UNC-NeuroTools

@VincentCostaPhd Thanks @VincentCostaPhd! Our work isn’t done till these viruses work for you. Glad they are, but if you ever have an issue, know our job isn’t done till it works. Thanks to the BRAIN Initiative for their critical U24 support.

If you aren’t getting your viruses produced by @uncneurotools you’re wasting your money. Strongly endorse their services.

@uncneurotools is looking for a tech! Join us to learn about viral vectors in gene therapy and neuroscience! unc.peopleadmin.com/postings/245760

@FreglisPlethon unc-neurotools.org

@uncneurotools Thank you. Could you post the link?

We can take on a few more labs to fine tune our logistics. Turn around time for AAVs is 3-4 weeks from when we receive your DNA to virus back in your hands. At just under $500/100ul for neuro applications, it’s a pretty sweet deal (thank the BRAIN Initiative).DM for more details!

@FreglisPlethon Yes, whatever payload you send us in the capsid of your choice! Please@just make sure the DNA is has intact ITRs, EF-free, and if purified by isopropynol ppt, you have done a EtOH ppt clean-up.

@uncneurotools Just to be clear under 500 for stocks not for new ones. For example if I send you DNA is under 500?

@SA_Diabetes @KimRitola @superkash We haven’t really seen a difference based on capsid. Expressed payload during virus production effects are greater.

@KimRitola @superkash @uncneurotools Any AAV type better than others for larger plasmids?

Anyone had major problems with AAV titer when exceeding the packaging limit? I know some AAVs can take larger plasmids than advertised. I want to add an IRES-EGFP to a plasmid but it will be >>4.7 kb. I'm not too keen on using a 2A link instead.

Come see us at #SfN22! Join us Sunday 11/13 6:30-9pm at the BRAIN Initiative Alliance Social for Tools, Tech and Theory. braininitiative.org/events/sfn-soc… #studyBRAIN

@k_pietersz @plasmidsaurus Works well for finding deletions outside the traditional restriction digest sites. Also very handy for looking at the homogeneity of your entire expression construct. Our prep will reflect the DNA quality. Bad in = bad out.

@uncneurotools @plasmidsaurus @plasmidsaurus For 15$ you also sequence ITRs? 🤯

Welcome to UNC NeuroTools! Formal request system to go live around June 9. Until then, get your EF DNA ready, QC constructs/ITRs (@plasmidsaurus) and register for an iLab account (for billing only-can do PO, check, or CC). Questions? Let's hear/share them....

Thanks neuro-community! Our Beta testers are set. Stay tuned for the next phase!

@Mayank_Verma_ Our cut-off is E12, but we typically get E13 across serotypes. If you need a specific titer we can adjust our protocol. We know some experiments require high titer/purity regardless of expression construct impact on titer.

@Andy__Osborne You just need to sign up for billing, order some viruses, and give us feedback on the process (mostly what's a pain or what's missing) including how well the viruses worked.

@uncneurotools How does the beta testing programme work?

We are looking for about 20 external to UNC volunteers to Beta test our billing and virus request systems. Please DM us if you are interested!

@vertigologist @MartinBohlen1 Yes, just be sure to know the customs requirements beforehand so nothing gets held up!

@uncneurotools @MartinBohlen1 Do you deliver overseas?

@VincentCostaPhd @btaylorblake the GOAT.

Histology is a talent not a skill.

@JustinGEnglish @nanopharmNC Yes, we specialize in custom!

@agetliang Hi Yajie! Happy to hear from you! Hope MD is treating you well. Send me your email and I will get you the info! Thanks!

@uncneurotools Hi I am interested (from university of Maryland):)

@GattoGraziana @KimRitola @uncneuro Customs can make it tricky. Do you have a customs agent you usually deal with? Not a bad idea to figure out exactly what they need before hand:)

@uncneurotools @KimRitola @uncneuro Fantastic! I had issues with other cores not able to ship rabies to Europe, and I know your are high quality 😃

UNC-NeuroTools is very close to being open to all. Follow @uncneurotools to see updates on registering an account and general info about viral vectors (AAV, rabies, and lenti) for neuroscience. Brought to you by @uncneuro and the BRAIN Initiative.

What makes our AAVs different? They are created with neuroscience applications in mind. Smaller volumes (100-500ul), custom capsid choices, and personalized vector production protocols to attain titer and purity necessary for results in YOUR experiment.

@superkash @KimRitola @uncneuro I am a big fan of rabies!