good stuff

Dear X-friends, Now that I am a poor scientist in a country where science is not a priority, I have to be careful with my expenses. I saw that NanoSoft sells (cheaper) auto grids and C-clips, anyone has experience with them? As good as TFS or crazy idea to buy them? Thanks!

@IsraelF96135088 @v_paavilainen Why not go for graphene/o ?

@v_paavilainen A bit too thick carbon, the contrast could be improved I think but yeah… could be worse!

First 60S on homemade carbon… not perfect but getting there…

@ChristosGSavva Impressive

Benchmarking the Krios G4 here at KAUST gave us this nice 1.42Å map. Waters, hydrogens and waters with hydrogens. See more here: biorxiv.org/content/10.110…

@daniel_roderer @LeibnizFMP @FelixSchoepf @CFcryo Finally, congrats Daniel and team!

I’m proud to present the first preprint of my lab @LeibnizFMP, reporting how Fusobacterium nucleatum binds to the human host via its adhesin Fap2: biorxiv.org/content/10.110… A big thanks to @FelixSchoepf, @CFcryo , Gian Luca Marongiu, Klaudia Milaj, Judith Kikhney and Annette Moter.

@hauke_hillen @paulafprado Glückwunsch Hauke, großartig.

Happy to share the results of a great collaboration with Thomas Pfannschmidt (LU Hannover), led by the amazing @paulafprado and Frederik Ahrens. Together, we determined the structure of the multi-subunit plastid-encoded RNA polymerase from chloroplasts! cell.com/molecular-cell… 🧵

This is huge. Congrats Hauke and team.

@BJ_Greber Same particles gave better reconstruction with blush? Changes in 3DFSC, SCF etc. for the reconstructions?

@BJ_Greber Did you apply polishing in relion vs cryosparc? Would be interesting to compare refinement results of identical particle sets.